In vitro toxicity and genotoxic activity of aqueous leaf and fruit extracts of Ruscus hypophyllum L.

Verschaeve, Luc; Edziri, Hayet; Anthonissen, Roel; Boujnah, Dalenda; Harzallah‐Skhiri, Fethia; Aouni, Mahjoub; Mastouri, Maha

doi:10.1007/s11738-017-2505-7

Cited by 3 publications

(1 citation statement)

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“…Single phenolic compounds, such as catechin and gallic acid, also induced DNA damage. Verschaeve et al [52] demonstrated the genotoxicity of Ruscus hypophyllum fruit and leaf extracts against human C3A hepatic cells. DNA damage was detected at the highest extract concentration studied of 5 mg/mL.…”

Section: Discussionmentioning

confidence: 99%

Cytotoxic and DNA-Damaging Effects of Aronia melanocarpa, Cornus mas, and Chaenomeles superba Leaf Extracts on the Human Colon Adenocarcinoma Cell Line Caco-2

Efenberger-Szmechtyk

Nowak

2020

Antioxidants

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Aronia melanocarpa, Cornus mas, and Chaenomeles superba leaf extracts contain large amounts of bioactive compounds—mainly polyphenols, which possess many health benefits including anti-cancer properties. Here, we investigate the biological effects of A. melanocarpa, C. mas, and C. superba leaf extracts on the human colon adenocarcinoma cell line Caco-2. The antiproliferative activity of the extracts was measured using the MTT assay. The most cytotoxic extract was C. mas (IC50 = 0.60%). The extracts caused morphological changes in the Caco-2 cells, including partial detachment of cells, necrotic cells, chromatin condensation, cytoplasmic vacuolization, cell nuclei lysis, and nucleus fragmentation. The DNA damage in the Caco-2 cells after exposure to the leaf extracts was measured using the alkaline comet assay. The extracts increased DNA damage in a concentration dependent manner. However, at lower non-cyto- and non-genotoxic (IC0) concentrations the extracts induced DNA repair in Caco-2 cells after exposure to hydrogen peroxide. In conclusion, the results of these studies suggest that A. melanocarpa, C. mas and C. superba leaf extracts can show anticancer activity. However, further research is required on the mechanisms of anti-cancer activity by these extracts, with the application of more advanced and wide-ranging techniques including in vivo experiments.

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Section: Discussionmentioning

confidence: 99%