In vitro supplementation of testosterone or prolactin affects spermatozoa freezability in small ruminants

Martínez-Fresneda, Lucía; O’Brien, E.; Sebastián, Antonio López; Velázquez, Rosario; Toledano-Dı́az, A.; Tesfaye, Dawit; Schellander, K.; García–Vázquez, Francisco Alberto; Santiago-Moreno, J.

doi:10.1016/j.domaniend.2019.06.004

Cited by 11 publications

(8 citation statements)

References 69 publications

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“…Certainly, testosterone can induce variations in the properties of sperm membranes, 49 and the negative effects on CR values of high concentrations might be due to changes in membrane fluidity and susceptibility to glycerol toxicity. 20 In rams, in vitro testosterone supplementation has been reported to reduce frozen-thawed acrosome integrity, 50 but in the present work, neither treatment modified the CR value for this variable.…”

Section: Reproducontrasting

confidence: 59%

Influence of circulating testosterone concentration on sperm cryoresistance: The ibex as an experimental model

Bóveda¹,

Esteso²,

Velázquez³

et al. 2021

Andrology

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Background: Recent studies have noted that the circulating testosterone concentration may affect the ability of spermatozoa to survive cryopreservation. However, few attempts to confirm such a relationship have been made. Wild ruminant species have very marked seasonal changes in their reproductive function and strong annual changes in their plasma testosterone concentration. Objectives:The present work examines the influence of induced changes in testosterone secretion on sperm variables following conventional slow freezing and ultrarapid freezing, using the Iberian ibex as an experimental model. Materials and Methods:In a first experiment, testosterone levels were reduced in the middle of the rutting season (December) using the antiandrogen cyproterone acetate (CA). In a second experiment, testosterone levels were increased at the end of the rutting season (January) via the use of the androgen testosterone propionate (TP).Results: During December, the testosterone concentration was found to be higher in the blood and seminal plasma of untreated males than in those of CA-treated males (p < 0.001 and p < 0.05, respectively). Compared with controls, the TP-treated animals had higher blood plasma testosterone concentrations but lower seminal plasma testosterone concentrations during January (p < 0.01 and p < 0.001, respectively).The seminal vesicles of the TP-treated males were larger than those of untreated males (p < 0.05). When CA was administered, sperm viability improved compared with controls (p < 0.05), irrespective of the freezing protocol followed. For the ultrarapid freezing procedure, the cryoresistance ratio for motility decreased when TP was administered (p < 0.05). The values for fresh sperm morphometric variables decreased during the 50 days after the end of CA treatment (p < 0.001) and increased over the same time after the end of TP treatment (p < 0.001). Discussion and Conclusion:The circulating testosterone concentration appears to influence sperm cryoresistance. This may explain the seasonal changes seen in sperm freezability in some species, independent of fresh sperm quality.

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Section: Reproducontrasting

confidence: 59%

Influence of circulating testosterone concentration on sperm cryoresistance: The ibex as an experimental model

Bóveda¹,

Esteso²,

Velázquez³

et al. 2021

Andrology

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“…In a series of recent experiments, it was demonstrated that in vitro incubation of small ruminants spermatozoa in a testosterone medium leads to a decrease in the acrosome integrity in thawed sperm. The authors of the study also suggested that testosterone negatively affects the resistance of sperm to cryopreservation and its destabilizing effect directly depends on the concentration in vivo (Martínez-Fresneda et al 2019).…”

Section: Resultsmentioning

confidence: 99%

“…The proportion of spermatozoa that survive after freezing-thawing is affected by their susceptibility to cold shock, cooling rate, diluent composition, and osmotic stress, and the functional state of spermatozoa after cryopreservation depend on the action of oxidative stress, membrane stability, membrane receptor integrity, and nuclear structure state (Watson 2000;Elkina et al 2011). Various biologically active compounds can also influence sperm quality and cryostability, among which steroid hormones play a special role (Atroshchenko et al 2019c;Martínez-Fresneda et al 2019;Coloma et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Effect of Testosterone, Dihydrotestosterone, Estradiol and Cortisol on the Quality of Fresh and Cryopreserved Stallion Sperm

Shitikova

Atroshchenko

Krokhotina³

et al. 2022

J Exp Bio & Ag Sci

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The effect of steroid hormones on the quality of fresh and cryopreserve sperm has not been fully understood. This study aimed to evaluate the effect of testosterone, dihydrotestosterone, estradiol, and cortisol on the quality of fresh and cryopreserved stallion sperm. The study was conducted on 40 Equus caballus stallions, including Arab (n=20), Oryol trotting (n=4), Standardbred (n=4), and Soviet Heavy Draft (n=12) breeds. The average age of the experimental animals was 9.9 ± 0.7 years. We determined standard quality indicators in fresh and cryopreserved sperm and the concentration of steroid hormones in the blood plasma of stallions. Results of the study suggested a negative correlation between the level of testosterone with total (r=-0.41; p<0.01) and progressive (r=-0.44; p<0.01) sperm motility in cryopreserved sperm as well as in fresh sperm (r=-0.38; p<0.05 and r=-0.39; p<0.05 correspondingly). While the level of estradiol showed a positive correlation with survival rate in cryopreserved (r=0.35; p<0.05) and in fresh (r=0.33; p<0.05) sperm. Further, the level of cortisol in the blood plasma of stallions did not show any statistically significant correlations with the qualitative characteristics of sperm. A positive relationship was found between the concentration of dihydrotestosterone with the volume of ejaculate (r=0.37; p<0.05) and the total number of sperm in the ejaculate (r=0.43; p<0.01). Results of the study can be concluded that steroid hormones have different effects on the quality indicators of fresh and cryopreserved sperm of stallions and their concentration in the blood should be considered when selecting stallions for cryopreservation of sperm.

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“…The in vitro fertilization experiment can intuitively reflect the fertilization ability of sperm. The cleavage and blastocyst stages are important periods of embryonic development ( 28 ). By comparing the cleavage and blastocyst rates with 2 mmol/L glutathione and without glutathione, it can be seen from the comparison that the addition of 2 mmol/L glutathione improves the sperm fertilization ability.…”

Section: Discussionmentioning

confidence: 99%

Effect of Glutathione on Sperm Quality in Guanzhong Dairy Goat Sperm During Cryopreservation

Zou

Wei²,

et al. 2021

Front. Vet. Sci.

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In the process of cryopreservation of dairy goat semen, it will face many threats such as oxidative damage, which will affect the motility and plasma membrane function of sperm. As an endogenous antioxidant in animals, glutathione (GSH) can significantly improve the quality of thawed sperm when added to the frozen diluent of semen of pigs and cattle. In this study, different concentration gradients of GSH [0 mmol/L (control), 1, 2, 3, 4 mmol/L] were added to the frozen diluent of Guanzhong dairy goat semen. By detecting the sperm motility parameters, acrosome intact rate and plasma membrane intact rate after thawing, the effect of GSH on the cryopreservation of dairy goat semen was explored. Sperm motility parameters were measured with the computer-aided sperm analysis (CASA) system (total power, TM; forward power, PM; linearity, LIN; average path speed, VAP; straight line speed, VSL; curve speed, VCL; beat cross frequency, BCF). The sperm acrosome integrity rate after thawing was detected by a specific fluorescent probe (isothiocyanate-labeled peanut agglutinin, FITC-PNA), and the sperm plasma membrane integrity rate after thawing was detected by the hypotonic sperm swelling (HOST) method. Reactive oxygen species (ROS) kit, malondialdehyde (MDA) kit, superoxide dismutase (SOD) kit, glutathione peroxidase (GSH-PX) kit were used to detect various antioxidant indicators of thawed sperm. in vitro fertilization experiment was used to verify the effect of adding glutathione on sperm fertilization and embryo development. The results showed that when the concentration of glutathione was 2 mmol/l, the sperm viability, plasma membrane intact rate, and acrosome intact rate were the highest after thawing, reaching 62.14, 37.62, and 70.87% respectively, and they were all significantly higher. In terms of antioxidant indexes; the values of SOD and GSH-PX were 212.60 U/ml and 125.04 U/L, respectively, which were significantly higher than those of the control group; The values of ROS and MDA were 363.05 U/ml and 7.02 nmol/L, respectively, which were significantly lower than the control group. The addition of 2 mmol/L glutathione significantly improves the fertilization ability of sperm. In short, adding 2 mmol/l glutathione to the semen diluent can improve the quality of frozen Guanzhong dairy goat sperm.

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In vitro supplementation of testosterone or prolactin affects spermatozoa freezability in small ruminants

Cited by 11 publications

References 69 publications

Influence of circulating testosterone concentration on sperm cryoresistance: The ibex as an experimental model

Influence of circulating testosterone concentration on sperm cryoresistance: The ibex as an experimental model

Effect of Testosterone, Dihydrotestosterone, Estradiol and Cortisol on the Quality of Fresh and Cryopreserved Stallion Sperm

Effect of Glutathione on Sperm Quality in Guanzhong Dairy Goat Sperm During Cryopreservation

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