In vitro susceptibilities of oral pigmented Bacteroides species to trospectomycin and other selected antimicrobial agents

Johnson, Caroline C.; Wendeler, Michael; Levison, Matthew E.

doi:10.1128/aac.34.8.1597

Cited by 12 publications

(9 citation statements)

References 6 publications

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“…It is also interesting to note that tosufloxacin MICs were significantly lower for P-lactamasenegative than for P-lactamase-positive strains. Johnson and coworkers reported similar findings with trospectomycin and tetracycline (21). A permeability barrier to non-,B-lactams may be the mechanism for this phenomenon in enzymeproducing strains.…”

supporting

confidence: 63%

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Susceptibilities of 394 Bacteroides fragilis, non-B. fragilis group Bacteroides species, and Fusobacterium species to newer antimicrobial agents

Appelbaum

Spangler

Jacobs

1991

Antimicrob Agents Chemother

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The susceptibilities of 374 selected I-lactamase-producing gram-negative anaerobes (including 22 cefoxitinresistant strains and 36 strains refractory to the enhancing effect of ,I-lactamase inhibitors) and 20 jI-lactamasenegative strains were tested by agar dilution against selected new' agents. The organisms included 217 Bacteroides fragilis group strains, 137 non-B. fragilis group Bacteroides spp., and 40 fusobacteria. All strains were susceptible to piperacillin-tazobactam, imipenem, and meropenem. For the B. fragilis group, 96% were susceptible to ampicillin-sulbactam, 95% were susceptible to amoxicillin-clavulanate and cefoperazonesulbactam,' 94% were susceptible to tosufloxacin, 91% were susceptible to cefoxitin, 88% were susceptible to trospectomycin, and 73% were susceptible to cefotetan. For the jI-lactamase-positive non-B. fragilis group Bacteroides spp., .94% were susceptible to cefoxitin, amoxicillin-clavulanate, ampicillin-sulbactam, cefoperazone-sulbactam, and trospectomycin, 90% were susceptible to cefotetan, and 85% were susceptible to tosufloxacin (the most resistant strains were B. bivius and B. disiens). For the jI-lactamase-positive fusobacteria, '97% were susceptible to amoxicillin-clavulanate, ampicillin-sulbactam, cefoperazone-sulbactam, trospectomycin, and cefoxitin, 90% were susceptible to cefotetan, and 89% were susceptible to tosufloxacin. All agents showed excellent activity against jI-lactamase-negative strains (for trospectomycin, 95% were susceptible; for all other drugs,'100% were susceptible). Overall, both carbapenems and piperacillin-tazobactam were most active. Amoxicillin-clavulanate, ampicillin-sulbactam, and cefoperazone-sulbactam lacked activity against some cefoxitin-resistant B. fragilis group strains but had excellent activity against other organisms. Tosufloxacin, a new quinolone, had very good activity against B. fragilis group strains (94% susceptible), good activity against other P-lactamase-positive strains (.85% susceptible), and excellent activity against JI-lactamase-negative strains (100% susceptible; MIC for 90% of strains, 0.5 ,ug/ml). Trospectomycin was active against >90% of all strains except for B. fragilis group strains (88% susceptible; MIC for 90% of strains, 32

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supporting

confidence: 63%

“…A permeability barrier to non-,B-lactams may be the mechanism for this phenomenon in enzymeproducing strains. In contrast to the findings of Johnson et al (21), however, we did not observe lower trospectomycin MICs against non-enzyme-producing strains. Additionally, trospectomycin MICs higher than those reported by Jacobus and Tally (20) were found.…”

contrasting

confidence: 57%

Susceptibilities of 394 Bacteroides fragilis, non-B. fragilis group Bacteroides species, and Fusobacterium species to newer antimicrobial agents

Appelbaum

Spangler

Jacobs

1991

Antimicrob Agents Chemother

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“…These findings agree with those of other studies [5][6][7][8]. Strategies have been developed for the use of antibiotics in periodontal and other infections caused by black-pigmented Gram-negative anaerobes [18,19]. A continued search for such correlations is needed, particularly because plasmids coding for transfer of resistance have been demonstrated in intestinal Bacteroides spp.…”

Section: Discussionsupporting

confidence: 88%

Occurrence of plasmids in black-pigmented Gram-negative anaerobes

HOHNE¹

1993

FEMS Immunology and Medical Microbiology

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Black-pigmented Gram-negative anaerobes are causative agents of pyogenic infections and are closely linked to various forms of periodontal diseases. Whereas many studies have shown a high incidence of plasmids in intestinal Bacteroides spp., there have been only a few reports of plasmid analyses in pigmented Gram-negative anaerobes. According to previous reports and confirmed in this study, plasmids can be present in Porphyromonas asaccharolytica, Prevotella intermedia, Pr. melaninogenica, and B. levii but have not been detected in P. gingivalis or other black-pigmented species. There were no correlations between plasmids and phenotypes such as resistance to antibiotics or bacteriocinogenicity. The highest carriage rate was found in isolates from cases of chronic otitis media, but the relationship between this site of infection and a high incidence of plasmids could be incidental. The size of plasmids ranged from 1.5 to 29 MDa. Plasmids with molecular weight > 10 MDa were described for the first time in these organisms. Repeated plasmid analyses showed that the plasmid patterns were generally stable.

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“…This phenomenon has been reported for tosufloxacin, tetracycline, and trospectomycin and may reflect a generalized permeability barrier in ,-lactamase-producing strains (6,15).…”

mentioning

confidence: 96%

Susceptibilities of 540 anaerobic gram-negative bacilli to amoxicillin, amoxicillin-BRL 42715, amoxicillin-clavulanate, temafloxacin, and clindamycin

Appelbaum

Spangler

Shiman

et al. 1992

Antimicrob Agents Chemother

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Agar dilution MIC testing of amoxicillin, amoxicillin-BRL 42715, amoxicillin-clavulanate, temafloxacin, and clindamycin against 496 ,-lactamase-producing anaerobic gram-negative rods revealed MICs for 90o% of the strains tested of 256.0 (amoxicillin), 2.0 (amoxicillin-BRL 42715 and amoxicillin-clavulanate), and 4.0 (temafloxacin and clindamycin) ,ug/ml. Amoxicillin, temafloxacin, and clindamycin inhibited all 44 13-lactamase-negative strains (MICs for 90%o of the strains tested, <2.0 ,ug/ml). BRL 42715 will not be developed, but temafloxacin merits clinical evaluation.,B-Lactamase production and concomitant resistance to many ,B-lactams are usually encountered in the Bacteroides fragilis group but have been increasingly reported in other anaerobic gram-negative rods (2,6,7,13,14). We have examined the susceptibilities of 304 B. fragilis strains; 171 non-B. fragilis group Bacteroides, Prevotella, and Porphyromonas species (142 were P-lactamase positive); and 65 Fusobacterium species (50 were 1-lactamase positive) to amoxicillin, amoxicillin-BRL 42715 (a potent new ,B-lactamase inhibitor [8,9,17,23]), amoxicillin-clavulanate, temafloxacin (10,11,16,19), and clindamycin.Organisms were selected from clinical isolates collected over the past 4 years from diverse sources as previously described (2-6, 13, 14). Prior to being tested, strains were identified (1, 12, 20) as described previously. P-Lactamase production was tested by the nitrocefin disk method (BBL Microbiology Systems, Cockeysville, Md.) (4). MICs of amoxicillin, amoxicillin-clavulanate, amoxicillin-BRL 42715 (SmithKline Beecham Pharmaceuticals, Brockham Park, Betchworth, United Kingdom), temafloxacin (Abbott Laboratories, Chicago, Ill.), and clindamycin (The Upjohn Co., Kalamazoo, Mich.) were determined by agar dilution (2) extracts were standardized to contain similar units of activity against nitrocefin.In Tables 1 and 2, percent susceptibility rates of amoxicillin with and without inhibitors are presented at breakpoints of 2.0, 4.0, and 8.0 ,ugIml, respectively. Susceptibility data for ,B-lactamase-producing strains are shown in Table 1. Against all 3-lactamase-positive strains, addition of BRL 42715 to amoxicillin lowered the MICs for 50 and 90% of the strains tested (MlC50 and MIC90, respectively) from 32 and 256 to 0.25 and 2 ,ug/ml, respectively, and 99% of the strains were susceptible at all three amoxicillin breakpoints (27% of the strains were susceptible to amoxicillin at the NCCLS breakpoint of 4 ,ug/ml). Addition of clavulanate to amoxicillin lowered the MIC50 and MIC90 to 0.5 and 2.0 ,ug/ml, respectively, with 97% of the strains susceptible at the NCCLS amoxicillin breakpoint of 8 ,ug/ml (18). Temafloxacin and clindamycin showed good activity against 13-lactamase-producing strains (95 and 98%, respectively, were susceptible at .4 ,ug/ml), but clustering near the breakpoint was seen with both compounds.All ,B-lactamase-negative strains were susceptible to amoxicillin, temafloxacin, and clindamycin (Table 2), and many were susceptible to the...

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In vitro susceptibilities of oral pigmented Bacteroides species to trospectomycin and other selected antimicrobial agents

Cited by 12 publications

References 6 publications

Susceptibilities of 394 Bacteroides fragilis, non-B. fragilis group Bacteroides species, and Fusobacterium species to newer antimicrobial agents

Susceptibilities of 394 Bacteroides fragilis, non-B. fragilis group Bacteroides species, and Fusobacterium species to newer antimicrobial agents

Occurrence of plasmids in black-pigmented Gram-negative anaerobes

Susceptibilities of 540 anaerobic gram-negative bacilli to amoxicillin, amoxicillin-BRL 42715, amoxicillin-clavulanate, temafloxacin, and clindamycin

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