In Vitro Tests for a Rapid Evaluation of Antidiabetic Potential of Plant Species Containing Caffeic Acid Derivatives: A Validation by Two Well-Known Antidiabetic Plants, Ocimum gratissimum L. Leaf and Musanga cecropioides R. Br. ex Tedlie (Mu) Stem Bark

Awwad, Abdulmomem; Poucheret, Patrick; Idres, Yanis A.; Tshibangu, Damien S. T.; Servent, Adrien; Ferrare, Karine; Lazennec, Françoise; Bidel, Luc; Cazals, Guillaume; Tousch, Didier

doi:10.3390/molecules26185566

Cited by 8 publications

(6 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus if the major finding of this research is translatable to human, extract of Ocimum gratissimum may be beneficial as a nutritional supplement in the prevention of aspartame induced renal insufficiency. Although the beneficial effects of Ocimum gratissimum extract in some human ailments using animal models have been documented in chemical liver injury [25], diabetes mellitus [26], bacterial infections [27] and human cancer cell lines [28][29][30] but not in relation to aspartame and chronic kidney disease. The outcome of this study is the first documented evidence of the beneficent of O. gratissimum in aspartame induced kidney insufficiency.…”

Section: Discussionmentioning

confidence: 99%

Aspartame, Chronic Kidney Insufficiency and Ocimum gratissimum Extract

Ajani,

Agagwu

2023

JAMPS

View full text Add to dashboard Cite

Objective: The paired human kidneys are retroperitoneal organs responsible for the maintenance of the internal meliu of the human body. Chronic kidney disease is major cause of morbidity and mortality globally. Nutrition and life style are two main factors in the aetiopathogenesis of chronic kidney disease. Aspartame is a non-nutritive sweetener commonly consumed worldwide. Ocimum gratissimum is a naturally growing plant with some medicinal benefits. This study investigated if oral administration of aspartame could induce renal insufficiency in rat and the plausible ameliorative role of O. gratissimum extract. Methodology: Fifty animals allotted to four groups were used for the study. The control group (CN) had normal feed. The Aspartame group (Asp) had 250 mg/kg/day of aspartame for 28 days. The Aspartame low dose O. gratissimum extract group (ALO) had 250 mg/kg/day of aspartame for 28 days followed by once daily dose of the extract at 100 mg/kg for 28 days. For the Aspartame high dose O. gratissimum extract group (AHO); aspartame was administered at 250 mg/kg once daily for 28 days followed by another 28-day daily administration of the plant extract at 200mg/kg. Both aspartame and plant extract were administered orally. Periodically, blood samples were collected for biochemical analyses and kidneys harvested for histopathological examination. Results: The biochemical parameters of renal insufficiency were significantly pronounced in the Asp group but not in the AHO. Oxidative stress was pronounced in the Asp and ALO but not in group AHO at day 57. Histopathological examination of the kidney obtained from the Aspartame group revealed destruction of glomeruli. Chronic administration of aspartame in rat caused sustained renal insufficiency which was ameliorated by prolonged administration of high dose of Ocimum gratissimum extract. Thus O. gratissimum extract is beneficial to aspartame induced renal toxicity in a dose dependent manner.

show abstract

Section: Discussionmentioning

confidence: 99%

Aspartame, Chronic Kidney Insufficiency and Ocimum gratissimum Extract

Ajani,

Agagwu

2023

JAMPS

View full text Add to dashboard Cite

show abstract

“…Interestingly, both extracts and the EO were sources of polyphenols. For example, the measured total phenolic content in Z. tenuior was equal to 49.0 ± 1.4 mg mg gallic acid/100 g of EO [15].…”

Section: Chemical Composition Of Extracts By Hplc-esi-qtof-ms/msmentioning

confidence: 97%

“…Both Ziziphora species were also rich in phenolic compounds. These authors investigated the antibacterial activity of EOs against important foodborne pathogenic bacteria and showed that they could be considered as good sources of natural antibacterial material as well as food preservative [2,15].…”

Section: Candida Glabratamentioning

confidence: 99%

Multidirectional Characterization of Phytochemical Profile and Health-Promoting Effects of Ziziphora bungeana Juz. Extracts

et al. 2022

View full text Add to dashboard Cite

Ziziphora species (Lamiaceae) have been used in traditional medicine as sedatives, antiseptics, carminatives, or expectorants. Despite their common applications in phytotherapy, there is still lack of evidence about the composition of their extracts and its impact on biological properties of the plants. The aim of this study was to evaluate the content of Ziziphora bungeana, a less studied species growing in Kazakhstan, using HPLC-ESI-QTOF-MS/MS instrumentation and to determine its antimicrobial, antioxidant, and cytotoxic activity together with inhibitory properties against tyrosinase and toxicity in erythrocyte lysis assay. Extracts from Z. bungeana were found to be sources of flavonoids, phenolic acids, organic acids, and terpenes that determined their antiradical activity. The minimum inhibitory concentrations of extracts were lower for Gram-positive bacteria (1.25–10 mg/mL) than for Gram-negative bacteria and fungi (5–20 mg/mL). The EC50 value calculated for antiradical activity ranged between 15.00 ± 1.06 µg/mL and 13.21 ± 3.24 µg/mL for ABTS and DPPH assays, respectively. Z. bungeana extracts were found to decrease the activity of tyrosinase by 50% (at 200 µg/mL) similarly to kojic acid and were slightly cytotoxic for human melanoma A375 cell line (at 200 µg/mL) with no effect on HaCaT keratinocytes. In the end, Z. bungeana did not reveal toxic effects in hemolytic assay as compared to the positive control Triton X-100. The performed tests show potential application of the plant in the treatment of infectious diseases, disorders caused by free radicals, and skin problems.

show abstract

“…"A recent study revealed the ethanolic leaf extract of M. cecropioides to possess both anti-inflammatory and anti-nociceptive effect. In vitro and in vivo hypoglycemic properties of the leaf and stem bark have also been reported" [16]. Since imbalance in oxidative stress has been implicated in the complications of diabetes and multiple organ failure from chemical/xenobiotics toxicities, this study investigated the effect of the leaf extract and fractions of M. cecropioides on both chemical and diabetes induced oxidative stress.…”

Section: Musanga Cecropioidesmentioning

confidence: 99%

Musanga cecropioides Attenuates Chemical and Diabetes Induced Oxidative Stress in Experimental Animal Models

Ogbue

Onyegbule

Ezugwu

et al. 2022

JSRR

View full text Add to dashboard Cite

Oxidative stress plays an important role in both induction of diabetes and development of diabetes complications. This study evaluated the effect of leaf extract and fractions of M. cecropioides on both chemical and disease induced oxidative stress. The leaves were extracted with methanol and partitioned into various fractions. Phytochemical analysis and total phenolic content (TPC) were done for the extract and fractions. The DPPH (2,2-diphenyl-1-picrylhydrazyl) and Ferric reducing antioxidant power assay were used to evaluate their in vitro antioxidant potentials of the extracts and fractions at 52.5, 125, 250, 500 and 1000 µg/ml concentrations with ascorbic acid as reference standard. Carbon tetrachloride and streptozotocin-Nicotinamide (STZ-NAD) were used to induce chemical and diabetes induced oxidative stress respectively. Treatments were done with 200 and 400 mg/kg of the extract and fractions using Silymarin as reference standard. Liver function tests and lipid peroxidation (LPO) were used to monitor CCL4 induced oxidative stress while serum antioxidant enzymes (catalase and superoxide dismutase), LPO and total plasma antioxidant assays were used to monitor diabetes induced oxidative stress. The ethyl acetate fraction (EAF) showed the highest TPC of 386.06 mgGAE/g. The EAF and butanol fraction (BF) showed better DPPH activity compared to the extract with IC50s of 49.78, 71.57, 98.92 µg/ml respectively. The EAF both at 200 and 400 mg/kg maintained non-significant (p > 0.05) difference between pre-induction and post-induction ALT and AST concentrations. Also, only EAF at 400 mg/kg protected against significant rise in CCL4 induced LPO from baseline values which was not achieved with 100 mg/kg silymarin (reference standard treatment). The EAF at 400 mg/kg was the only treatment that produced significant (P<0.05) reduction in blood glucose 24 h post-treatment compared to vehicle control group. The EAF and BF at 400 mg/kg just like 200 mg/kg metformin were able to restore serum catalase almost to their pre-induction values with no significant (P>0.05) difference. M. cecropioides proved effective in both chemical and disease induced oxidative stress.

show abstract

In Vitro Tests for a Rapid Evaluation of Antidiabetic Potential of Plant Species Containing Caffeic Acid Derivatives: A Validation by Two Well-Known Antidiabetic Plants, Ocimum gratissimum L. Leaf and Musanga cecropioides R. Br. ex Tedlie (Mu) Stem Bark

Cited by 8 publications

References 39 publications

Aspartame, Chronic Kidney Insufficiency and Ocimum gratissimum Extract

Aspartame, Chronic Kidney Insufficiency and Ocimum gratissimum Extract

Multidirectional Characterization of Phytochemical Profile and Health-Promoting Effects of Ziziphora bungeana Juz. Extracts

Musanga cecropioides Attenuates Chemical and Diabetes Induced Oxidative Stress in Experimental Animal Models

Contact Info

Product

Resources

About