In vitro transformation of cultured cells from Nicotiana tabacum by Agrobacterium tumefaciens

Márton, László; Wullems, G. J.; Molendijk, Lucy; Schilperoort, R. A.

doi:10.1038/277129a0

Cited by 248 publications

(58 citation statements)

References 13 publications

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“…The cocultivation method was first described by [28] and slightly modified according to [14]. KmR calli were selected in liquid or in solid medium as described previously [10].…”

Section: Bacterial Strains and Plasmidsmentioning

confidence: 99%

Efficient octopine Ti plasmid-derived vectors forAgrobacterium-mediated gene transfer to plants

et al. 1985

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A two-component cloning system to transfer foreign DNA into plants was derived from the octopine Ti plasmid pTiB6S3. pGV2260 is a non-oncogenic Ti plasmid from which the T-region is deleted and substituted by pBR322. pGV831 is a streptomycin-resistant pBR325 derivative that contains a kanamycin resistance marker gene for plant cells and a site for cloning foreign genes between the 25-bp border sequences of the octopine T-region. Conjugative transfer of pGV831 derivatives to Agrobacterium and cointegration by homologous recombination between the pBR322 sequences present on pGV831 and pGV2260, can be obtained in a single step. Strains carrying the resulting cointegrated plasmids transfer and integrate T-DNA into the genome of tobacco protoplasts, and transformed tobacco calli are readily selected as resistant to kanamycin. Intact plants containing the entire DNA region between the T-DNA borders have been regenerated from such clones. In view of these properties we present pGV831 and its derivatives as vectors for efficient integration of foreign genes into plants.

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“…The cocultivation method was first described by [28] and slightly modified according to [14]. KmR calli were selected in liquid or in solid medium as described previously [10].…”

Section: Bacterial Strains and Plasmidsmentioning

confidence: 99%

Efficient octopine Ti plasmid-derived vectors forAgrobacterium-mediated gene transfer to plants

et al. 1985

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“…Petit Havana SR 1 [10] were isolated from sterile shoot cultures grown on MS medium according to [11]. Transformation by protoplast cocultivation with A. tumefaciens GV3101 (pMP90RK) strains harbouring the different constructions, and regeneration of transformed plants were performed according to established methods [12][13][14].…”

Section: Plant Tissue Culture and Transformationmentioning

confidence: 99%

Construction of a heat‐inducible chimaeric gene to increase the cytokinin content in transgenic plant tissue

Schmülling¹,

et al. 1989

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The ipt gene of Agrobacterium tumefaciens T-DNA encodes an isopentenyltransferase which causes cytokinin overproduction and developmental alterations in transformed plants. A chimaeric gene constructed by positioning the ipt coding region under the control of the hsp70 gene from Drosophila melanogaster allows heat-regulated expression in transgenic plant tissue. Heat-shock treatment of tobacco calli transgenic for the chimaeric hsipt gene increases the endogenous cytokinin concentration and enables these calli to grow on cytokinin-free medium. Transgenic plants regenerated from calli transformed with the hsipt gene and grown at normal temperature are phenotypically normal.

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“…To ekplore these questions, we looked at the distribution of iron supplied to cultured plant cells inoculated with bacteria. Cells in suspension have often been used as model systems for investigation of plant responses to stress (Marton et al, 1979;Tong et al, 1986;Feistner, 1988). We also noticed that soybean cells were susceptible to the bacterial lytic attack, which is likely correlated with plant cell electrolyte loss (Brisset and Paulin, 1992) and may, therefore, be considered a representative system with which to pursue this analysis.…”

Section: Discussionmentioning

confidence: 99%

Iron Deficiency Induced by Chrysobactin in Saintpaulia Leaves Inoculated with Erwinia chrysanthemi

1993

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In this communication, we examine the fate of iron during soft rot pathogenesis caused by Erwinia chrysanthemi on its host, Saintpaulia ionantha. l h e spread of soft rot caused by this enterobacterium was previously shown to depend on a functional genetic locus encoding a high-affinity iron assimilation system involving the catechol-type siderophore chrysobactin. Leaf intercellular fluid from healthy plants was analyzed with regard to the iron content and its availability for bacterial growth. It was compared to the fluid from diseased plants for the presence of strong iron ligands, using a new approach based on the iron-binding property of an ion-exchange resin. Further characterization allowed the identification of chrysobactin in diseased tissues, thus providing the first evidence for the externa1 release of a microbial siderophore during pathogenesis. Competition for nutritional iron was also studied through a plant-bacterial cell system: iron incorporated into plant ferritin appeared to be considerably reduced in bacteria-treated suspension soybean cells. l h e same effect was visualized during treatment of soybean cells with axenic leaf intercellular fluid from E. chrysanthemi-inoculated saintpaulia leaves or with chrysobactin.

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In vitro transformation of cultured cells from Nicotiana tabacum by Agrobacterium tumefaciens

Cited by 248 publications

References 13 publications

Efficient octopine Ti plasmid-derived vectors forAgrobacterium-mediated gene transfer to plants

Efficient octopine Ti plasmid-derived vectors forAgrobacterium-mediated gene transfer to plants

Construction of a heat‐inducible chimaeric gene to increase the cytokinin content in transgenic plant tissue

Iron Deficiency Induced by Chrysobactin in Saintpaulia Leaves Inoculated with Erwinia chrysanthemi

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