In vitro systems in the study of peroxisomal protein import

Baker, Alison

doi:10.1007/bf01952102

Cited by 6 publications

(2 citation statements)

References 48 publications

(50 reference statements)

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“…Most peroxisomal enzymes are synthesized on free polysomes in the cytosol and then imported into peroxisomes (Baker 1996;Olsen and Harada 1995;Subramani 1993). In plants, yeasts and animals, peroxisomal enzymes have a peroxisomal targeting signal at the C-terminus (PTS1) or N-terminus (PTS2).…”

Section: Introductionmentioning

confidence: 99%

Visualization of microbodies in Chlamydomonas reinhardtii

Hayashi

Shinozaki

2011

J Plant Res

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In Chlorophycean algal cells, these organelles are generally called microbodies because they lack the enzymes found in the peroxisomes of higher plants. Microbodies in some algae contain fewer enzymes than the peroxisomes of higher plants, and some unicellular green algae in Chlorophyceae such as Chlamydomonas reinhardtii do not possess catalase, an enzyme commonly found in peroxisomes. Thus, whether microbodies in Chlorophycean algae are similar to the peroxisomes of higher plants, and whether they use a similar transport mechanism for the peroxisomal targeting signal (PTS), remain unclear. To determine whether the PTS is present in the microbodies of Chlorophycean algae, and to visualize the microbodies in Chlamydomonas cells, we examined the sub-cellular localization of green fluorescent proteins (GFP) fused to several PTS-like sequences. We detected GFP compartments that were spherical with a diameter of 0.3-1.0 μm in transgenic Chlamydomonas. Comparative analysis of the character of GFP-compartments observed by fluorescence microscopy and that of microbodies by electron microscopy indicated that the compartments were one and the same. The result also showed that the microbodies in Chlorophycean cells have a similar transport mechanism to that of peroxisomes of higher plants.

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Section: Introductionmentioning

confidence: 99%

Visualization of microbodies in Chlamydomonas reinhardtii

Hayashi

Shinozaki

2011

J Plant Res

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“…In vitro import assays have apparently been established for plant peroxisomes (Baker 1996;Brickner et al 1997;Pool et al 1998;reviewed by Baker et al 2000), which will allow further studies on the tasks of single components of the import machinery. A limiting factor was the low number of plant peroxins which had not been identified until recently (Baker et al 2000;Charlton and Lopez-Huertas 2002; Table 1).…”

Section: Morphology and Metabolismmentioning

confidence: 99%

Peroxisome biogenesis

Eckert

Erdmann

Reviews of Physiology, Biochemistry and Pharmacology

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Peroxisome biogenesis conceptually consists of the (a) formation of the peroxisomal membrane, (b) import of proteins into the peroxisomal matrix and (c) proliferation of the organelles. Combined genetic and biochemical approaches led to the identification of 25 PEX genes-encoding proteins required for the biogenesis of peroxisomes, so-called peroxins. Peroxisomal matrix and membrane proteins are synthesized on free ribosomes in the cytosol and posttranslationally imported into the organelle in an unknown fashion. The protein import into the peroxisomal matrix and the targeting and insertion of peroxisomal membrane proteins is performed by distinct machineries. At least three peroxins have been shown to be involved in the topogenesis of peroxisomal membrane proteins. Elaborate peroxin complexes form the machinery which in a concerted action of the components transports folded, even oligomeric matrix proteins across the peroxisomal membrane. The past decade has significantly improved our knowledge of the involvement of certain peroxins in the distinct steps of the import process, like cargo recognition, docking of cargo-receptor complexes to the peroxisomal membrane, translocation, and receptor recycling. This review summarizes our knowledge of the functional role the known peroxins play in the biogenesis and maintenance of peroxisomes. Ideas on the involvement of preperoxisomal structures in the biogenesis of the peroxisomal membrane are highlighted and special attention is paid to the concept of cargo protein aggregation as a presupposition for peroxisomal matrix protein import.

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Targeting and Import of Matrix Proteins into Peroxisomes

Mullen

2002

Plant Peroxisomes

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In vitro systems in the study of peroxisomal protein import

Cited by 6 publications

References 48 publications

Visualization of microbodies in Chlamydomonas reinhardtii

Visualization of microbodies in Chlamydomonas reinhardtii

Peroxisome biogenesis

Targeting and Import of Matrix Proteins into Peroxisomes

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