Acaricidal effects of fipronil against Rhipicephalus (Boophilus) annulatus were studied in vitro using adult immersion test. A concentration-dependent increase in adult mortality was observed. At a concentration of 1 ppm, there was 100% inhibition of fecundity. Complete blocking of hatching of the laid ova was observed even at 500 ppb. Based on probit analysis, the 50% lethal concentration value was calculated as 12.78 ppm, which was much higher than the previously reported values against Rhipicephalus (Boophilus) microplus.