In vitro susceptibility of Candida   albicans clinical isolates to eight antifungal agents in Ouagadougou (Burkina Faso)

Zida, Adama; Yacouba, Abdourahamane; Bamba, Sanata; Sangaré, Ibrahim; Sawadogo, Mamadou; Guiguemdé, T. R.; Kone, S.; Traoré, L.; Ouédraogo-Traoré, Rasmata; Guiguemdé, Robert Tinga

doi:10.1016/j.mycmed.2017.07.001

Cited by 25 publications

(15 citation statements)

References 23 publications

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“…In the present study, all of the Candida strains of cancer patients exhibited low MIC values to AMB with a GM of 0.77 μg/ml which is inconsistent with the results of previous studies that reported high resistance to AMB [ 37 , 34 ]. However, it is similar to the findings of some of the previous studies that indicated most isolates of C. albicans were sensitive to AMB [ 38 , 34 ].…”

Section: Discussioncontrasting

confidence: 99%

Molecular identification and antifungal susceptibility testing of Candida species isolated from oral lesions in patients with head and neck cancer undergoing radiotherapy

Kermani¹,

Sadeghian²,

Shokohi³

et al. 2021

CMM

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Background and Purpose: Radiation therapy in patients with head and neck malignancies predisposes them to oral Candida colonization and infection due to damage of oral mucosa and destruction of the salivary gland. This study aimed to determine the prevalence of oropharyngeal candidiasis (OPC) in patients with head and neck cancer (HNC) undergoing radiotherapy (RT), identify the yeasts isolated from them, and determine their antifungal susceptibility. Materials and Methods: This cross-sectional study was conducted from December 2018 to June 2019 at two referral radiotherapy centers in northern Iran. Yeast strains that were isolated from patients with HNC were identified using conventional and molecular methods. The in vitro activities of eight common antifungal drugs against 55 isolates were investigated according to the guidelines of the Clinical and Laboratory Standard Institute (M27-A3 and M27-S4) broth microdilution document. Results: Among 59 patients receiving RT, the prevalence of OPC was 21 (35.59%) and 15 (25.42%) patients were diagnosed with colonization. The mean age of the patients was 55.32±13.3 years (within the range of 27-87 years). In this study, the pseudomembranous form was reported as the most clinical type of OPC. Candida albicans with the frequency of 60% was the most common type of Candida spp. that was observed in this study, although non-albicans Candida spp., such as C. glabrata (27.27%), C. tropicalis (5.45%), C. parapsilosis (3.63%), C. krusei (1.83%), and C. kefyr(1.83%) were also isolated. Considering the low minimum inhibitory concentration range of amphotericin B, compared to fluconazole, administration of this agent is a more suitable antifungal drug for extensive oral candidiasis in these patients. Among azoles,clotrimazole had low efficacy and several studied isolates (65.5%) showed resistance. Conclusion: Correct diagnosis as well as determining drug sensitivity and risk factors are the effective steps in reducing the complications related to oral candidiasis in people undergoing RT.

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Section: Discussioncontrasting

confidence: 99%

Molecular identification and antifungal susceptibility testing of Candida species isolated from oral lesions in patients with head and neck cancer undergoing radiotherapy

Kermani¹,

Sadeghian²,

Shokohi³

et al. 2021

CMM

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“…In our study, out of 262 Candida clinical isolates, C. albicans remains to be the most common species at 59.90% followed by Candida glabrata 27.86%. This results are similited to Zida et al study who obtained a prevalence of 59.36% of C. albicans [ 8 ] and also Kouadio-Yapo et al, a high prevalence of 64.8% in Ivory Coast [ 16 ].…”

Section: Discussionsupporting

confidence: 87%

First detection of mutated ERG11 gene in vulvovaginal Candida albicans isolates at Ouagadougou/Burkina Faso

et al. 2022

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Background Vulvovaginal candidiasis is an important cause of morbidity among women due to Candida species. In the last decades, resistance to azoles, first-line antifungals has increased. One molecular mechanism of azole resistance by Candida involves mutations in the ERG11 gene encoding lanosterol 14-α-demethylase, the target enzyme. This study was conducted to identify the clinical Candida species associated in vulvovaginal candidiasis; to determine the rate of antifungal resistance among Candida albicans isolates and to determine mutated ERG11 gene at Saint Camille Hospital in Ouagadougou, Burkina Faso. Methods Antifungals susceptibility were performed using Kirby–Bauer disk diffusion method. ERG11 gene was detected using conventional PCR in C. albicans isolates resistant to at least one azole. Results Out of 262 clinical strains isolated, C. albicans accounted for 59.90%, followed by Candida glabrata 27.86%, Candida famata 7.25%, Candida tropicalis 3.05% and Saccharomyces cerevisiae 1.91%. Resistance rate of fluconazole to C. albicans was 59.54%. ERG11 gene was found in 9.79% of 92 C. albicans strains resistant to azoles. Conclusions This detection of mutated ERG11 gene in C. albicans is the first in Burkina Faso and may be a cause of azole resistance in recurrent Candida vulvovaginitis.

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“…On the other hands, a lower resistance rate was reported in Egypt 11% 39 . Regarding Itraconazole resistance, as few as 4 isolates of C. krusie and C. glabrata were resistant, this is in contrast to the finding from Ethiopia and Burkina Faso in which itraconazole resistance was as higher as 50 and 52% respectively 37 , 40 . This variation in susceptibility rate might be attributed to difference in previous exposure to fluconazole since several studies have described that azole resistance is highly associated with previous exposure to fluconazole 40 , 41 .…”

Section: Discussioncontrasting

confidence: 60%

“…Regarding Itraconazole resistance, as few as 4 isolates of C. krusie and C. glabrata were resistant, this is in contrast to the finding from Ethiopia and Burkina Faso in which itraconazole resistance was as higher as 50 and 52% respectively 37 , 40 . This variation in susceptibility rate might be attributed to difference in previous exposure to fluconazole since several studies have described that azole resistance is highly associated with previous exposure to fluconazole 40 , 41 . Concerning azole cross-resistance, all isolates that were itraconazole resistant were fluconazole resistance, similar finding was obtained previously in which the cross-resistance rate was 83%.…”

Section: Discussioncontrasting

confidence: 60%

Detection of antifungal drug-resistant and ERG11 gene mutations among clinical isolates of Candida species isolated from Khartoum, Sudan.

Mohamed

Hussain

et al. 2021

F1000Res

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Background: Candida species are one of the most important opportunistic fungal pathogens that cause both superficial and systemic infections, especially in immunocompromised individuals. Considering the sharp increase in the rate of Candida infections, and resistance to commonly used antifungal agents in the last decades; this study was conducted to determine the rate of resistance among clinical isolates of Candida species, and to characterize some of the resistant genes among resistant isolates collected in Khartoum. Methods: This is a cross-sectional laboratory-based study included 100 pre-screened Candida species isolates from Khartoum state hospitals. Chromogenic media was used for Candida isolation and/or identification. The standard disc diffusion method was performed to investigate the susceptibility to fluconazole, itraconazole, and amphotericin. Following genomic DNA extraction, the entire ERG11 gene was amplified from some C. albicans resistant isolates, sequenced, and further analyzed. Results: Out of 100 clinical isolates collected, 51% were C. albicans, followed by C. glabrata (31%), C. krusie (8%), C. tropicals (5%), and C. dupliniens (5%). Resistance rate was 23% for fluconazole, 4% for itraconazole, while there were no amphotericin resistant isolates detected. C. albicans ERG11 gene sequence reveals 15 different mutations. Among these, three (D116E, E266D, and V488I) were missense mutations; however, these substitutions do not contribute to fluconazole resistance. Conclusion: C. albicans was found to be the most common species. Resistance against fluconazole was observed most frequently; however, mutations in ERG11 are unlikely to be the reason behind fluconazole resistance among these isolates.

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In vitro susceptibility of Candida albicans clinical isolates to eight antifungal agents in Ouagadougou (Burkina Faso)

Cited by 25 publications

References 23 publications

Molecular identification and antifungal susceptibility testing of Candida species isolated from oral lesions in patients with head and neck cancer undergoing radiotherapy

Molecular identification and antifungal susceptibility testing of Candida species isolated from oral lesions in patients with head and neck cancer undergoing radiotherapy

First detection of mutated ERG11 gene in vulvovaginal Candida albicans isolates at Ouagadougou/Burkina Faso

Detection of antifungal drug-resistant and ERG11 gene mutations among clinical isolates of Candida species isolated from Khartoum, Sudan.

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