In Vitro Susceptibility of Mycoplasma hyosynoviae and M. hyorhinis to Antimicrobial Agents.

Kobayashi, Hideki; Sonmez, Nejdet; Morozumi, Tatsuya; Mitani, Kenji; Ito, Nobuyoshi; Shiono, Hiroki; Yamamoto, Koshi

doi:10.1292/jvms.58.11_1107

Cited by 26 publications

(16 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Sixteen of twenty (80%) M. hyorhinis isolates were susceptible to tylosin, and most of the tylosin-resistant isolates were from Japan, which is in agreement with recent investigations (16). Resistance to tylosin for Japanese M. hyosynoviae isolates (17) was observed in one of five isolates. Isolation of Mycoplasma species from tissues requires successive subculturing, and it has been described that during this process resistance may disappear (16,17).…”

supporting

confidence: 90%

“…Resistance to tylosin for Japanese M. hyosynoviae isolates (17) was observed in one of five isolates. Isolation of Mycoplasma species from tissues requires successive subculturing, and it has been described that during this process resistance may disappear (16,17). It can be speculated that the resistance to macrolides in vivo can be higher than described here.…”

mentioning

confidence: 98%

See 1 more Smart Citation

Comparative susceptibilities of various animal-pathogenic mycoplasmas to fluoroquinolones

Hannan¹,

Windsor²,

Jong³

et al. 1997

Antimicrob Agents Chemother

137

View full text Add to dashboard Cite

The in vitro activities of six antimicrobial agents were tested against 162 mycoplasma strains of eight species isolated from poultry and livestock at different geographic sites. Tiamulin was most active (MICs at which 90% of the isolates were inhibited [MIC90s], 0.025 to 0.25 microg/ml); enrofloxacin and danofloxacin had near equivalent activities (MIC90s, 0.05 to 1.0 microg/ml), but were much more active than flumequine (MIC90s, 1 to 50 microg/ml). The MIC90s of tylosin and oxytetracycline were 0.25 to > 100 microg/ml and 0.25 to 100 microg/ml, respectively.

show abstract

supporting

confidence: 90%

mentioning

confidence: 98%

Comparative susceptibilities of various animal-pathogenic mycoplasmas to fluoroquinolones

Hannan¹,

Windsor²,

Jong³

et al. 1997

Antimicrob Agents Chemother

137

View full text Add to dashboard Cite

show abstract

“…The minimal inhibitory concentration of each antimicrobial agent was determined by applying an agar dilution method with some modifications and in accordance with the recommendations of Hannan (2000) and Kobayashi et al (1996). Seven antimicrobial agents were included in the testing: tulathromycin (Pfizer, United States), tylosin (Chemifarma, Italia), lincomycin (Pfizer, United States), enrofloxacin (Vetoquinol, Austria), florfenicol (Krka, Slovenia), oxytetracycline (Chemifarma, Italia),) and penicillin G (Actavis Baltics, Lithuania).…”

Section: Antimicrobial Agentsmentioning

confidence: 99%

Susceptibility of Mycoplasma bovis field isolates to antimicrobial agents

Šiugždaitė¹,

Gabinaitienė²,

Kerzienė³

2012

Vet. Med. - Czech

View full text Add to dashboard Cite

ABSTRACT:The purpose of this study was to determine the antibacterial susceptibility of field isolates of Mycoplasma bovis originating from the upper respiratory tract of cattle of different ages. Bacteriological examination of 90 nasal swabs collected from calves at three months of age identified M. bovis in 31 (34.44%) samples. Seventeen (18.88%) of these animals still housed M. bovis in their nasal cavity at nine months and five animals (5.55%) still at seventeen months of age. M. bovis were confirmed by biochemical and antigenic methods. To confirm that these belonged to the M. bovis species isolated mycoplasmas were tested using the PCR method. Fifteen field strains of M. bovis isolated from the same cattle at three, nine and seventeen months (five strains from each age group) were selected for antibacterial susceptibility testing against six groups of antimicrobial agents using an agar dilution method. The MIC 90 ranges established for tylosin, tulathromycin, enrofloxacin, florfenicol and lincomycin were 0.39-0.78 µg/ml, 0.50-1.00 µg/ml, 0.78-1.56 µg/ml, 3.12 µg/ml and 0.39-0.78 µg/ml, respectively. The range of MIC 90 for oxytetracycline was from 50 to 100 µg/ml. Preliminary examination of the antimicrobial susceptibility of field strains of M. bovis did not reveal significant differences between different age groups of cattle. After evaluation of the MIC 90 data with the SPSS 13.0 statistical package it was found that M. bovis isolates from animals at three, nine and seventeen months were similarly susceptible to tylosin and tulathromycin. Statistically significant differences in susceptibility of M. bovis isolated from cattle of different ages were found to florfenicol compared with tulathromycin (P < 0.01), lincomycin (P < 0.01) and enrofloxacin (P < 0.05). The susceptibility of all M. bovis isolates to oxytetracycline and penicillin G significantly differed from the sensitivity to all other antimicrobial agents used in the present study (P < 0.05). The in vitro susceptibility test showed that field isolates of M. bovis isolated from cattle of different ages were similarly sensitive to tylosin, tulathromycin, lincomycin and enrofloxacin. It was also determined that the field strains are resistant to oxytetracycline.

show abstract

“…We also compared the activities of these peptides with that of enrofloxacin, a fluoroquinolone that is used in veterinary practice and that is known to have potent activity against mycoplasmas (23). In addition, we have attempted to develop a method, based upon the use of these peptides, for the eradication of mycoplasmas from human tissue cell cultures.…”

mentioning

confidence: 99%

Susceptibilities of Mycoplasma fermentans and Mycoplasma hyorhinis to Membrane-Active Peptides and Enrofloxacin in Human Tissue Cell Cultures

Nir-Paz

Prévost

Nicolas

et al. 2002

Antimicrob Agents Chemother

View full text Add to dashboard Cite

Mycoplasmas, which are bacteria that are devoid of a cell wall and which belong to the class Mollicutes, are pathogenic for humans and animals and are frequent contaminants of tissue cell cultures. Although contamination of cultures with mycoplasma can easily be monitored with fluorescent dyes that stain DNA and/or with molecular probes, protection and decontamination of cultures remain serious challenges. In the present work, we investigated the susceptibilities of Mycoplasma fermentans and Mycoplasma hyorhinis to the membrane-active peptides alamethicin, dermaseptin B2, gramicidin S, and surfactin by growth inhibition and lethality assays. In the absence of serum, the four peptides killed mycoplasmas at minimal bactericidal concentrations that ranged from 12.5 to 100 M, but in all cases the activities were decreased by the presence of serum. As a result, under standard culture conditions (10% serum) only alamethicin and gramicidin S were able to inhibit mycoplasma growth (MICs, 50 M), while dermaseptin B2 and surfactin were ineffective. Furthermore, 8 days of treatment of HeLa cell cultures experimentally contaminated with either mycoplasma species with 70 M enrofloxacin cured the cultures of infection, whereas treatment with alamethicin and gramicidin S alone was not reliable because the concentrations and treatment times required were toxic to the cells. However, combination of alamethicin or gramicidin S with 70 M enrofloxacin allowed mycoplasma eradication after 30 min or 24 h of treatment, depending on the mycoplasma and peptide considered. HeLa cell cultures experimentally infected with mycoplasmas should prove to be a useful model for study of the antimycoplasma activities of antibiotics and membrane-active peptides under conditions close to those found in vivo.

show abstract

In Vitro Susceptibility of Mycoplasma hyosynoviae and M. hyorhinis to Antimicrobial Agents.

Cited by 26 publications

References 16 publications

Comparative susceptibilities of various animal-pathogenic mycoplasmas to fluoroquinolones

Comparative susceptibilities of various animal-pathogenic mycoplasmas to fluoroquinolones

Susceptibility of Mycoplasma bovis field isolates to antimicrobial agents

Susceptibilities of Mycoplasma fermentans and Mycoplasma hyorhinis to Membrane-Active Peptides and Enrofloxacin in Human Tissue Cell Cultures

Contact Info

Product

Resources

About