In Vitro Synthesis and Membrane Integration of the Chloroplast Encoded D-2 Protein of Photosystem II

Friemann, Andreas; Schwarz, H. J.; Hachtel, Wolfgang

doi:10.1007/978-1-4615-3366-5_39

Regulation of Chloroplast Biogenesis 1992

DOI: 10.1007/978-1-4615-3366-5_39

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In Vitro Synthesis and Membrane Integration of the Chloroplast Encoded D-2 Protein of Photosystem II

Andreas Friemann

H. J. Schwarz

Wolfgang Hachtel

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1992

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“…This system has been used to determine whether the precursor or the mature protein is integrated into the thylakoid membrane and to study the assembly with chlorophylls and carotenoids during the integration process [19]. Similarly, assembly of the photosystem-I (PS I) [20] or the PS I1 [21] reaction center complexes in the thylakoid membrane have been studied. Since ELIP species are distant relatives of light-harvesting complex (LHC) proteins, this system will allow us to analyze ELIP topology within natural and artificial membranes, its possible association with pigments, the interaction with neighbouring proteins and the effect of in-vitro mutagenesis on these various aspects.…”

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Integration of early light‐inducible proteins into isolated thylakoid membranes

Kruse

Kloppstech

1992

European Journal of Biochemistry

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An in-vitro system has been established to study the integration of early light-inducible proteins (ELIP) into isolated thylakoid membranes. The in-vitro-expressed ELIP precursor proteins exist in two forms, a high-molecular-mass aggregate which is accessible to trypsin but no longer to the stromal processing protease and a soluble form which is readily cleaved to the mature form by the stromal protease. The mature form of ELIP is integrated into thylakoid membranes; its correct integration can be deduced from the observation that the posttranslationally transported products and the invitro integrated ELIP species are cleaved by trypsin to products of the same apparent molecular mass. Trypsin-resistant fragments of high-molecular-mass and low-molecular-mass ELIP appear to have the same size. The processed ELIP species, as well as an engineered mature form of ELIP, are integrated into isolated thylakoid membranes. Integration of the mature protein occurs in the absence of stroma, into sodium-chloride-washed, and trypsin-treated thylakoid membranes. The process of integration is almost temperature independent over 0 -30 "C. Analysis of the time course ofintegration leads to the conclusion that, under in-vitro conditions, processing but not integration into membranes is the rate-limiting step. In the absence of stroma, the ELIP precursor is bound to the thylakoid membranes, however, it is no longer accessible to the stromal maturating protease when added after binding has occurred. In conclusion, integration of ELIP differs in many essential details from that of its relatives, the light-harvesting chlorophyll u/b protein family.Early light-inducible proteins (ELIP) are short lived, thylakoid-membrane-located proteins whose genes are transiently transcribed after illumination of etiolated pea [l] or barley plantlets [2, 31. Whilc, to date, only one protein has been found in pea [l, 41, two small multigene families of different size have been characterized in barley [2].The expression of ELIP genes is not restricted to etiolated plantlets as their genes are transcribed in green plants early in the morning and under the control of the circadian clock [5]. Both in vivo [6] and after in-vitro import into intact chloroplasts [I, 71, ELIP species are integrated into the thylakoid membranes. By means of detergent fractionation of cross-linked thylakoid membranes, we found that ELIP species are associated with a preparation of photosystem-I1 (PS 11) particles [7, 81 and, in particular, with the D1 protein.D1, as a constituent of PS 11, has been found prevalently in the grana thylakoid membrane fraction [9], while a considerable portion of ELIP is obtained from the stroma thylakoid membranes [6, 71 and the intermediate membrane preparations [7]. These findings indicate that the association of EIJP with PS I1 should be confined to the site of insertion of preDl into the PS I1 particles [lo].The turnover and, consequently, the integration of D1 is accelerated after damage of PS I1 reaction centers due to highlight stress, a...

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confidence: 99%

Integration of early light‐inducible proteins into isolated thylakoid membranes

Kruse

Kloppstech

1992

European Journal of Biochemistry

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In Vitro Synthesis and Membrane Integration of the Chloroplast Encoded D-2 Protein of Photosystem II

Cited by 1 publication

References 14 publications

Integration of early light‐inducible proteins into isolated thylakoid membranes

Integration of early light‐inducible proteins into isolated thylakoid membranes

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