In vitro toxicity screening of polyphyto combination using Caco-2-cell line

Upadhayaya, Kumud; Madhav, N. V. Stheesh; Abhishek, B.

doi:10.5897/ajpp11.626

Cited by 1 publication

(1 citation statement)

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“…Fungal immunomodulatory protein (FIP) is a species of small molecular weight protein derived from some medicinal and edible mushrooms, which shares considerable structural and functional similarity (Li, Wang, & Zhou, 2011;Wang et al, 2012). The structure of FIPs is similar to phytohemagglutinin and immunoglobulin, and the wild-type FIPs exist as homodimers (Williams & Barclay, 1988;Xu et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Characterization of a novel fungal immunomodulatory protein, FIP-SJ75 shuffled from Ganoderma lucidum , Flammulina velutipes and Volvariella volvacea

Shao

Mao

et al. 2019

Food and Agricultural Immunology

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FIP-SJ75, a novel fungal immunomodulatory protein gene, was shuffled from the genes of three different mushroom species: Ganoderma lucidum, Flammulina velutipes, and Volvariella volvacea. Based on the expression of FIP-SJ75 gene in Escherichia coli, recombinant FIP-SJ75 (rFIP-SJ75) was routinely confirmed, consisting of 115 amino acids, and six peptides were identified by LC/Q-TOF MS with a coverage rate of 84.3%. Bioactivity assay in vitro indicated that rFIP-SJ75 promoted the proliferation of RAW264.7 cells at a range of 1-8 μg/mL, and significantly activated RAW264.7 cells in a dose-dependent manner. Real-time PCR revealed rFIP-SJ75 obviously promoted pro-inflammatory genes (TNF-α and IL-6), but reduced the expression of anti-inflammatory genes (IL-10 and TGF-β1) at the transcriptional level. These data implied that rFIP-SJ75 possessed immunomodulatory activity in macrophages by promoting macrophage M1 polarization and initiating pro-inflammatory responses, which could lay the foundation for rFIP-SJ75 to become a stable immunomodulator resource for further research and potential applications.

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