In Vitro Study on the Biological Characteristics of Adipose-Derived Stem Cells from Liposuction Area in Patients with Progressive Hemifacial Atrophy

Gao, Qiuni; Qi, Zuoliang; Jin, Xiaolei; Yang, Zhenyu; Yuan, Xihang; Hu, Yuling; Yang, Xiaonan

doi:10.1007/s00266-022-02902-x

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“…A highly significant up‐regulation of BAX ( p < 0.001) occurred in PHA‐ADSCs(Figure 9B). The results were consistent with those obtained in vitro 11 (Figure 9A).…”

Section: Resultssupporting

confidence: 93%

Biological characteristics of adipose‐derived stem cells from patients with progressive hemifacial atrophy: An in vivo study

Gao

Jin

et al. 2022

J of Cosmetic Dermatology

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Objective: In this study, we investigate the biological characteristics of ADSCs from patients with progressive hemifacial atrophy (PHA) in vivo and try to explore the theoretical support for cell-assisted lipotransfer in treating patients with PHA.Methods: ADSCs and exosomes were respectively extracted from patients with PHA and healthy ones. PHA-ADSC was detected the differentiation ability, phenotype, and anoxic resistance. NTA particle size, electron microscopy (TEM), and WB for CD63 and TSG10 were used to detect the exosomes. ADSCs of PHA (PHA-ADSCs) and healthy ones (NORM-ADSCs) mixed with their granular fat, exosomes mixed with PHA-granular fat, and PBS mixed with PHA-granular fat as the control group. The four groups of different grafts were, respectively, transplanted into nude mice, and the fat grafts were dissected and weighed at 2, 4, 8 and 12 w. Weight and volume retention were calculated for each of the four groups. Then, the four groups of fat grafts were tested for hematoxylin-eosin (HE) and immunohistochemical stainings, CD31 for blood vessel formation, CD68 for macrophage infiltration, and perilipin for fat formation, RT-PCR analysis of the APRC5, ATG5, ATG7, ATG12, BAX, PPARG, CDKN1A, and CDKN2A genes.Results: ADSCs in the PHA group had typical phenotypes and multidirectional abilities. The PHA-ADSCs -assisted lipotransfer group, exhibited a weaker droplet formation and lower volume retention rate than the NORM-ADSCs-assisted lipotransfer group but much better than the non-cell-assisted lipotransfer group. Exosomeassisted lipotransfer group showed benefits, too. Conclusions:The PHA-ADSCs-assisted lipotransfer and the exosome-assisted lipotransfer improved the fat survival rate after fat filling in patients with hemifacial atrophy. Cell-assisted and exosome-assisted lipotransfer is an effective method to treat hemifacial atrophy.

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“…A highly significant up‐regulation of BAX ( p < 0.001) occurred in PHA‐ADSCs(Figure 9B). The results were consistent with those obtained in vitro 11 (Figure 9A).…”

Section: Resultssupporting

confidence: 93%