In vitro synthesis of oviductal secretory proteins by estrogen‐treated ovariectomized gilts

Abstract

The objective of this study was to identify, characterize, and examine oviductal secretory proteins (OSP) synthesized de novo by whole oviduct (WO), ampulla (A), and isthmic (I) tissue from ovariectomized (OVX), corn oil (CO)-, estrogen (E)-, progesterone (P)-, and E + P-treated gilts. Oviducts were collected from OVX gilts after CO, E, P, or E + P treatment for 11 consecutive days and tissue was incubated with 3H-leucine (3H-leu). Rates of 3H-leu incorporation into nondialyzable macromolecules by WO explants were greater (P less than 0.01) with E- compared to CO-, P-, or E + P-treated gilts and greater (P less than 0.05) by A explants with E- compared to CO-, P-, or E + P-treated gilts. An effect of location was noted, with A having a greater (P less than 0.01) rate of incorporation than WO or I. Conditioned culture medium was analyzed by one (1D)- and two-dimensional (2D) sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis (PAGE) and fluorography. Analyses by 1D-SDS-PAGE revealed three major E-dependent bands (335,000, 100,000, and 80,000 M(r)) in WO and A, and one (335,000 M(r)) in the I. A 20,000 M(r) band found in A was inhibited by E, while a 60,000 M(r) band found in the A was induced by P. Analyses by 2D-SDS-PAGE resolved major E-dependent bands 2 (100,000 M(r)) and 3 (80,000 M(r)) into basic and acidic 100,000 M(r) proteins and a 75,000-85,000 M(r) protein (pI less than 4), respectively, found in WO and A, but not in I. A basic 20,000 M(r) protein and an acidic 45,000 M(r) complex, both found in A, were inhibited by E. Gel filtration of culture medium revealed a high M(r) fraction (greater than 2 x 10(6)) that was induced by E and was 6.8-fold greater in medium from A than from I. This study clearly demonstrates that 1) WO and A tissue from E-treated gilts de novo synthesize and secrete three major proteins (basic 100,000, acidic 100,000, and 75,000-85,000 M(r)); 2) these E-dependent proteins are not found in I or with other treatment; 3) several protein complexes synthesized by A are inhibited by E treatment; and 4) a high M(r) fraction, produced primarily in the A, is induced or amplified by E.

Distribution of sialoglycoconjugates in the oviductal isthmus of the horse during anoestrus, oestrus and pregnancy: a lectin histochemistry study

“…Glycoproteins secreted by non-ciliated cells create an intraluminal environment able to immobilise spermatozoa (Hunter, 1995), to maintain the viability and fertilizing capability of spermatozoa (Pollard et al, 1991;Suarez et al, 1991;Chian et al, 1995) and to play a supportive role in sperm/egg interactions (Geng et al, 1997;Tulsiani et al, 1997). Differences in the production of oestrus-associated glycoproteins by oestrogen have been observed in the isthmus of baboon (Verhage and Fazleabas, 1988) and pig (Buhi et al, 1992).…”

“…In particular, the synthesis and secretion of oviduct-specific protein have been shown to be controlled by ovarian steroids (Erickson-Lawrence et al, 1989;Buhi et al, 1992;DeSouza and Murray, 1995).…”

Oviductins possess chitinase‐ and mucin‐like domains: A lead in the search for the biological function of these oviduct‐specific ZP‐associating glycoproteins

“…Estrogen treatment of ovariectomized animals can also restore oviductin production (Oliphant et al, 1984a;Buhi et al, ,1992Verhage et al, 1989Verhage et al, , 1990. The cyclical variations of oviductin synthesis and secretion in other mammals are more subtle, possibly because of the shorter duration of their ovarian cycles.…”

“…Indeed, while it has been clearly demonstrated that some of these oviduct-specific secretory proteins are released in response to endogenous estrogen at estrus in the mouse (Horvat et al, 1992), sheep (Sutton et al, 1986;Murray, 19931, human (Verhage et al, 1988), cow (Malayer et al, 1988;Gerena and Killian, 1990; Abe et al, 1993b), baboon (Ver- , and gilt (Buhi et al, 1989(Buhi et al, , 1990 or in ovariectomized estrogen-treated animals (Oliphant et al, 1984b;Buhi et al, ,1992Murray, 1993;) the role of steroid sex hormones in the control of the secretion (Malette et al, 1994) or mRNA expression (Donnelly et al, 1991;Arias et al, 1994) of others seems to be different. Some of these oviduct-specific secretory glycoproteins possess the interesting properties of interaction with the zona pellucida (zp) and/or perivitelline (PV) space of the oocyte during its passage through the oviduct and remain associated with the embryo until implantation.…”

Influence of Estrogen and Progesterone on Ultrastructural Indices of Oviductal Epithelium in Sexually Immature Mice

“…Mutual interactions between gametes and oviductal epithelium were studied during their co-cultivation in the medium saturated with steroid hormones (Reuter et al 1994). An increased secretion of the oviductal epithelium was observed after the administration of both estrogen and progesterone (Erickson-Lawrence et al 1989;Buhi et al 1991;Buhi et al 1992). Sensitivity of the structural components of the oviduct to ovarian steroid hormones was confirmed by several authors, such as Nozaki and Ito (1987), Elsimar and Coutinho (1988), Faussone-Pelegrini and Bani (1990), Hoshino and Kumasaka (1991).…”