In vitro study of the effects of cadmium on the activation of the estrogen response element using the YES screen

Denier, Xavier; Couteau, Jérôme; Baudrimont, Magalie; Hill, Elisabeth M.; Rotchell, J.R.; Minier, Christophe

doi:10.1016/j.marenvres.2008.02.038

Cited by 10 publications

(4 citation statements)

References 10 publications

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“…To achieve both quantitatively and qualitatively successful analysis results for EDCs, a combined approach incorporating two types of analyses was proposed, known as a bioassay-directed chemical analysis (BDCA) method [85]. In regards to this type of analysis, sample screening was performed by using a yeast estrogen screen (YES) assay for total estrogenic activity; then, the analysis for specific chemical species was conducted by using LC-MS/MS [85,111]. Another application method was bioassay-directed fractionation, which consists of a cell bioassay (E-SCREEN), in conjunction with acid-base partitioning (F1 and F2) and the silica gel column fractionation of neutral fractions (F3-F7) [112].…”

Section: Liquid Phase Microextraction Methodsmentioning

confidence: 99%

The methods of identification, analysis, and removal of endocrine disrupting compounds (EDCs) in water

Chang

Choo

Lee

et al. 2009

Journal of Hazardous Materials

340

136

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Section: Liquid Phase Microextraction Methodsmentioning

confidence: 99%

The methods of identification, analysis, and removal of endocrine disrupting compounds (EDCs) in water

Chang

Choo

Lee

et al. 2009

Journal of Hazardous Materials

340

136

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“…In males, the vitellogenin gene is normally silent, although it can be activated by estrogenic exposure 20 . It was assumed that the elevation in hepatic vitellogenin in male medaka was caused by the estrogenic activity of cadmium, because cadmium exhibited estrogenic activity 21 , 22 , 23 . On the other hand, estrogenic compounds induce apoptosis of testicular germ cells such as spermatocytes and spermatogonial stem cells 24 , 25 .…”

mentioning

confidence: 99%

Effects of cadmium exposure on medaka (<i>Oryzias latipes</i>) testes

et al. 2017

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Adult male medaka (Oryzias latipes) were exposed to 10 ppm of cadmium for 96 h, and the testes were examined histopathologically. Numerous apoptotic cells were found in the spermatogonia and spermatocytes at 72 and 96 h after initiation of cadmium exposure, and the pyknotic index, TUNEL-positive rate, and cleaved caspase-3-positive rate in the spermatogonia and spermatocytes of the cadmium-treated group were higher compared with the control group. No significant difference between the control and cadmium-treated groups was found in the phospho-histone H3-positive rate in the spermatogonia and spermatocytes. No edematous, hemorrhagic, or necrotic changes were observed within the testes in the cadmium-treated group. These results suggest that spermatogonia and spermatocytes in medaka testes are highly sensitive to cadmium. Exposure to 10 ppm of cadmium induced histopathologic changes in the testes that were similar to those described in rodents exposed to low doses of cadmium.

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“…Since the initial study in 1988, a multitude of different approaches using yeast assays for oestrogenic activity have been published. For example, it has been used to screen animal feed, calf urine and heavy metals for potential oestrogenic activity (Bovee, Bor et al 2006;Denier, Couteau et al 2008;Bovee, Bor et al 2009) as well as to detect SERMs and other chemicals and extracts (Routledge and Sumpter 1996;Gaido, Leonard et al 1997;Takamura-Enya, Ishihara et al 2003;Bovee, Thevis et al 2010).…”

Section: Numerous Other Publications Show Nuclear Receptors Being Ectopically Expressed Inmentioning

confidence: 99%

Kākāpō reproduction: Identification of steroid receptors and oestrogenic activity in native flora

Davis¹

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<p>Kākāpō (Strigops habroptilus) are a critically endangered parrot species endemic to New Zealand that exhibit a reproductive strategy linked to “masting” years. Crucial to their survival is increased reproductive success. It has been hypothesised that their pattern of reproduction is synchronised with a steroidal “trigger” present in plants during intensive masting. If this hypothesis is valid, then Kākāpō and other closely related NZ parrots might be receptive to these masting plants in a manner different from that of other avian species. The aims of this study were firstly, to identify whether unique amino acid motifs were present in the ligand binding domains (LBD) for the steroid receptors oestrogen receptor α (ER-α), oestrogen receptor β (ER-β), androgen receptor (AR) and progesterone receptor (PR) for the New Zealand parrots Kākāpō, Kākā, Kākāriki and Kea. These LBD amino acid sequences were compared with those in an Australian parrot, the Cockatiel, as well as in Chicken and Japanese Quail. Moreover, the role of these amino acid changes on the binding of ligands (both the native ligand and other steroidogenic compounds) for ER-α was assessed by in silico modelling by comparing the most favourable binding position of the ligands in the three-dimensional structure of Kākāpō ER-α with that of human ER-α. The second aim was to test extracts of New Zealand native plants known to be a food source for Kākāpō for their seasonal variation in oestrogenic activity and hence possible involvement in the reproductive cycle of Kākāpō. The LBD for ER-β and AR of the parrot species (New Zealand and Australian) displayed 100% identity whilst those for ER-α and PR had variations. When the parrot sequences were compared with those for other avian species and human, there were a number of amino acid differences present, with the greatest disparity present in the LBD of ER-α. From in silico modelling studies, the amino acid substitutions in this receptor were predicted to have an indirect influence on the binding position of both 17β-oestradiol and a number of phytoestrogens through changes in the structure of this region. Consequently, this could have an effect on the binding affinity of certain plant chemicals for ER-α and thus their potency. Plants extracts from the foliage and fruit of native New Zealand species were screened for oestrogenic activity in a yeast bioassay transfected with the human isoform of ER-α. The results from these experiments indicated that all the plants tested (excluding Dacrycarpus dacrydioides) exhibited detectable oestrogenic activity and a number also displayed variable and sometimes seasonal trends in activity. In summary, the results in this study identified ER-α LBD amino acid motifs unique to New Zealand parrots. For the other steroid receptors, the ER-β LBD had a three amino acid combination of M, V and L that was only present in the parrot species whilst both the AR and PR LBD displayed much greater inter-species conservation. These changes, particularly those in the ER-α LBD, may influence the binding of steroidogenic compounds, known to be present in the plants consumed by Kākāpō as demonstrated by in silico modelling. Thus, this research provides evidence that the diet of Kākāpō may influence their reproductive pattern. However, it remains to be determined whether the steroidogenic potency of these plant extracts is modified when interacting with the unique LBD of native NZ parrots.</p>

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In vitro study of the effects of cadmium on the activation of the estrogen response element using the YES screen

Cited by 10 publications

References 10 publications

The methods of identification, analysis, and removal of endocrine disrupting compounds (EDCs) in water

The methods of identification, analysis, and removal of endocrine disrupting compounds (EDCs) in water

Effects of cadmium exposure on medaka (<i>Oryzias latipes</i>) testes

Kākāpō reproduction: Identification of steroid receptors and oestrogenic activity in native flora

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