In vitro Teasle Gourd Pollen Germination and Pollen Tube Development as Affected by Sucrose, Boric Acid, and Inorganic Salts

Naik, Abhishek; Akhtar, Shirin; Chattopadhyay, Arup; Thapa, U.; Hazra, P.

doi:10.1080/19315260.2015.1008665

Cited by 7 publications

(5 citation statements)

References 14 publications

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“…Pollen germination is influenced by several factors including nutrition (Imani & Talaie 2006, Bal & Ubak 2005, Maita & Sotomayor 2015, Muengkaew et al 2016 and plant growth regulators (Kovaleva et al 2005, Wu et al 2008. Nutrients, such as sucrose, boron, and magnesium have obvious effects on pollen growth (Chen et al 2009, Liu et al 2013, Naik et al 2016. Sucrose plays an extremely important role and is a significant nutrient for pollen germination (Fei & Nelson 2003, Liu et al 2013.…”

Section: Discussionmentioning

confidence: 99%

“…In vitro pollen germination and tube growth are affected by nutrients and plant growth regulators (Maita et al 2015, Muengkaew et al 2016. Proper application of nutrients and regulators is an effective method to promote pollen germination and tube growth (Lin et al 2017, Naik et al 2016, Radovic et al 2016, and is also beneficial for fruit production (Gao et al 2012). Nevertheless, only a few studies have examined the effects of nutrients and regulators application on the promotion of pollen germination and on the increase of the rate of fruit set of C. oleifera (Gao et al 2012, Tan et al 2010.…”

Section: Introductionmentioning

confidence: 99%

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Orthogonal test design for optimizing culture medium for in vitro pollen germination of interspecific oil tea hybrids

Zhao

Yuan

et al. 2021

An. Acad. Bras. Ciênc.

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Oil Tea (Camellia oleifera) is an important woody edible oil plant in China. Oil Tea suffers from low rate of fruit set during production, which is related to poor pollination and fertilization. Pollen vigor is directly related to pollination and fertilization. Using the interspecifi c hybrid Y3 (C. grijsii × C. oleifera) as plant material, we studied the effects of sucrose, H 3 BO 3 , MgSO 4 , and IAA on pollen germination using an orthogonal design to determine the best culture medium. Results indicated that pollen germination rates were signifi cantly affected by medium components and ranged from 29.13% to 56.84%. Pollen tube length was the longest in the T5 medium surpassing the control group by 489.36 μm. MgSO 4 turned out to be the most important germination medium component having great effect on the pollen germination rate. The optimal culture medium to promote pollen tube growth of Oil Tea Y3 was: 1% agar, 150 g•L-1 sucrose, 0.15 g•L-1 H 3 BO 3 , 0.07 g•L-1 MgSO 4 , and 0.01 g•L-1 IAA. The results of this paper may provide information for foliar application of Mg and IAA, which can improve pollen tube growth of Oil Tea in practice.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Orthogonal test design for optimizing culture medium for in vitro pollen germination of interspecific oil tea hybrids

Zhao

Yuan

et al. 2021

An. Acad. Bras. Ciênc.

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“…4 C and D). The color differentiation between alive and dead pollen using TTC was observed for Jatropha curcas (Naik et al, 2016). The pollen germination rates varied significantly between incubation times and between treatments.…”

Section: Discussionmentioning

confidence: 93%

Pollen storage, viability and effect of growth hormones on in vitro pollen germination in two medicinal plants (Clerodendrum colebrookianum Walp. and Clerodendrum infortunatum L.) of the tropical moist forest of North-east India

Singh

Kumar

Khanduri

2022

JANS

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Clerodendrum colebrookianum Walp. and Clerodendrum infortunatum L. are well known for their medicinal uses in treating various human ailments traditionally. Pollen biology study was done in both plant species to decipher pollen viability, in vitro pollen germination, and pollen storage in varied temperature conditions. Pollen viability tests was done by 2, 3, 5-triphenyl tetrazolium chloride (TTC test) for which pollen grains were collected at the anthesis stage that ascertained high viability, ranged between 71.97%±4.30 % in C. colebrookianum and 81.63%±3.23 in C. infortunatum. In vitro pollen germination was conducted under different hormones (IBA, IAA, GA3, and Kinetin) with concentrations of 100 mg L-1, 200 mg L-1 and 300 mg L-1. Highest germination percentage of 52.10±5.30% and 61.91±1.76% in GA3 (200 mg L-1) was the most suitable growth hormone concentration for inducing in vitro pollen germination in both Clerodendrum species. Statistically, the response of all the concentrations of treatments, sucrose, and hormones, with their time on in vitro pollen germination of C. colebrookianum and C. infortunatum was found significantly different (p<0.05). In contrast, non-significant differences were recorded for in vitro pollen germination between the medicinal plant species. Pollen storage under temperature gradient conditions exhibited a similar trend in the viability for both C. colebrookianum, and C. infortunatum, i.e., the pollen remained viable up to 28 days at -20°C and 6°C, respectively. This study will be helpful in future breeding, hybridization, and conservation efforts for both the Clerodendrum species.

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“…In vitro germination and pollen tube growth of foxtail millet pollen are processes involving multiple factors. In this study, only culture temperature and culture time were compared and analyzed, but different sucrose [52] and boric acid [53] concentrations also affected pollen germination ratio. In addition, pollen germination in vitro was also affected by pH [54] , PEG [55] and BR [56] and other factors.…”

Section: Discussionmentioning

confidence: 99%

Establishment of in vitro pollen germination system in C4 model plant Foxtail Millet

Su,

Wang,

et al. 2024

Plant Cell Tiss Organ Cult

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The pollen development and germination are critical stages for sexual reproduction in plants, this process has great potential application for improve grain yield in crops. In this study, the germination system of foxtail millet pollen was established, and it was found that culture temperature and time were the key factors for pollen germination, among which 22℃ and 30 min may be the best conditions for pollen germination, and the top of the pollen tube would expand if the culture temperature was too high. We also found that the time required for pollen germination of foxtail millet was much shorter than that of other cereals, which provided a new way for us to study the stress resistance of foxtail millet from the perspective of reproductive development. This system may provide signi cant guidance for the application of male sterility or cross breeding.

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In vitro Teasle Gourd Pollen Germination and Pollen Tube Development as Affected by Sucrose, Boric Acid, and Inorganic Salts

Cited by 7 publications

References 14 publications

Orthogonal test design for optimizing culture medium for in vitro pollen germination of interspecific oil tea hybrids

Orthogonal test design for optimizing culture medium for in vitro pollen germination of interspecific oil tea hybrids

Pollen storage, viability and effect of growth hormones on in vitro pollen germination in two medicinal plants (Clerodendrum colebrookianum Walp. and Clerodendrum infortunatum L.) of the tropical moist forest of North-east India

Establishment of in vitro pollen germination system in C4 model plant Foxtail Millet

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