In Vitro Transposition System for Efficient Generation of Random Mutants of
            <i>Campylobacter jejuni</i>

Colegio, Oscar R.; Griffin, Thomas J.; Grindley, Nigel D. F.; Galán, Jorge E.

doi:10.1128/jb.183.7.2384-2388.2001

Cited by 37 publications

(32 citation statements)

References 18 publications

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“…not been well characterized, although several recent transposon mutagenesis experiments have demonstrated that Cj0041, Cj0062c, and Cj1026 are required for motility (17,29,50). For Cj1026 and Cj0062c, these motility defects may be caused by polar effects on the transcription of the downstream genes: flgR ( 54 activator) and fliA ( 28 ), respectively.…”

Section: Levels Of Genes Encoding Specific Flagellar Transcripts and mentioning

confidence: 99%

Genome-wide Expression Analyses of Campylobacter jejuni NCTC11168 Reveals Coordinate Regulation of Motility and Virulence by flhA

Carrillo

Taboada

Nash

et al. 2004

Journal of Biological Chemistry

189

174

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We examined two variants of the genome-sequenced strain, Campylobacter jejuni NCTC11168, which show marked differences in their virulence properties including colonization of poultry, invasion of Caco-2 cells, and motility. Transcript profiles obtained from whole genome DNA microarrays and proteome analyses demonstrated that these differences are reflected in late flagellar structural components and in virulence factors including those involved in flagellar glycosylation and cytolethal distending toxin production. We identified putative 28 and 54 promoters for many of the affected genes and found that greater differences in expression were observed for 28 -controlled genes. Inactivation of the gene encoding 28 , fliA, resulted in an unexpected increase in transcripts with 54 promoters, as well as decreased transcription of 28 -regulated genes. This was unlike the transcription profile observed for the attenuated C. jejuni variant, suggesting that the reduced virulence of this organism was not entirely due to impaired function of 28 . However, inactivation of flhA, an important component of the flagellar export apparatus, resulted in expression patterns similar to that of the attenuated variant. These findings indicate that the flagellar regulatory system plays an important role in campylobacter pathogenesis and that flhA is a key element involved in the coordinate regulation of late flagellar genes and of virulence factors in C. jejuni.

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Section: Levels Of Genes Encoding Specific Flagellar Transcripts and mentioning

confidence: 99%

Genome-wide Expression Analyses of Campylobacter jejuni NCTC11168 Reveals Coordinate Regulation of Motility and Virulence by flhA

Carrillo

Taboada

Nash

et al. 2004

Journal of Biological Chemistry

189

174

View full text Add to dashboard Cite

show abstract

“…1). Recently, successful and efficient transposon mutagenesis (90,91) has breached one of the early barriers to the explication of C. jejuni molecular pathogenesis. Together with the genomic sequence, which will provide the cornerstone for powerful novel techniques such as microarray and protein expression methodologies, these developments herald a renaissance period for Campylobacter research that can be expected to continue for some time.…”

Section: It Is Not Yet Clear What Role Cdt Plays In Infection In Vivomentioning

confidence: 99%

Enteric Campylobacter: Purging Its Secrets?

et al. 2004

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“…Also present in this organism is CheA, which contains both an HK domain and an RR domain. We did not generate a cheA mutant as the role of this gene in chemotaxis and motility (11,18,21) is well established. We were successful in generating mutants with mutations in 9 of the 11 RRs (Table 2).…”

Section: Generation Of Rr Mutantsmentioning

confidence: 99%

The Campylobacter jejuni Response Regulator, CbrR, Modulates Sodium Deoxycholate Resistance and Chicken Colonization

et al. 2005

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Two-component regulatory systems play a major role in the physiological response of bacteria to environmental stimuli. Such systems are composed of a sensor histidine kinase and a response regulator whose ultimate function is to affect the expression of target genes. Response regulator mutants of Campylobacter jejuni strain F38011 were screened for sensitivity to sodium deoxycholate. A mutation in Cj0643, which encodes a response regulator with no obvious cognate histidine kinase, resulted in an absence of growth on plates containing a subinhibitory concentration of sodium deoxcholate (1%, wt/vol). In broth cultures containing 0.05% (wt/vol) sodium deoxycholate, growth of the mutant was significantly inhibited compared to growth of the C. jejuni F38011 wild-type strain. Complementation of the C. jejuni cbrR mutant in trans restored growth in both broth and plate cultures supplemented with sodium deoxycholate. Based on the phenotype displayed by its mutation, we designated the gene corresponding to Cj0643 as cbrR (Campylobacter bile resistance regulator). While the MICs of a variety of bile salts and other detergents for the C. jejuni cbrR mutant were lower, no difference was noted in its sensitivity to antibiotics or osmolarity. Finally, chicken colonization studies demonstrated that the C. jejuni cbrR mutant had a reduced ability to colonize compared to the wild-type strain. These data support previous findings that bile resistance contributes to colonization of chickens and establish that the response regulator, CbrR, modulates resistance to bile salts in C. jejuni.

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In Vitro Transposition System for Efficient Generation of Random Mutants of Campylobacter jejuni

Cited by 37 publications

References 18 publications

Genome-wide Expression Analyses of Campylobacter jejuni NCTC11168 Reveals Coordinate Regulation of Motility and Virulence by flhA

Genome-wide Expression Analyses of Campylobacter jejuni NCTC11168 Reveals Coordinate Regulation of Motility and Virulence by flhA

Enteric Campylobacter: Purging Its Secrets?

The Campylobacter jejuni Response Regulator, CbrR, Modulates Sodium Deoxycholate Resistance and Chicken Colonization

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