In vitro toxicity of nanoparticles in BRL 3A rat liver cells

Hussain, Saber M.; Hess, Krista L.; Gearhart, Jeffery M.; Geiss, Kevin T.; Schlager, John J.

doi:10.1016/j.tiv.2005.06.034

Cited by 1,840 publications

(1,183 citation statements)

References 17 publications

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“…It has been reported that kinematic parameters of sperm was progressively reduced by higher concentrations of nanosilver in vitro (Mirshokraei et al, 2011). Following exposure to silver nanoparticles, liver, neuroendocrine and germ cell lines have shown impaired function of mitochondria (Braydich-Stolle et al, 2005;Hussain et al, 2005;Hussain et al, 2006). This characteristic of response was similar in all cells.…”

Section: Discussionmentioning

confidence: 79%

In vitro effect of nanosilver on gene expression of superoxide dismutases and nitric oxide synthases in chicken sertoli cells

Hassanpour

Mirshokraei

Sadrabad

et al. 2015

Animal

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To evaluate effects of different concentrations of nanosilver colloid on the cell culture of Sertoli cells, the proportion of lipid peroxidation, antioxidant capacity, nitric oxide (NO) production and genes expression of superoxide dismutases (SOD1 and SOD2) and nitric oxide synthases (eNOS and iNOS) were measured. Sertoli cells were incubated at concentrations of 25, 75 and 125 ppm nanosilver for 48 h. There was progressive lipid peroxidation in treatments according to increasing of nanosilver. Lipid peroxidation, as indicated by malondialdehyde levels, was significantly elevated by the highest concentration of silver colloid (125 ppm), although antioxidant capacity, as measured by ferric ion reduction, was unaffected. Nitrite, as an index of NO production was reduced only in 125 ppm of nanosilver. Expression of SOD1 gene was reduced in nanosilver-treated cells at all concentrations, whereas expression of SOD2 gene was reduced only in cells treated with 125 ppm nanosilver. Expression of iNOS gene was progressively increased with higher concentrations of nanosilver. Expression of eNOS gene was also increased in 125 ppm of nanosilver. In conclusion, toxic effects of nanosilver could be due to high lipid peroxidation and suppression of antioxidant mechanisms via reduced expression of SOD genes and increased expression of NOS genes.

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Section: Discussionmentioning

confidence: 79%

In vitro effect of nanosilver on gene expression of superoxide dismutases and nitric oxide synthases in chicken sertoli cells

Hassanpour

Mirshokraei

Sadrabad

et al. 2015

Animal

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“…However, the use of animal models, as well as various treatment methods and nanoparticles with different concentrations and shapes, presents new horizons for further research to investigate the applications of nanotechnology (46). Some studies have emphasized the toxicity of nanoparticles in in vivo conditions, considering the molecular effects of some nanoparticles with different concentrations and shapes in the tissues of laboratory animals prior to use on humans (47).…”

Section: Discussionmentioning

confidence: 99%

Bactericidal Activity of Copper Oxide Nanocomposite/Bioglass for in Vitro Clindamycin Release in Implant Infections Due to Staphylococcus aureus

Alijanian¹,

Talebian²,

Doudi³

2016

Avicenna J Med Biochem

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Background: In recent years, bioactive bioceramics such as bioglass and hydroxyapatite (HA) have been introduced as a remarkable development in the field of medicine due to their bio-adaptability, non-toxicity, and persistence, in vivo. They have many potential applications in the repair of bone defects and hence they have attracted significant interest from scholars. Objectives: The aim of this study was to synthesize inorganic matrix CuO-based bioglasses and evaluate their antibacterial activity against aerobic bacterial infections in bone implants. Methods: Nano-composite samples of silica-based bioactive glass, 60S BG with nano-powder CuO, were synthesized using the sol-gel method and then assessed with regard to their antibacterial properties against Staphylococcus aureus using well diffusion agar. The samples included BG58S (58%SiO2, 36%CaO, 6%P2O5), BG/10CuO (58%SiO2, 26%CaO, 6%P2O5, 10%CuO), and BG/20CuO (48%SiO2, 26%CaO, 6%P2O5, 20%CuO). To evaluate their bioactivity, the prepared samples of BG/20CuO, BG/10CuO, and BG58S were immersed in simulated body fluids (SBF). The surface morphology and structure of the samples before and after immersion in the SBF were characterized using scanning electron microscopy (SEM) and Fourier transform infrared (FTIR), respectively. Then, the BG/20CuO and BG/10CuO samples were loaded in clindamycin, an antibiotic widely used in the treatment of osteomyelitis, and their release profiles were studied in phosphate buffer solution. Results: It was observed that the growth inhibition zone increased through clindamycin release due to the increasing CuO percentage in the nanocomposite of bioactive glass. The bioactivity of the nanocomposite/bioglass with CuO was superior to that of bioglass alone. In this study, the BG/20CuO sample showed a sustained release of clindamycin, which is sufficient for a drug delivery system. Conclusions: Increasing the Cu nanoparticles in bioactive glass samples leads to the release of Cu 2+ , which has a positive effect on the antibacterial mechanism, as well as decreasing the cultured Staphylococcus colonies found on the bioglasses. Therefore, it seems that the nanocomposite/bioglass of CuO is a promising option for aerobic bacterial inhibitor systems in common bone implant infections.

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“…It has been employed to validate other methods, including when determining nanoparticle toxicity. [52] Results for different types of particles have been reported in the literature, including nanoparticles of titanium dioxide, iron oxide, zinc oxide, and silica [53][54][55] The absorption spectrum of reduced MTT dye is pH dependent, and some metal ions affect the MTT reduction reaction. [56] Hence, nanoparticles could potentially interact with the MTT substrate e.g.…”

Section: Mtt Interference Assaymentioning

confidence: 99%

Effect of Modification Protocols on the Effectiveness of Gold Nanoparticles as Drug Delivery Vehicles for Killing of Breast Cancer Cells

et al. 2016

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The current study evaluated the potential of gold nanoparticles (AuNPs) for the delivery of Taxol to breast cancer cells (T47D) using an in vitro cell culture model. For this study, new loading approaches and novel chemical attachments were investigated. Five different gold nanoparticle-based complexes were used to determine their cytotoxicity towards T47D cells using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) viability assay. There was no significant decrease (P . 0.05) in cell viability when T47D cells were treated with AuNPs that did not contain Taxol. However, cells were significantly killed by gold nanoparticles chemically conjugated to Taxol using three different approaches and one novel hybrid AuNP-Taxol nanoparticle, wherein no chemical bonds were involved. These Taxolloaded AuNPs were more effective at inducing cell death in vitro than a solution of free Taxol used to treat cells. This result demonstrated that Taxol could be released from the particles in the cell culture media for subsequent therapeutic action. Additionally, the experiments proved that the Taxol-loaded AuNPs were more toxic in a dose dependent manner than Taxol as a formulation for the treatment of breast cancer cells. The results of this study suggest that gold nanoparticles have potential for the efficient delivery of Taxol to breast cancer cells. This could provide a future solution as an alternative application method to overcome adverse side effects resulting from current high-dose treatment regimes.

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In vitro toxicity of nanoparticles in BRL 3A rat liver cells

Cited by 1,840 publications

References 17 publications

In vitro effect of nanosilver on gene expression of superoxide dismutases and nitric oxide synthases in chicken sertoli cells

In vitro effect of nanosilver on gene expression of superoxide dismutases and nitric oxide synthases in chicken sertoli cells

Bactericidal Activity of Copper Oxide Nanocomposite/Bioglass for in Vitro Clindamycin Release in Implant Infections Due to Staphylococcus aureus

Effect of Modification Protocols on the Effectiveness of Gold Nanoparticles as Drug Delivery Vehicles for Killing of Breast Cancer Cells

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