In vitro synergy and postantibiotic effect of colistin combinations with meropenem and vancomycin against Enterobacteriaceae with multiple carbapenem resistance mechanisms

Bedenić, Branka; Car, Haris; Slaćanac, Domagoj; Sviben, Mario; Čačić, Marko; Lukić-Grlić, Amarela; Benčić, Ana; Šijak, Dorotea; Tripković, Marko; Beader, Nataša

doi:10.1016/j.jiac.2018.06.001

Cited by 7 publications

(5 citation statements)

References 12 publications

(11 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this article, we developed an intelligent drug delivery microcarrier with a pathological state responsive switch, by combining an inverse opal scaffold generated by a temperature-responsive hydrogel and biodegradable sodium alginate hydrogel used to encapsulate drugs. Inverse opal particles possess unparalleled features of uniform, three-dimensional interconnecting pores and specific optical characteristics, which makes them ideal scaffolds in drug delivery and tissue engineering. − In addition, vancomycin, the most important treatment for methicillin-resistant Staphylococcus aureus (MRSA) infections, , can be loaded into the inverse opal pores and encapsulated by a second biocompatible gel. Therefore, the combination of inverse opal scaffolds and vancomycin could be conducive to the development of microcarriers for promoting wound healing.…”

Section: Introductionmentioning

confidence: 99%

Antibacterial Porous Microcarriers with a Pathological State Responsive Switch for Wound Healing

Chen

Liu

Sun

et al. 2019

ACS Appl. Bio Mater.

View full text Add to dashboard Cite

In this article, novel antibacterial porous microcarriers with a pathological state responsive switch were developed to promote wound healing. The porous microcarrier includes an inverse opal scaffold generated by a temperatureresponsive hydrogel that deforms according to different temperatures and a biodegradable sodium alginate hydrogel used as a vehicle to load drugs. Thus, the microcarrier is endowed with the ability of releasing loaded drugs when the temperature changes in the wound site due to an inflammation reaction. Without inflammation, the microcarriers will be locked and the release of drug molecules will not continue, which prevents the abuse of drugs and further improves the safety of the therapeutic treatment. The designed microcarriers could eliminate inflammation significantly and promote the formation of granulation tissue, angiogenesis, and collagen deposition in the wound site. These features indicate that these microcarriers with a pathological state responsive switch are ideal for promoting wound healing and will have a great potential in biomedical applications.

show abstract

Section: Introductionmentioning

confidence: 99%

Antibacterial Porous Microcarriers with a Pathological State Responsive Switch for Wound Healing

Chen

Liu

Sun

et al. 2019

ACS Appl. Bio Mater.

View full text Add to dashboard Cite

show abstract

“…9 However, another study reported that intravenous colistin combination therapy versus colistin monotherapy against MDR Gram negative bacteria was not associated with lower mortality rate among patients. 10 Other studies conducted on carbapenem-resistant K. pneumoniae revealed that colistin and meropenem combination showed synergistic effect by checkerboard, 12 and ertapenem containing double carbapenem was associated with microbiological success observed among 79% of patients 24 and that double carbapenem therapy for patients with bacteremia was associated with 67% clinical success and with 100% microbiological eradication. 25…”

Section: Discussionmentioning

confidence: 99%

“…10,11 Additionally, the clinical studies had reported on improved clinical outcomes with combination therapy even if the tested isolates were resistant to individual drugs. 12…”

Section: Introductionmentioning

confidence: 99%

XDR-Klebsiella pneumoniae isolates harboring bla_OXA-48: In vitro and in vivo evaluation using a murine thigh-infection model

Kamel

Eltayeb

El-Ansary

et al. 2019

Exp Biol Med (Maywood)

View full text Add to dashboard Cite

Blood stream infection with extensively drug-resistant-carbapenamase producing Klebsiella (K.) pneumoniae usually represents a major threat with medical challenges among hospitalized cancer patients with poor functional status and underlying diseases. Accordingly, the aim of the study was to evaluate the efficacy of different antibiotics either alone or in combinations against extensively drug-resistant-OXA-48 producing K. pneumoniae clinical isolates that were previously recovered from febrile neutropenic pediatric cancer patients. The antimicrobial activity of amikacin, gentamicin, colistin, ertapenem, imipenem, meropenem and tigecycline was assessed by broth microdilution method. The results revealed that all the tested OXA-48 producing K. pneumoniae isolates exhibited extensively drug-resistant phenotype and all of them were susceptible to tigecycline. Checkerboard method was used to determine the fraction inhibitory concentration index, to further classify the effect of antibiotic combination as synergistic, additive, indifferent, or antagonistic effect. The results revealed that in vitro dual carbapenem combination of ertapenem with meropenem had shown synergistic effect against all of the tested isolates. Additionally, synergistic effect of meropenem with colistin was detected among three of four isolates tested. Herein we investigated the in vivo activity of colistin, meropenem alone and in combination in a rat thigh infection model. The results showed that addition of meropenem to colistin was not effective at reduction of bacterial count as compared to colistin alone at 24 h post treatment. Accordingly, we can conclude that in vitro antibiotic combinations of dual carbapenems (ertapenem plus meropenem) and meropenem plus colistin showed synergism in 100% and 75% of the tested isolates, respectively. Colistin alone had significantly reduced bacterial count while its combination with meropenem was not superior to monotherapy in murine thigh infection model. Impact statement The present study aimed to evaluate the effectiveness of various antibiotics both in vitro and in vivo using murine animal model either alone or in combination against various strains of extensively drug-resistant (XDR) Klebsiella pneumoniae, life-threatening pathogens of relevant medical importance isolated from febrile neutropenic pediatric cancer patients. This work also emphasizes how to select the appropriate antibiotics options and help the physicians to choice the appropriate antibiotic for the treatment of such superbugs (extensively drug-resistant (XDR) Klebsiella pneumoniae). The results showed that in vitro dual carbapenem combination of ertapenem with meropenem had shown synergistic effect against all of the tested XDR isolates. Antibiotic combinations of dual carbapenems and meropenem plus colistin showed synergism in 100% and 75% of the testes isolates, respectively. Results of the in vivo evaluation, colistin alone had significantly reduced bacterial count while its combination with meropenem was not superior to monotherapy.

show abstract

“…A kinetic time-kill experiment was performed based on a modified method of Segovia et al [19, 20]. Two standard S. aureus strains were separately grown in MH broth medium with a final count of approximately 1 × 10 5 CFU/mL.…”

Section: Methodsmentioning

confidence: 99%

Characterization of the anti-Staphylococcus aureus fraction from Penthorum chinense Pursh stems

Ding

Zhang

et al. 2019

BMC Complement Altern Med

View full text Add to dashboard Cite

Background Methicillin-resistant Staphylococcus aureus (MRSA) causes serious infections in hospitals. Penthorum chinense Pursh (PCP), employed by the Miao ethnic minority in China, presents antibacterial activities. In this study, the anti- Staphylococcus aureus activities in the pinocembrin-7-O residue-rich fraction from PCP (PGF) were evaluated and characterized. Methods The PGF was prepared with 70% ethanol reflux extraction followed by fractional extraction and column chromatography. Pinocembrin-7-O residue components were identified with electrospray ionization mass spectrometry (ESI-MS). Anti- S. aureus activities of the fraction and the main components were evaluated in vitro with serially diluted microbroth assays. Cytotoxicity was evaluated with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) chromogenic assays using the NCTC 1469 cell line. Results This study indicated that the PGF and three components (S1, S2, and S3) presented anti- S. aureus activities, including against clinically isolated MRSA strains. The molecular masses of S1, S2, and S3 were identical to those of pinocembrin-7-O-[4″,6″-hexahydroxydiphenoyl (HHDP)]-β-D-glucose, pinocembrin-7-O-[3″-O-galloyl-4″,6″-(s)-HHDP]-β-D-glucose, and Thonningianin A, respectively. The PGF, S1, S2, and S3 all presented an identical minimum inhibitory concentration (MIC) against S. aureus ATCC 25923 and ATCC 43300, which was 62.5 μg/mL. The minimum bactericidal concentrations (MBCs) of the PGF and S3 against ATCC 25923 were 125 and 250 μg/mL, and the MBCs of the PGF, S2, and S3 against ATCC 43300 were 250, 500, and 250 μg/mL, respectively. A time-kill assay consistently indicated that none of the bacterial clones of ATCC 25923 and ATCC 43300 could survive under 2× and 4× MIC PGF treatment for 24 h, respectively. In contrast, 10 4 CFU (colony-forming units) of ATCC 25923 and ATCC 43300 were killed by 8× and 4× MIC S3 within 24 h, respectively. Additionally, 1×, 2×, and 4× MIC the PGF presented similar postantibiotic effects (PAEs) on the strain ATCC 25923. However, the PAE of the PGF on the strain ATCC 43300 was concentration dependent (1× < 2× < 4× MIC). Finally, the PGF (200 μg/mL) and S3 (60 μg/mL) showed no cytotoxicity against human hepatoma cells. Conclusions The PGF and S3 from PCP present potential for the treatment of S. aureus and MRSA infections. The components S1 and S2 present inhibition activities against S. aureus .

show abstract

In vitro synergy and postantibiotic effect of colistin combinations with meropenem and vancomycin against Enterobacteriaceae with multiple carbapenem resistance mechanisms

Cited by 7 publications

References 12 publications

Antibacterial Porous Microcarriers with a Pathological State Responsive Switch for Wound Healing

Antibacterial Porous Microcarriers with a Pathological State Responsive Switch for Wound Healing

XDR-Klebsiella pneumoniae isolates harboring bla_OXA-48: In vitro and in vivo evaluation using a murine thigh-infection model

Characterization of the anti-Staphylococcus aureus fraction from Penthorum chinense Pursh stems

Contact Info

Product

Resources

About

In vitro synergy and postantibiotic effect of colistin combinations with meropenem and vancomycin against Enterobacteriaceae with multiple carbapenem resistance mechanisms

Cited by 7 publications

References 12 publications

Antibacterial Porous Microcarriers with a Pathological State Responsive Switch for Wound Healing

Antibacterial Porous Microcarriers with a Pathological State Responsive Switch for Wound Healing

XDR-Klebsiella pneumoniae isolates harboring blaOXA-48: In vitro and in vivo evaluation using a murine thigh-infection model

Characterization of the anti-Staphylococcus aureus fraction from Penthorum chinense Pursh stems

Contact Info

Product

Resources

About

XDR-Klebsiella pneumoniae isolates harboring bla_OXA-48: In vitro and in vivo evaluation using a murine thigh-infection model