In Vitro Targeting of Avidin-Expressing Glioma Cells with Biotinylated Persistent Luminescence Nanoparticles

Abstract

Far red emitting persistent luminescence nanoparticles (PLNP) were synthesized and functionalized with biotin to study their targeting ability toward biotin-binding proteins. First, the interaction of biotin-decorated PLNP with streptavidin, immobilized on a plate, was shown to be highly dependent on the presence of a PEG spacer between the surface of the nanoparticles and the biotin ligand. Second, interaction between biotin-PEG-PLNP and free neutravidin in solution was confirmed by fluorescence microscopy. Finally, in vitro binding study on BT4C cells expressing lodavin fusion protein, bearing the extracellular avidin moiety, showed that such biotin-covered PLNP could successfully be targeted to malignant glioma cells through a specific biotin-avidin interaction. The influence of nanoparticle core diameter, incubation time, and PLNP concentration on the efficiency of targeting is discussed.

Dual mode NIR long persistent phosphorescence and NIR-to-NIR Stokes luminescence in La3Ga5GeO14: Cr3+, Nd3+ phosphor

“…Researchers want to selectively excite only part of the experimental object and/or to re-excite the injected luminescent material after its luminescence vanishes in particular cases [21][22][23]. Due to the inherent intensity attenuation over time of LLPs, the valuable detection time is too short to guarantee the vigorous repeated imaging at long time intervals and photo-realistic local imaging, which limits the use of the persistent luminescent material for bio-imaging applications [24].…”

Chemically engineered persistent luminescence nanoprobes for bioimaging

“…Biodistribution in healthy mice was achieved after systemic injection to validate the use of this probe for in vivo applications 42, 43, 44. For that purpose, BALB/c mice were injected with a 100 µL colloidal suspension of 1 mg of PLNPs suspended in 1 mL of sterile 150 mM NaCl.…”

Photoluminescent nanoplatforms in biomedical applications

“…Their ability to undergo optical excitation before being incubated with the targeted cells provides high improvement to the signal-to-noise ratio. The in vitro visualization of glioma cells [111], RAW cells [112], HeLa cells [113], and prostate cancer cells [114] has been achieved.…”