In vitro study on chondrogenic differentiation of human adipose-derived stem cells on treated bovine pericardium

Abstract

Bovine pericardium has been proposed as an available material for tissue engineering and bioprosthetic reconstruction. In this study, bovine pericardium was fabricated into a scaffold for culturing and chondrogenic differentiation of human adipose-derived stem cells (hADSCs). Bovine pericardium was treated in 10 mM Tris-HCl and 0.15% SDS, followed by crosslinking in 0.1% glutaraldehyde. Treated bovine pericardium (tBP) was characterized as a slight yellowish thin membrane with enhanced tensile strength and strain property. The membrane maintained stability under enzymatic conditions for up to 16 days of incubation. The results confirmed tBP as a cell-friendly scaffold for hADSCs due to low cytotoxicity and its ability to support an appropriate attachment and proliferation of hADSCs. Moreover, there was an accumulation of the extracellular matrix proteoglycan in tBP seeded with hADSCs after 7 and 14 days of chondrogenic induction. COMP as a specific marker of chondrogenesis was detected after 7 days, whereas type X-a1 collagen (Col10a1) expression was stable up to day 14. However, minor expression of aggrecan was found. Taken together, these results indicate that tBP is a potential scaffold for hADSCs for cartilage tissue engineering.Key words: Bovine pericardium, scaffold, adipose-derived stem cells, chondrogenic differentiation, cartilage regeneration, augmentation rhinoplasty

Repair of articular cartilage defect using adipose‐derived stem cell‐loaded scaffold derived from native cartilage extracellular matrix

“…Moreover, many researchers have successfully induced chondrogenic differentiation of ADSCs in vitro and in vivo (Goudarzi et al, 2018;Hwang, Noh, Hong, & Lee, 2020;Yang, Chen, Yang, Hsiao, & Wang, 2020). And in the field of cartilage tissue engineering, an increasing number of studies have confirmed that adipose stem cells have good chondrogenic differentiation ability, which is comparable to bone marrow stromal stem cells (Chung et al, 2019;Keshavarz, Jalili, Pazhouhi, & Khazaei, 2019;Nguyen, Doan, & Tran, 2019). Therefore, ADSCs are cartilage tissue engineering seed cells, with rare advantages and prospects.…”

Đặc Tính Sinh Học Và Che Phủ Khuyết Hổng Xương Của Màng Tim Heo Vô Bào Trong Điều Kiện in Vitro

“…Màng được tạo ra bằng cách loại bỏ hoàn toàn thành phần tế bào trong màng tim heo bằng phương pháp khử tế bào. Màng tim khử tế bào (dPP) được chứng minh là có cấu trúc collagen đặc, tính tương hợp sinh học, không gây độc cho tế bào, khả năng hỗ trợ tế bào tăng sinh tốt [10], [9]. Ngoài ra, việc sản xuất màng dPP hoàn toàn có thể tiến hành ở quy mô công nghiệp với giá thành rẻ vì đây làm sản phẩm thải trong chăn nuôi heo.…”