In vitro supplementation with unsaturated fatty acids improves boar sperm viability after storage at 6 °C

Jakop, Ulrike; Svetlichnyy, Valentin; Schiller, Jürgen; Schulze, Martin; Schröter, Filip; Mueller, Karin

doi:10.1016/j.anireprosci.2019.05.008

Cited by 18 publications

(10 citation statements)

References 34 publications

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“…In frozen-thawed boar semen, it was demonstrated that DHA improved motility, membrane integrity, and acrosome integrity in boar sperm when used alone and in combination with l-cysteine in a freezing extender (Chanapiwat et al 2009;Kaeoket et al 2010c). Recently, Jakop et al (2019) found that the addition of unsaturated fatty acids (linolenic, linoleic, oleic, palmitoleic acid) improved the boar sperm viability of cold stored semen at 6 °C for seven days. The n-3 PUFAs including α-linoleic acid, docosahexaenoic acid and eicosapentaenoic acid act directly on the sperm membrane lipid composition to promote permeability, fluidity and fusogenicity characteristics which are related to the sperm plasma membrane integrity, sperm motility and sperm survival rate (Tran et al 2017).…”

Section: Resultsmentioning

confidence: 99%

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l-cysteine prolonged fresh boar semen qualities, but not for docosahexaenoic acid

Chanapiwat¹,

Kaeoket²

2021

CZECH J ANIM SCI

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The present study aimed to develop a long-term fresh boar semen extender by adding l-cysteine and docosahexaenoic acid (DHA) into Beltsville thawing solution (BTS)-based extender. Semen samples were collected from six boars, diluted at a concentration of 3 × 109 spermatozoa/100 ml and allocated into 10 groups as follows: BTS, Merck III®, Androstar® Plus, ModenaTM, DHA 1.5 mg/ml, 3 mg/ml, and 6.25 mg/ml, l-cysteine 2.5 mM, 5.0 mM, and 10 mM. All extended semen samples were stored at 18 °C and were evaluated for progressive motility, viability, acrosome integrity, pH, and osmolality on days 0, 1, 3, 5, and 7 of storage. The results documented that on day 3, l-cysteine at 2.5 mM showed the significantly highest percentage (78.8%) of progressive motility, but not different from ModenaTM (78.8%) and Androstar® Plus (71.3%) (P < 0.05). On day 5, l-cysteine at 2.5 mM maintained progressive motility and viability up to 73.8% and 77.9%, respectively, but not different from ModenaTM (78.8% and 74.8%) and Androstar® Plus (70.0% and 76.8%) (P < 0.05). On day 7, superior progressive motility (71.3%, 77.5% and 72.5%) and viability (72.8%, 77.3% and 69.8%) were found for l-cysteine at 2.5 mM, ModenaTM and Androstar® Plus groups, respectively, when compared to the other groups (P < 0.01). However, in the present study, DHA supplementation failed to maintain fresh boar semen qualities during storage. In conclusion, adding l-cysteine at a concentration of 2.5 mM in a BTS-based extender is the optimal concentration for the preservation of fresh boar semen at 18 °C for seven days.

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Section: Resultsmentioning

confidence: 99%

“…https://doi.org/10.17221/199/2020-CJAS (Tran et al 2017;Jakop et al 2019). In principle, using a combination of PUFAs and antioxidants with their optimal concentration could be beneficial for the improvement of the boar sperm quality during low-temperature preservation of sperm.…”

Section: Discussionmentioning

confidence: 99%

l-cysteine prolonged fresh boar semen qualities, but not for docosahexaenoic acid

Chanapiwat¹,

Kaeoket²

2021

CZECH J ANIM SCI

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“…Boar sperm are more prone to cold shock than sperm of many other species because of their relatively low concentration of membrane stabilizing cholesterol. However, the susceptibility for cold shock is dependent on many different factors, and recently there have been reports of successfully sustained sperm quality at low temperature through optimization of surrounding conditions 15 , 35 , 36 . Amongst others, boar individual eligibility 37 , 38 , the cooling rate 15 , 39 , the used extender 40 , motionless storage 41 and transport 42 play important roles in the maintenance of sperm quality.…”

Section: Discussionmentioning

confidence: 99%

Low temperature preservation of porcine semen: influence of short antimicrobial lipopeptides on sperm quality and bacterial load

Hensel¹,

Jakop²,

Scheinpflug

et al. 2020

Sci Rep

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Antimicrobial resistance is a steadily increasing problem and poses a serious threat to global public health. Therefore, it is highly necessary to advance the development of novel antimicrobial compounds and semen preservation strategies. The aim of this study was to evaluate a low temperature, antibiotic-free preservation procedure using Androstar Premium (ASP) extender (Minitüb) with antimicrobial lipopeptides. Firstly, seven lipopeptides in two concentrations (1 × minimum inhibitory concentration (MIC)/2 × MIC) were tested on their sperm-compatibility at 17 °C. Two lipopeptides, C16-KKK-NH 2 and C16-KKKK-NH 2 , did not negatively affect sperm quality and were further evaluated for their efficiency of bacterial growth inhibition at 5 °C. Besides an overall diminution of colony forming units, both peptides showed a reduction of bacterial subcultures (n = 103) with a decrement in Gram-positive rods from 65 (ASP w/o supplements) to 39/52 (ASP w/ C16-KKK-NH 2 /C16-KKKK-NH 2), in Gram-positive cocci from 21 to 9/10 and in Gram-negative species from 17 to 8/5 total subcultures. Furthermore, lipopeptides revealed activity towards selected bacteria of potential concern in artificial insemination like Trueperella pyogenes, Alcaligenes faecalis, Pseudomonas aeruginosa (not C16-KKK-NH 2), Pasteurella sp., Providencia stuartii, Escherichia coli (not C16-KKKK-NH 2) and Streptococcus porcinus (not C16-KKKK-NH 2). Consequently, both tested lipopeptides are promising candidates for alternative antibiotic-free preservation techniques of boar semen. Spreading antimicrobial resistance is a growing problem in human and veterinary medicine, poses a serious threat to global public health 1 and should therefore be addressed in a multifaceted approach from all areas of concern, including the industry branch of artificial insemination (AI) in pigs. It has been proven, that unhindered bacterial growth has detrimental effects on several sperm quality characteristics 2 and a transmission of potential pathogens to the inseminated sow has to be prevented in order to minimize negative effects on fertility and litter size 3. Semen collection is a non-sterile process and AI doses are conventionally stored at temperatures between 16 and 18 °C to prevent cold shock injuries to the highly sensitive boar spermatozoa 4. The combination of moderate temperatures and the use of nutrient-rich semen extenders promotes bacterial growth of psychrophilic and mesophilic species 5. As a countermeasure, antibiotics like gentamicin sulphate are routinely added to boar semen preserved for AI (Council Directive, European Union, 90/429/EEC). A steadily growing portion of contaminant bacteria isolated from AI doses are resistant to antibiotics commonly used as additives in semen extenders 6 , threatening animal welfare. This has far-reaching consequences for public health because of the natural backflow of parts of the rather large-volumed AI doses after insemination 7 ,

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“…Similar to the results, 0.5 mmol/L PTA supplementation in ram extender increased the percentage of sperm forward motility, increased total antioxidant enzyme activity at 24, 48 and 72 h, and decreased sperm at 72 h storage of lipid peroxidation, when supplemented at concentrations of 0.25, 0.5 and 1 mmol/L PTA increased SOD activity in sperm and decreased NO production in sperm at 48 and 72 h during refrigeration ( 103 ). Addition of PTA to boar extender increased sperm viability and motility after 2 and 7 days of storage at 6°C, and increased sperm counts with active mitochondria after 3 days of storage ( 104 ). It is suggested that PTA can be added to the extender as an exogenous antioxidant.…”

Section: Effect Of Fas In Extender On Semen Qualitymentioning

confidence: 99%

Regulation of semen quality by fatty acids in diets, extender, and semen

Yuan

Wang

2023

Front. Vet. Sci.

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Fatty acids (FAs) are classified into different types according to the degree of hydrocarbon chain saturation, including saturated fatty acids (SFAs), monounsaturated fatty acids (MUFAs), omega-3 polyunsaturated fatty acids (omega-3 PUFAs) and omega-6 polyunsaturated fatty acids (omega-6 PUFAs), which play an important role in maintaining semen quality. This review focuses on the regulation of FAs in semen, diet and extender on semen quality, and expounds its effects on sperm motility, plasma membrane integrity, DNA integrity, hormone content, and antioxidant capacity. It can be concluded that there are species differences in the FAs profile and requirements in sperm, and their ability to regulate semen quality is also affected by the addition methods or dosages. Future research directions should focus on analyzing the FAs profiles of different species or different periods of the same species and exploring suitable addition methods, doses and mechanism of regulating semen quality.

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In vitro supplementation with unsaturated fatty acids improves boar sperm viability after storage at 6 °C

Cited by 18 publications

References 34 publications

l-cysteine prolonged fresh boar semen qualities, but not for docosahexaenoic acid

l-cysteine prolonged fresh boar semen qualities, but not for docosahexaenoic acid

Low temperature preservation of porcine semen: influence of short antimicrobial lipopeptides on sperm quality and bacterial load

Regulation of semen quality by fatty acids in diets, extender, and semen

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