In vitro susceptibilities of field isolates of Mycoplasma agalactiae

Antunes, Nuno T.; Tavío, María M.; Assunção, P.; Rosales, Rubén S.; Poveda, Carlos; Fe, Christian de la; Gil, María Concepción Robles; Poveda, José B.

doi:10.1016/j.tvjl.2007.05.008

Cited by 24 publications

(22 citation statements)

References 9 publications

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“…; Antunes et al . ). Although other mycoplasmas have been reported to be susceptible, all strains of Myc.…”

Section: Discussionmentioning

confidence: 97%

“…agalactiae in the present study were resistant, which is in agreement with the work of Antunes et al . (). In contrast to the results of previous studies, isolates in this study presented much higher oxytetracycline MIC values and slightly higher MIC values in the case of tylosin, although Loria et al .…”

Section: Discussionmentioning

confidence: 97%

“…Spectinomycin MIC values were similar to those of Antunes et al . (). A relatively variable response was seen to tulathromycin, which belongs to a group of new‐generation macrolides, and depended on the strain (MIC range between 1 and 8 μg ml −1 ) giving higher MIC 50 and MIC 90 values compared with other antimicrobials of the same family.…”

Section: Discussionmentioning

confidence: 97%

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Isolation, molecular characterization and antimicrobial susceptibilities of isolates of Mycoplasma agalactiae from bulk tank milk in an endemic area of Spain

et al. 2013

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Aim: To isolate and characterize strains of Mycoplasma agalactiae from bulk tank and silo ewes' milk. Methods and Results: Thirteen mycoplasma isolates were obtained from samples of sheep milk taken from bulk tank and large silos and identified as Myc. agalactiae by PCR-DGGE. The isolates were typed by pulsed field gel electrophoresis (PFGE), SDS-PAGE and immunoblot. The in vitro activity of 13 antimicrobials of veterinary interest was tested against these isolates. Results showed that the most effective compounds against Myc. agalactiae in vitro were clindamycin, an antibiotic not previously described as a suitable contagious agalactia (CA) treatment, with Minimum Inhibitory Concentration (MIC) values of <0Á12 lg ml À1 , and quinolones, with MIC values <0Á12-0Á5 lg ml À1 , which are used as standard treatments against CA.Conclusions: Based on the in vitro assay, clindamycin, quinolones, tylosin and tilmicosin would be appropriate antimicrobials for CA treatment. The isolates were mostly resistant to erythromycin, indicating that it would not be a suitable choice for therapy. The isolates showed common molecular and protein profiles by PFGE and SDS-PAGE, with minor differences observed by immunoblot analysis, suggesting a clonal relationship among them. Significance and impact of the Study: This study demonstrated the importance of the appropriate selection of antimicrobials for treatment of CA.

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“…; Antunes et al . ). Although other mycoplasmas have been reported to be susceptible, all strains of Myc.…”

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 97%

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Isolation, molecular characterization and antimicrobial susceptibilities of isolates of Mycoplasma agalactiae from bulk tank milk in an endemic area of Spain

et al. 2013

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“…have been, however, increasingly documented. Indeed, the ewe and goat field isolates were mostly resistant to erythromycin, indicating that this macrolide would not be a suitable choice for therapy (Antunes et al, 2008;de Garnica et al, 2013c;Paterna et al, 2013). In addition, in a recent published study carried out over the last 25 years in M. agalactiae isolates collected from sheep and goats in France, this species showed increased minimum inhibitory concentrations over time for several antimicrobials used against CA (Poumarat et al, 2016).…”

Section: Mycoplasma Sppmentioning

confidence: 99%

Milk hygiene in small ruminants: A review

Gonzalo

2018

Span. j. agric. res.

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Somatic cell count (SCC), mammary pathogens prevalence, total and specific bacterial counts, antimicrobial residues, macroscopic sediment, water addition, aflatoxins and other contaminants constitute the basis for milk payment-schemes, monitoring and improvement of flock hygiene and health management, and development of analytical surveillance programs in the dairy small ruminants. The present work reviews factors influencing the variation of these variables, including milk analytical methods, storage and preservation, along with management implications during the last two decades. Following farmer and cooperative educational programs, progressive reductions have been reported for total bacterial count and antimicrobial residue occurrence in bulk tank milk. These results were consistent, however, with high values for SCC and specific bacterial populations. Thus, mastitis control programs should be intensified to increase hygiene in milk and economic returns for producers and processors. In addition, the implementation of programs to reduce specific bacterial counts (i.e., psychrotrophs, coliforms, Clostridium spp. spores) and mammary pathogen prevalence (i.e., Staph. aureus, Mycoplasma spp.), as well as the use of combined screening methods for an increased rate of antimicrobial detection, are currently required strategies which are positively valuated by milk processors, industry and consumers. Other contaminants may also be present, but cost-effective screening and analytical systems have not yet been implemented. This review aims to be helpful for troubleshooting milk quality and safety, developing future premium payment systems and industry quality-standards, optimizing management, on-farm risk traceability systems and consumer acceptance.

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“…capri (Antunes et al, 2007a). In vivo resistance to other antimicrobial agents involved in the contagious agalactia syndrome has been reported in M. putrefaciens and M. agalactiae (Antunes et al, 2007b(Antunes et al, , 2008; to date, no resistance to fluoroquinolones has been reported (Loria et al, 2003;Antunes et al, 2007aAntunes et al, , 2008. However, considering the increasing reports of quinolone resistance in other animal mycoplasma species such as M. agalactiae and M. bovis Sulyok et al, 2014), it is more than likely that resistance to fluoroquinolones in M. mycoides subsp.…”

Section: Introductionmentioning

confidence: 99%