In vitro toxicity of local anaesthetics and corticosteroids on supraspinatus tenocyte viability and metabolism

Nuelle, Clayton W.; Cook, Cristi R.; Stoker, Aaron M.; Cook, James L.; Sherman, Seth L.

doi:10.1016/j.jot.2016.08.002

Cited by 18 publications

(20 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the same steroid dose in uninjured tendons initiates a short-term response equivalent to that of structural injury. Nuelle et al 32 Y Peritendinous injection of lidocaine, betamethasone, and methylprednisolone results in significant supraspinatus tenotoxicity in vitro. Ghellioni et al 33 Y Y There is a dose-dependent reduction of mechanical resistance and histological modifications of rotator cuff tendon exposed to corticosteroids.…”

Section: Systematic Review and Study Inclusionmentioning

confidence: 99%

Adverse Impact of Corticosteroids on Rotator Cuff Tendon Health and Repair: A Systematic Review of Basic Science Studies

Puzzitiello

Patel

Forlenza

et al. 2020

Arthroscopy, Sports Medicine, and Rehabilitation

View full text Add to dashboard Cite

Purpose: To evaluate the in vitro effects of corticosteroid injections (CSIs) on rotator cuff tendon (RCT). Methods: A systematic review of the MEDLINE database was performed according to Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines for all studies reporting on adverse biochemical and biomechanical effects of CSIs on RCT. Results: Sixteen studies were identified that had been published in the last 15 years on the effects of corticosteroids on RCTs. Eight of these studies were on human RCTs, 6 were on rat tendons, 1 considered both human and rat tendons, and 1 was on dog tendon. Five studies analyzed the effects of corticosteroids on the biomechanical properties of RCT or rotator cuff repair, whereas the remaining observed the cellular and molecular effects of CSIs on RCT. Corticosteroids suppress an inflammatory response, induce apoptosis, and have negative effects on collagen and tendon cell viability in RCTs. The mechanical properties, including load to failure of RCTs and rotator cuff repair anchor pull-out strength, also are decreased by CSIs. These in vitro effects appear to be transient as well as frequency and dose dependent. Conclusions: On a molecular level, CSIs decrease cellular proliferation, alter collagen and extracellular matrix composition, impede inflammatory pathways, decrease cellular viability, increase adipocyte differentiation, and increase apoptosis. These changes can be seen as early as 24 hours after corticosteroid exposure, last as long as 2 to 3 weeks, and are exacerbated by increased doses and decreased latency between doses. Biomechanical studies demonstrate that these changes result in decreased maximal load to failure, tendon stiffness, and suture anchor pull-out strength in rat shoulders up to 2 weeks but not at 3 and 4 weeks, post-CSI. Clinical Relevance: Shoulder subacromial steroid injection is common, and practitioners should be aware of results both positive and deleterious.R otator cuff tendon (RCT) pathology is the most common cause of shoulder pain. 1 Rotator cuff tendinosis, which refers to processes such as impingement, rotator cuff fraying, partial-thickness tears, and tendinitis, is a normal element of human aging. 1,2 While often asymptomatic, RCT pathology can be a source of pain and significantly impact patients' quality of life. 3 Corticosteroid injections (CSIs) into the subacromial space have served as a valuable treatment modality to the physician treating patients with RCT pathology refractory to conservative management, as they have demonstrated to improve symptoms in the short term. 3e6 However, CSIs have failed to consistently provide long-term pain relief, and recent studies suggest that CSIs may even predispose to worse longterm outcomes, such as pathologic progression or surgical failure. 4,5,7e9

show abstract

Section: Systematic Review and Study Inclusionmentioning

confidence: 99%

Adverse Impact of Corticosteroids on Rotator Cuff Tendon Health and Repair: A Systematic Review of Basic Science Studies

Puzzitiello

Patel

Forlenza

et al. 2020

Arthroscopy, Sports Medicine, and Rehabilitation

View full text Add to dashboard Cite

show abstract

“…Since the above mentioned molecular probes almost cover the entire visible spectrum, to gate living cells in low cytometry one needs to use two corresponding dyes -7-AAD and Sytox Blue. The use of these dyes makes it possible to carry out a multiparametric analysis of the state of cells and, without any significant interference, to study up to 4 parameters simultaneously in the same cell [15]. Some of the listed parameters can be analyzed not only by flow cytometry, followed by quantitative data, but also by fluorescence microscopy.…”

Section: Assessment Of the Effect Of Probiotics On Intracellular Processes Using Flow Cytometry And Microscopymentioning

confidence: 99%

“…Lysotracker yellow [8] Mitochondrial function Rhodamine 123, MitoTracker Red [9], [17] The content of phospholipids and triglycerides Nile Red [10] The glucose uptake 6-NBDG [11] Proliferation rate CellTrace CSFE [12] ROS level CellRox Orange, CellRox Green [13] Cellular thiols ThiolTracker Violet [14] Viability 7-AAD, Sytox Blue [15] The peroxisomal system function CellLight Peroxisome-GFP [18] Autophagy intensity LysoTracker Yellow, immunostaning against MAP1LC3B [19] Amino acids import rate Immunostaning against SLC3A2 [20]…”

Section: Lysosomal Functionmentioning

confidence: 99%

Approaches to probiotics properties testing using Caco-2 cells

et al. 2020

View full text Add to dashboard Cite

Adequate biological models are a prerequisite to screening and development of probiotic drugs. In the present study, Caco-2 cell line is reviewed as a model for analyzing properties of probiotics. This cell culture possesses all the characteristics necessary for evaluating the effects of probiotic drugs on a wide range of both intracellular processes and intercellular interactions. Informativeness of Caco-2 cells is in the focus of the present review.

show abstract

“…During arthroscopic joint surgery, intra-articular administration of LAs is common to improve postoperative pain scores and reduce narcotic consumption [1,2]. LAs are also administered into the shoulder joint to treat rotator cuff injuries and diseases [5]. In addition, fat aspiration for a cosmetic purpose or isolation of autologous adipose-derived stem/progenitor cells (ADSCs) routinely involves an administration of LAs [6][7][8][9][10].…”

Section: Introductionmentioning

confidence: 99%

Effect of local anesthetics on viability and differentiation of various adult stem/progenitor cells

et al. 2020

View full text Add to dashboard Cite

Background Local anesthetics (LAs) are widely used to control pain during various clinical treatments. One of the side effects of LAs, cytotoxicity, has been investigated in various cells including stem/progenitor cells. However, our understanding of the effects of LAs on the differentiation capacity of stem/progenitor cells still remains limited. Therefore, a comparative study was conducted to investigate the effects of multiple LAs on viability and multi-lineage differentiation of stem/progenitor cells that originated from various adult tissues. Method Multiple types of stem/progenitor cells, including bone marrow mesenchymal stem/progenitor cells (MSCs), dental pulp stem/progenitor cells (DPSCs), periodontal ligament stem/progenitor cells (PDLSCs), and tendon-derived stem/progenitor cells, were either obtained from a commercial provider or isolated from adult human donors. Lidocaine (LD) and bupivacaine (BP) at various doses (1×, 0.75×, 0.5×, and 0.25× of each physiological dose) were applied to the different stem/progenitor cells for an hour, followed by induction of fibrogenic, chondrogenic, osteogenic, and adipogenic differentiation. Live/dead and MTT assays were performed at 24 h after the LD or BP treatment. At 2 weeks, qRT-PCR was conducted to evaluate the gene expressions associated with differentiation. After 4 weeks, multiple biochemical staining was performed to evaluate matrix deposition. Results At 24 h after LD or BP treatment, 1× and 0.75× physiological doses of LD and BP showed significant cytotoxicity in all the tested adult stem/progenitor cells. At 0.5×, BP resulted in higher viability than the same dose LD, with variance between cell types. Overall, the gene expressions associated with fibrogenic, chondrogenic, osteogenic, and adipogenic differentiation were attenuated in LD or BP pre-treated stem/progenitor cells, with notable dose-effect and dependence on types. In contrast, certain doses of LD and/or BP were found to increase specific gene expression, depending on the cell types. Conclusion Our data suggest that LAs such as LD and BP affect not only the viability but also the differentiation capacity of adult stem/progenitor cells from various anatomical sites. This study sheds light on stem cell applications for tissue regeneration in which isolation and transplantation of stem cells frequently involve LA administration.

show abstract

In vitro toxicity of local anaesthetics and corticosteroids on supraspinatus tenocyte viability and metabolism

Cited by 18 publications

References 24 publications

Adverse Impact of Corticosteroids on Rotator Cuff Tendon Health and Repair: A Systematic Review of Basic Science Studies

Adverse Impact of Corticosteroids on Rotator Cuff Tendon Health and Repair: A Systematic Review of Basic Science Studies

Approaches to probiotics properties testing using Caco-2 cells

Effect of local anesthetics on viability and differentiation of various adult stem/progenitor cells

Contact Info

Product

Resources

About