In vitro study of the mesenchymal stem cells‐conditional media role in skin wound healing process: A systematic review

Heydari, Mohammad Bagher; Ghanbari-Movahed, Zahra; Heydari, Maryam; Farzaei, Mohammad Hosein

doi:10.1111/iwj.13796

Cited by 7 publications

(4 citation statements)

References 100 publications

(248 reference statements)

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“…We have previously studied the use of CM in the preservation solution of pancreatic islets to increase beta cell activities [9]. Along with the recent trend in stemcell-based regenerative medicine, reports of efficacies of CM in dermatology, such as in the treatment of damaged skin and alopecia, have increased [10][11][12][13][14]. In these studies, 2 of 10 autologous, allogeneic, or immortalized MSCs were used.…”

Section: Introductionmentioning

confidence: 99%

Proposal of Simplified Standardization of the Cell-Growth-Promoting Activity of Human Adipose Tissue Mesenchymal Stromal Cell Culture Supernatants

Enosawa,

Kobayashi,

Kobayashi

2024

IJMS

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The conditioned medium (CM) obtained from mesenchymal stromal cell (MSC) culture has excellent cell growth-promoting activity and is used for cosmetics and healthcare products. Unlike pharmaceuticals, strict efficacy verification is not legally required for these products. However, their efficacy must be substantiated as commercial products. We attempted to simplify CM production and to standardize the evaluation of the growth-promoting activity of CM. CM was obtained through the culturing of two lines of commercially available human adipose tissue-derived MSCs using MEMα with or without 10% fetal bovine serum (FBS) for 24 h. Non-CM control media were produced by the same protocol without MSCs. Growth-promoting activities of the CM were estimated by [3H]-thymidine pulse. CM were subjected to molecular weight fractionation with ultrafiltration using 10 k-, 30 k-, 50 k-, and 100 k-membranes. The FBS-free CMs showed 1.34- to 1.85-fold increases and FBS-containing CMs showed 1.45- to 1.67-fold increases in proliferation-promoting activity compared with non-CM controls, regardless of the source of the cell. The thymidine incorporation levels were approximately three times higher in FBS-containing CMs. Aged cells also showed 1.67- to 2.48-fold increases in the activity due to FBS-containing CM, but not to FBS-free CM. The CM activities were sustained even after 1 year at 4 °C. Molecular weight fractionation showed that the activity was recovered in the fraction above 100 k. Clear and stable cell-growth-promoting activity was confirmed with CMs of commercially available adipose tissue MSCs. The activity was detected in the fraction over 100 k. We propose here the importance of standardizing the production and evaluation of CMs to indicate their specific action.

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Section: Introductionmentioning

confidence: 99%

Proposal of Simplified Standardization of the Cell-Growth-Promoting Activity of Human Adipose Tissue Mesenchymal Stromal Cell Culture Supernatants

Enosawa,

Kobayashi,

Kobayashi

2024

IJMS

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“…Cell migration, angiogenesis, inflammation, creation of granulation tissue, remodelling of the extracellular matrix (ECM), and re-epithelialisation are all steps in the process of healing a wound [1]. Effective skin wound healing continues to be a major issue for global healthcare, as currently available skin substitutes and alternative therapeutics produce unsatisfactory results, and results vary with wound types caused by traumatic injuries, burns, and diabetes, where delayed healing and scarring are realities.…”

Section: Introductionmentioning

confidence: 99%

Effect of Multiple-Cycle Collections of Conditioned Media from Different Cell Sources towards Fibroblasts in In Vitro Wound Healing Model

Fadilah,

Fauzi,

Maarof

2024

Pharmaceutics

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Conditioned media refers to a collection of the used cell culture media. The goal of this study was to evaluate the possible impacts of different conditioned media collected across a number of cycles on the fibroblast proliferation, migration, and profiles of protein release. Human dermal fibroblast (HDF) cells and Wharton jelly mesenchymal stem cells (WJMSC) were cultured and incubated for 3 days prior to being harvested as cycle-1 using the serum-free media F12:DMEM and DMEM, respectively. The procedures were repeatedly carried out until the fifth cycle of conditioned media collection. An in-vitro scratch assay was conducted to measure the effectiveness of wound healing. Collagen hydrogel was combined separately with both the Wharton jelly-conditioned medium (WJCM) and the dermal fibroblast-conditioned medium (DFCM) in order to evaluate the protein release profile. The conditioned medium from many cycles had a lower level of fibroblast attachment than the control (complete medium); however, the growth rate increased from 100 to 250 h−1, when supplemented with a conditioned medium collected from multiple cycles. The wound scratch assay showed that fibroblast cell migration was significantly increased by repeating cycles up to cycle-5 of DFCM, reaching 98.73 ± 1.11%. This was faster than the rate of migration observed in the cycle-5 of the WJCM group, which was 27.45 ± 5.55%. Collagen hydrogel from multiple cycles of DFCM and WJCM had a similar protein release profile. These findings demonstrate the potential for employing repeated cycles of DFCM- and WJCM-released proteins with collagen hydrogel for applications in wound healing.

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“…[5] First, the lack of clinical application of MSCs could be attributed to their poor quality and the inconsistency of their therapeutic features, such as self-renewal, differentiation immunocompatibility, genomic stability, heterogeneity, and migratory capacity. [6,7] Numerous attempts have been made to overcome these limitations, including the use of a cell-free conditional culture medium [8] or extracellular vesicles such as exosomes. [9] However, due to factors such as low production, lack of standards for quality control and risk of contamination, the application of these methods remains limited.…”

Section: Introductionmentioning

confidence: 99%

Biomimetic Periodontal Ligament Transplantation Activated by Gold Nanoparticles Protects Alveolar Bone

Zong

Holm

Bronckaers

et al. 2023

Adv Healthcare Materials

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Stem cell therapy might be a promising method to stimulate alveolar bone regeneration, which is currently a major clinical challenge. However, its therapeutic features largely depend on pretreatment and transplantation preparation. Herein, a novel biomimetic periodontal ligament transplantation composed of human periodontal ligament stem cells (hPDLSCs) pretreated with gold nanocomplexes (AuNCs) and embedded in a type-I collagen hydrogel scaffold is developed to protect alveolar bone from resorption. AuNCs are readily absorbed by primary hPDLSCs, with limited cytotoxicity, and promote osteogenic differentiation of hPDLSCs effectively in vitro. In addition, the AuNCs-induced hPDLSCs are encapsulated with type-I collagen hydrogel scaffold to mimic their native physiological niche, and then are transplanted into a rat model of alveolar bone resorption. Both micro-computed tomography (micro-CT) and immunohistochemical assays demonstrate that alveolar bone loss is significantly prevented. Furthermore, the underlying therapeutic mechanism is elucidated, in which transplantation-activated osteogenesis is associated with autophagy, which enables bone remodeling and regeneration. This study provides critical insight into the role of PDLSCs in bone homeostasis and proposes an innovative AuNCs-based strategy for stem cell therapy in bone regeneration.

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In vitro study of the mesenchymal stem cells‐conditional media role in skin wound healing process: A systematic review

Cited by 7 publications

References 100 publications

Proposal of Simplified Standardization of the Cell-Growth-Promoting Activity of Human Adipose Tissue Mesenchymal Stromal Cell Culture Supernatants

Proposal of Simplified Standardization of the Cell-Growth-Promoting Activity of Human Adipose Tissue Mesenchymal Stromal Cell Culture Supernatants

Effect of Multiple-Cycle Collections of Conditioned Media from Different Cell Sources towards Fibroblasts in In Vitro Wound Healing Model

Biomimetic Periodontal Ligament Transplantation Activated by Gold Nanoparticles Protects Alveolar Bone

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