In Vitro: The Effects of the Anticoccidial Activities of Calotropis procera Leaf Extracts on Eimeria stiedae Oocysts Isolated from Rabbits

Murshed, Mutee; Aljawdah, Hossam M.A.; Mares, Mohammed M.; Al‐Quraishy, Saleh

doi:10.3390/molecules28083352

Cited by 5 publications

(1 citation statement)

References 54 publications

(52 reference statements)

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“…Our findings are better than those reported for pulp and leaf olive extracts (25.36% and 5.87%, respectively) [22,60], whereas 60.53 ± 0.38 mg/mL of OFI peel extract destroyed 50 oocysts (Figure 5), which is higher than the reported values of Debbou-Iouknane, Nerín, Amrane-Abider, and Ayad [22], where 192.94 mg/mL of olive leaves destroyed half of the Eimeria oocysts isolated from broiler chickens. Moreover, while the present results were better even against Eimiria isolated from other animals like rabbits, Murshed et al [61] revealed that the oocyst inhibition percentages were low (below 50%) using 25-100 mg/mL of Calotropis procera leaf.…”

Section: Anticoccidial Activitiesmentioning

confidence: 46%

Opuntia Ficus-Indica Peel By-Product as a Natural Antioxidant Food Additive and Natural Anticoccidial Drug

Amrane-Abider,

Imre,

Herman

et al. 2023

Foods

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The present study was carried out to valorize the Opuntia ficus-indica (OFI) by-products by extracting and identifying their biochemical compounds and evaluating their antioxidant potential by in vitro activities (DPPH radical and FRAP), as well as their capacity to stabilize margarine oxidation (rancimat test). In addition, their in vitro anticoccidial effect on the destruction of Eimeria oocysts isolated from naturally infected chickens was also targeted. Microwaves and response surface methodology tools were used to extract the maximum amount of phenolic compounds (42.05 ± 0.46 GAE mg/g DW of total phenolic compounds in 90 s at 400 watts). Moreover, the effect of extraction factors was also studied. Eight phenolic compounds, including isorhamnetin, dihydrokaempferol, and kaempferol-3-O-rutinoside, were identified. The findings confirmed that OFI peel extract has strong antioxidant activities (DPPH radical, ferric reducing power). The rancimat test shows that OFI peel extract improves margarine stability by 3.2 h. Moreover, it has a notable destruction rate of Eimeria oocysts (30.06 ± 0.51%, LC50: 60.53 ± 0.38 mg/mL). The present investigation offers promise for the reuse of food waste as natural margarine additives, protection of the environment, and substitution of anticoccidial synthetic treatments.

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Section: Anticoccidial Activitiesmentioning

confidence: 46%

Opuntia Ficus-Indica Peel By-Product as a Natural Antioxidant Food Additive and Natural Anticoccidial Drug

Amrane-Abider,

Imre,

Herman

et al. 2023

Foods

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Therapeutic efficacy of Citrus aurantifolia (lime) juice in experimental Eimeria tenella–infected broiler chickens

Ugwuoke,

Obi,

Onu

et al. 2023

Trop Anim Health Prod

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The in vivo anticoccidial activity of Citrus aurantifolia juice (CAJ) against Eimeria tenella-infected broilers was evaluated in an effort to obtain scientific information for usage by the natives in Nigeria. C. 2 aurantifolia fruits were collected, identified, cut transversely using a sterile knife and the lime juice extracted and stored airtight for further experiment in a refrigerator to reduce oxidation and fermentation.A total of 72, three-week-old experimentally E. tenella-infected broilers consisting of 6 groups, n = 12 (4 replicates with 3 birds each) were orally treated in drinking water with graded doses of (Group A -20 ml/l, 10 ml/l for B, and 5 ml/l for C) of C. aurantifolia juice in the 7-day oocyst reduction and curative tests study. Group D -Amprolium (1.5 mg/ml) and group E -(infected/untreated) were used as positive and negative controls respectively while group F served as normal control. Results showed that CAJ highly suppressed the parasites irrespective of the dose, decreased oocyst per gram of faeces (OPG), enhanced caecal lesion and caused statistically significant (P < 0.05) rise in PCV content. Amproliumtreated broilers (Group D) was more potent in OPG reduction to 290 ± 0.22 oocysts by day 6. Group-A had an OPG drop to 1000 ± 0.06 oocysts on day 8 following therapy, in contrast to infected/untreated which recorded 2377 ± 13.0 oocysts on day 12 post treatment. Citrus aurantifolia ameliorated the effects of Coccidiosis caused by E. tenella in broiler chickens.

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The Anticoccidial In Vitro Effects and Antioxidant Properties of Several Plants Traditionally Used for Coccidiosis in Togo

Tchodo,

Dakpogan,

Sanvee

et al. 2024

Veterinary Sciences

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Coccidiosis is a parasitic disease that often affects livestock. Identifying plants with inhibitory effects on the development of the parasite could help in finding new natural treatments. This study aimed to evaluate the anticoccidial potentials of extracts from Azadirachta indica leaves (AILs), Combretum micranthum leaves (CMLs), Carica papaya seeds (CPSs), Sarcocephalus latifolius roots (SLRs), and Vernonia amygdalina leaves (VALs). The in vitro anticoccidial efficacy of the extracts was evaluated through oocyst sporulation inhibition and sporozoite viability inhibition assays of Eimeria oocysts. The setup was examined for 72 h (every 24 h) of incubation. The DPPH radical scavenging activity and ferric reducing antioxidant power were used to evaluate the antioxidant potential of the extracts. Among the tested extracts, the SLR, CPS, and AIL extracts exhibited the maximum oocyst sporulation inhibition (75.85 ± 1.21%, 74.53 ± 1.65%, and 71.58 ± 0.24%, respectively) at a concentration of 75 mg/mL of plant extracts against the Eimeria species. The Sarcocephalus latifolius root extract showed the highest radical scavenging capacity (76.25 ± 0.53) and reducing power (86.21 ± 4.28). The biochemical screening of the selected plant extracts revealed the presence of antioxidant compounds such as phenols, flavonoids, alkaloids, saponins, and carbohydrates. The SLR extract contained the highest amounts of phenols (56.11 ± 0.33 µg/mL) and flavonoids (36.65 ± 1.85 µg/mL). In conclusion, the selected hydro-ethanolic extracts from these plants possess excellent anticoccidial and antioxidant activities, which can be attributed to the presence of medicinally important phytochemicals. Further research is needed to identify and isolate the active anticoccidial compounds from these plants, which could be utilized in the development of drugs against coccidiosis.

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In Vitro: The Effects of the Anticoccidial Activities of Calotropis procera Leaf Extracts on Eimeria stiedae Oocysts Isolated from Rabbits

Cited by 5 publications

References 54 publications

Opuntia Ficus-Indica Peel By-Product as a Natural Antioxidant Food Additive and Natural Anticoccidial Drug

Opuntia Ficus-Indica Peel By-Product as a Natural Antioxidant Food Additive and Natural Anticoccidial Drug

Therapeutic efficacy of Citrus aurantifolia (lime) juice in experimental Eimeria tenella–infected broiler chickens

The Anticoccidial In Vitro Effects and Antioxidant Properties of Several Plants Traditionally Used for Coccidiosis in Togo

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