In vitro study on antidiabetic and antihypertensive activities of ethanolic extract of propolis of Indonesian stingless bee Tetragonula sapiens

Farida, Siti; Pratami, Diah Kartika; Sahlan, Muhamad; Mun’im, Abdul; Djamil, Ratna; Winarti, Wiwi; Ayub, Rashid; Alahmadi, Tahani Awad; Rahmawati, Siti Irma; Putra, Masteria Yunovilsa; Bayu, Asep; Iqbal, Muhammad Adnan

doi:10.1016/j.jksus.2023.102738

Cited by 5 publications

(4 citation statements)

References 34 publications

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“…All the studied Gerês propolis samples exhibited ash and wax levels below the maximum values specified in the literature for Portuguese/European propolis (2% and 25%, respectively) [21], as well as those permitted by the Technical Regulation of Propolis Identity and Quality (TRPIQ) (5% for ash and 25% for wax contents) [17]. This is a desirable condition since a higher ash content may suggest the presence of inorganic impurities or contamination [34]. In addition, high levels of waxes can adversely affect propolis' purity and quality and could indicate poor processing or contamination [35,36].…”

Section: Discussionmentioning

confidence: 95%

“…Therefore, the TPC and TFC values presented by Gerês propolis indicate a promising antioxidant activity for these extracts. Ortho-diphenols are a recently evaluated parameter in propolis [34]; though, they are extremely relevant in light of the correlation that appears to exist between their presence and the antioxidant potential of natural products [45,46]. This correlation seems to be valid also in the case of Gerês propolis [31].…”

Section: Discussionmentioning

confidence: 99%

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Examination of Raw Samples and Ethanol Extracts of Gerês Propolis Collected in Different Years

Caetano,

Oliveira,

Pereira

et al. 2023

Plants

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Propolis, a natural resin created by bees, has garnered significant attention from both the scientific community and industry due to an impressive range of bioactivities. Nonetheless, the intrinsic variability in its chemical composition and bioactive profiles has been hindering propolis’ full potential use. We previously showed that ethanol extracts (EEs) of a Portuguese propolis sample (Gerês) collected over four consecutive years displayed similar chemical and biological profiles, a constancy never documented before. However, the characteristics of the unprocessed samples of Gerês propolis were never described. Hence, the central objective of this study is to assess the quality parameters of unprocessed propolis samples collected from Gerês (G), over a four-year period (2019–2022), alongside the analysis of the chemical composition and bioactivities of the EEs prepared with the same raw samples. The ash, wax, balsam and water contents of the unprocessed samples—G19 to G22—showed minor fluctuations, likely attributed to uncontrollable natural events impacting the propolis source and collection process. On the other hand, the antimicrobial and antioxidant activities of all the four ethanol extracts (G19.EE–G22.EE) consistently align with prior studies. Furthermore, the Gerês propolis extracts showed remarkable uniformity in chemical composition parameters too, particularly concerning total polyphenol, flavonoid and ortho-diphenol contents. In summary, our research reinforces the beneficial properties of propolis and show that extracts’ bioactivities remain within the reference ranges for Gerês propolis, despite minor differences in unprocessed samples, suggesting a consistent action over time. Thus, this work could be instrumental towards the establishment of standard parameters for propolis applications, offering valuable insights to this field of propolis research.

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Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

Examination of Raw Samples and Ethanol Extracts of Gerês Propolis Collected in Different Years

Caetano,

Oliveira,

Pereira

et al. 2023

Plants

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“…Alkaloids and flavonoids are known to be components that are widely present in propolis extract [18]. The presence of alkaloids, flavonoids, tannins, and coumarin compounds has been found in propolis from several regions, such as Indonesia [19,20], Malaysia [21], Pakistan [22], and India [4]. Alkaloid compounds have some biological activity such as anti-inflammatory, anticancer, and antibacterial [23].…”

Section: Qualitative Phytochemical Analysismentioning

confidence: 99%

Phytochemical and Bioactivity of Propolis Extracts Produced by Stingless Bee Heterotrigona itama from Two Cultivation Locations

Rusman,

Arung

2023

IOP Conf. Ser.: Earth Environ. Sci.

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Propolis produced by the stingless bee is one of the non-timber forest products that currently has great potential to be developed. This research aimed to analyze the phytochemical present in propolis and to investigate the bioactivities of Heterotrigona itama propolis. Propolis samples were collected from cultivation locations in the Faculty of Forestry at Mulawarman University and cultivation locations at Mangkurawang Sub District at Kutai Kartanegara. Propolis was extracted with ethanol and methanol for a mixture of propolis and olive oil. Phytochemical analysis was carried out qualitatively and quantitatively. Antioxidant activity was performed using the radical scavenging assay against DPPH. The antimicrobial activity was determined using the agar-well diffusion method. The preliminary phytochemical analysis showed the presence of alkaloids, flavonoids, tannins, carbohydrates, and coumarins. The results showed that the highest antioxidant activity was found in propolis ethanol extract from Mangkurawang with an IC50 value of 112.53 μg/ml, followed by ethanol extract from Fahutan with an IC50 of 165.382 μg/ml. Furthermore, the ethanol extract from Mangkurawang showed antimicrobial activity against Staphylococcus epidermidis, Cutibacterium acnes, and Candida albicans. This present study displayed the potential uses of propolis as an antioxidant and antimicrobial agent.

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“…In many countries, propolis is used in traditional medicine, cosmetics, and food industries (Omer et al., 2023 ; Viuda‐Martos et al., 2008 ). It has also many pharmacological activities such as antioxidants (Ding et al., 2021 ), antimicrobial (Abdullah et al., 2020 ), antiamoebic (Zullkiflee et al., 2023 ), antidiabetic, antihypertensive (Farida et al., 2023 ), antiviral (Ożarowski & Karpiński, 2023 ), anti‐inflammatory (El‐Guendouz et al., 2017 ), anti‐allergic, hepatoprotective (Kolankaya et al., 2002 ), analgesic–anesthetic activity (Orsatti & Sforcin, 2012 ), immuno‐modulatory (Zullkiflee et al., 2022 ), and anticancer properties (Campoccia et al., 2021 ). Propolis is a functional food so many researchers focus on propolis attributing to its biological properties (Busch et al., 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Isolation of phenolic compounds from eco‐friendly white bee propolis: Antioxidant, wound‐healing, and anti‐Alzheimer effects

Necip,

Demirtas,

Tayhan

et al. 2023

Food Science & Nutrition

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This study presents the first findings regarding extraction, isolation, enzyme inhibition, and antioxidant activity. The oral mucosal wound‐healing process was investigated using propolis water extract (PWE) incubation with gingival fibroblast cells and concluded that propolis was effective on the oral mucosal wound‐healing pattern compared to untreated controls. Additionally, phenolic compounds (fraxetin, apigenin, galangin, pinobanksin, chrysin, etc.) were isolated from propolis, and their chemical structures were elucidated using comprehensive spectroscopic methods. The antioxidant and anti‐Alzheimer potential activities of PWE and some isolated compounds were screened and revealing their inhibitory effects on acetylcholinesterase (AChE) with IC50 values ranging from 0.45 ± 0.01 to 1.15 ± 0.03 mM, as well as remarkable free‐radical scavenging and metal reduction capacities. The results suggest that these compounds and PWE can be used as therapeutic agents due to their antioxidant properties and inhibitory potential on AChE. It can also be used for therapeutic purposes since its wound‐healing effect is promising.

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In vitro study on antidiabetic and antihypertensive activities of ethanolic extract of propolis of Indonesian stingless bee Tetragonula sapiens

Cited by 5 publications

References 34 publications

Examination of Raw Samples and Ethanol Extracts of Gerês Propolis Collected in Different Years

Examination of Raw Samples and Ethanol Extracts of Gerês Propolis Collected in Different Years

Phytochemical and Bioactivity of Propolis Extracts Produced by Stingless Bee Heterotrigona itama from Two Cultivation Locations

Isolation of phenolic compounds from eco‐friendly white bee propolis: Antioxidant, wound‐healing, and anti‐Alzheimer effects

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