In-vitro Techniques and Mutagenesis for the Improvement of Vegetatively Propagated Plants

Ahloowalia, B. S.

doi:10.1007/978-94-015-9125-6_15

Cited by 47 publications

(30 citation statements)

References 52 publications

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“…A high frequency of chimeras is often associated with colchicine treatment (Ackerman and Derman 1972;Schifino and Fernandes 1987), but this study indicated that chimeras can be separated by selection from vegetatively propagating axillary shoots. It is often referred to as 'dissolving a chimaera to obtain homo-histonts' and is usually achieved after three or four subcultures (Ahloowalia 1998). Tetraploid and diploid shoots proliferated and rooted (Fig.…”

Section: Resultsmentioning

confidence: 99%

In vitro induction of tetraploid plants from a diploid Japanese pear cultivar ( Pyrus pyrifolia N. cv. Hosui)

Kadota

Niimi

2002

Plant Cell Reports

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Tetraploid plants of a Japanese pear cultivar (Pyrus pyrifolia N.) were induced using an in vitro colchicine treatment. Proliferating shoots were transferred to a shoot proliferation medium (SPM) containing 0.1% or 0.01% colchicine, incubated for 1, 2, 4 or 8 days, then transferred to fresh SPM. The ploidy level of the colchicine-treated individuals was analysed by flow cytometry. Four months after colchicine treatment four mixoploids were selected and cultured on SPM for a further 5 months. The ploidy level of the proliferated shoots derived from selected mixoploids was analysed, and five tetraploid shoots were selected. The selected tetraploid shoots were rooted, and potted plants were grown in a greenhouse. The stomata of tetraploid plants were found to be longer than those in diploid plants.

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Section: Resultsmentioning

confidence: 99%

In vitro induction of tetraploid plants from a diploid Japanese pear cultivar ( Pyrus pyrifolia N. cv. Hosui)

Kadota

Niimi

2002

Plant Cell Reports

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“…By taking the advantage of capacity of callus cells that undergo genetic change in culture, many agronomically desirable callus derived plants have been obtained (Larkin and Scowcraft, 1981). Referred to as somaclonal variation, this has shown great potential in obtaining useful agronomic clones (Ahloowalia, 1998;Jain, 2000). Many examples related to different vegetatively propagated species demonstrate that the combination of in vitro culture and mutagenesis is relatively inexpensive, simple, and efficient.…”

Section: In Vitro Mutagenesismentioning

confidence: 99%

Radiation induced in vitro mutagenesis for sugarcane improvement

Patade

Suprasanna

2008

Sugar Tech

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Induced mutations have contributed significantly to plant improvement and more than 2500 mutant varieties have been developed through mutation breeding. Many examples related to different vegetatively propagated species show that the mutation induction can be empowered by in vitro techniques. Sugarcane is one of the most important industrial crops, used as major source of sugar and recently to produce ethanol, an important renewable biofuel source. At BARC, our group has been engaged in employing in vitro culture techniques in the improvement of sugarcane. Different in vitro culture systems have been developed and radiation induced mutagenesis has been undertaken using embryogenic callus cultures of cv. CoC-671 followed by the in vitro selection and field evaluation of the selected lines. Results on radiationinduced mutagenesis combined with in vitro selection have been reviewed. Also the novel approaches of overcoming intrasomatic competition during in vitro mutagenesis and use of using ion beam irradiation have been discussed.

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“…Their method might be more useful for the induction of mutants without chimerism compared with the method of repeated propagation of axillary buds (Donini & Sonnino, 1998). This latter method will normally result in chimeric (periclinal) mutants but chimerism might cause problems when studying tuber properties during screening (Ahloowalia, 1998).…”

Section: Potato Research 43 (2000)mentioning

confidence: 99%

Development of amylose-free (amf) monoploid potatoes as new basic material for mutation breeding in vitro

et al. 2000

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SummaryFor the induction and selection of double mutants with altered starch composition, a uniform (homohistont) amylose-free (amy') monoploid as basic plant is of vital importance. Therefore, new amf monoploids had to be developed via prickle pollination. And 26 monoploids were obtained which were screened in vitro for vigour, leaf size and for the percentage of monoploid cells. The number of monoploid cells was underestimated at about 28%. Seven vigorous monoploids were tested in vitro for tuberization capacity, which varied from 0 to 100%. After regeneration in vitro, 0 to 3.7 adventitious shoots per leaf explant were formed. The tuberization capacity of these shoots varied from 0 to 48. Two amfmonoploids were selected which were vigorous and tuberized well in vitro. They are the basis for the application of an in vitro mutation breeding protocol that should lead to the induction and selection of new starch mutants in potato.

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In-vitro Techniques and Mutagenesis for the Improvement of Vegetatively Propagated Plants

Cited by 47 publications

References 52 publications

In vitro induction of tetraploid plants from a diploid Japanese pear cultivar ( Pyrus pyrifolia N. cv. Hosui)

In vitro induction of tetraploid plants from a diploid Japanese pear cultivar ( Pyrus pyrifolia N. cv. Hosui)

Radiation induced in vitro mutagenesis for sugarcane improvement

Development of amylose-free (amf) monoploid potatoes as new basic material for mutation breeding in vitro

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