In vitro study of the effect of resveratrol purified from the skin of Iraqi black grape (Vitis vinifera) on lymphocyte cultures isolated from the blood of patients with lymphoma

Abstract

The natural stilbene compound resveratrol (RSV) was extracted and purified locally from the black grape skin (Vitis vinifera) cultivated in Iraq. Cultures of human peripheral lymphocytes were obtained from the blood samples of patients with and without lymphoma to be treated with RSV at different concentrations. Three RSV concentration levels were subjected to isolated lymphocytes from blood samples of Hodgkin lymphoma (HL), non-Hodgkin lymphoma (NHL), and without lymphoma to estimate the change in TNF-α and IL-10. Resveratrol seemed to differently affect cytokines level in normal and lymphoma lymphocytes in relation to its concentration. The lowest resveratrol concentration (50 µg/ml) decreased TNF-α levels for patients without lymphoma and all NHL patients in contrast to the HL sample. Treating normal lymphocytes with a higher dose (1000 µg/ml) might elevate the levels of TNF-α in almost all samples. There was an inverse relationship between both cytokines in most treatments; with the increase in TNF-α level, there was a decrease in IL-10 level except in HL and normal lymphocytes treatment. The locally purified resveratrol could serve as a multi-target drug that modulates the immune system to improve body defense in patients suffering from lymphoma and in patients without lymphoma by altering cytokine levels in response to different resveratrol concentrations in a different manner.

Resveratrol as sensitizer in colorectal cancer plasticity

“…Its effects on cancer cells and patients are being studied in vitro , in vivo, and clinically [ 33 – 35 ]. Specifically, this phytopharmaceutical has shown significant immunomodulatory potential [ 36 – 39 ] and immune system-balancing effects concerning tumor necrosis factors (TNFs) and interleukins (ILs) in both healthy and lymphoma patients [ 40 ]. Moreover, Resveratrol is a poly-target agent capable of modifying several cell signaling cascades, selectively exerting cytotoxicity on cancer cells and able to attenuate cell metastasis by suppressing the epithelial-to-mesenchymal transition (EMT) plasticity signaling [ 41 ] and, simultaneously, no toxicity on normal cells [ 42 ].…”