In vitro study of the antibacterial properties and microbial colonization susceptibility of four self-etching adhesives used in orthodontics

Jacobo, Carmen; Torrella, F.; González, Luis Alberto Bravo; Ortiz, A. S.; Vicente, Ascensión

doi:10.1093/ejo/cjt032

Cited by 12 publications

(13 citation statements)

References 30 publications

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“…Attempts have been made to develop an adhesive with antimicrobial properties to prevent white spots during orthodontic treatment . The literature agrees that orthodontic adhesives associated with antimicrobial agent has indeed in vitro antimicrobial activity . However, their in vivo effect needs to be sustained.…”

Section: Discussionmentioning

confidence: 99%

“…There has been some research over the last 10 years, investigating the association of antimicrobial agents and orthodontic adhesives . One of these agents is the antimicrobial monomer MDPB (12‐methacryloyloxydodecylpyridinium bromide), which is synthesized by combining the antibacterial agent quaternary ammonium and a methacryloyl group .…”

Section: Introductionmentioning

confidence: 99%

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The effect of antimicrobial agents on bond strength of orthodontic adhesives: a meta‐analysis of in vitro studies

Altmann

Collares

Leitune

et al. 2015

Orthod Craniofacial Res

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Antimicrobial orthodontic adhesives aim to reduce white spot lesions' incidence in orthodontic patients, but they should not jeopardizing its properties. Systematic review and meta-analysis were performed to answer the question whether the association of antimicrobial agents with orthodontic adhesives compromises its mechanical properties and whether there is a superior antimicrobial agent. PubMed and Scopus databases. In vitro studies comparing shear bond strength of conventional photo-activated orthodontic adhesives to antimicrobial photo-activated orthodontic adhesives were considered eligible. Search terms included the following: orthodontics, orthodontic, antimicrobial, antibacterial, bactericidal, adhesive, resin, resin composite, bonding agent, bonding system, and bond strength. The searches yielded 494 citations, which turned into 467 after duplicates were discarded. Titles and abstracts were read and 13 publications were selected for full-text reading. Twelve studies were included in the meta-analysis. The global analysis showed no statistically significant difference between control and experimental groups. In the subgroup analysis, only the chlorhexidine subgroup showed a statistically significant difference, where the control groups had higher bond strength than the experimental groups. Many studies on in vitro orthodontic bond strength fail to report test conditions that could affect their outcomes. The pooled in vitro data suggest that adding an antimicrobial agent to an orthodontic adhesive system does not influence bond strength to enamel. It is not possible to state which antimicrobial agent is better to be associated.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The effect of antimicrobial agents on bond strength of orthodontic adhesives: a meta‐analysis of in vitro studies

Altmann

Collares

Leitune

et al. 2015

Orthod Craniofacial Res

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“…The purpose of these treatments is to change the tooth surface properties to increase the bond strength [7,8]. Because surface roughness (SR) and surface wettability (SW) of biomaterials can affect biofilm formation [3,4,[9][10][11][12][13], the changes in surface properties associated with the orthodontic bonding procedure may significantly influence biofilm development around orthodontic appliances.…”

Section: Introductionmentioning

confidence: 99%

Orthodontic bonding procedures significantly influence biofilm composition

Jeon

Lim

et al. 2020

Prog Orthod.

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Background: Because changes in surface properties affect bacterial adhesion, orthodontic bonding procedures may significantly influence biofilm formation and composition around orthodontic appliances. However, most studies used a mono-species biofilm model under static conditions, which does not simulate the intraoral environment and complex interactions of oral microflora because the oral cavity is a diverse and changeable environment. In this study, a multi-species biofilm model was used under dynamic culture conditions to assess the effects of the orthodontic bonding procedure on biofilm formation and compositional changes in two main oral pathogens, Streptococcus mutans and Porphyromonas gingivalis. Methods: Four specimens were prepared with bovine incisors and bonding adhesive: untreated enamel surface (BI), enamel surface etched with 37% phosphoric acid (ET), primed enamel surface after etching (PR), and adhesive surface (AD). Surface roughness (SR), surface wettability (SW), and surface texture were evaluated. A multi-species biofilm was developed on each surface and adhesion amounts of Streptococcus mutans, Porphyromonas gingivalis, and total bacteria were analyzed at day 1 and day 4 using real-time polymerase chain reaction. After determining the differences in biofilm formation, SR, and SW between the four surfaces, relationships between bacteria levels and surface properties were analyzed. Results: The order of SR was AD < PR < BI < ET, as BI and ET showed more irregular surface texture than PR and AD. For SW, ET had the greatest value followed by PR, BI, and AD. S. mutans and P. gingivalis showed greater adhesion to BI and ET with rougher and more wettable surfaces than to AD with smoother and less wettable surfaces. The adhesion of total bacteria and S. mutans significantly increased over time, but the amount of P. gingivalis decreased. The adhesion amounts of all bacteria were positively correlated with SR and SW, irrespective of incubation time. Conclusions: Within the limitations of this study, changes in SR and SW associated with orthodontic bonding had significant effects on biofilm formation and composition of S. mutans and P. gingivalis.

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“…Acting against yeast and bacterial infections, this chemical antiseptic eliminates both gram-positive and gram-negative bacteria. In addition, it is a bacteriostatic agent, capable of inhibiting reproduction of bacteria [12][13][14] . The possibility of adding low CHX concentrations to resin materials without causing major changes to their physicochemical properties has been pointed out in previous studies 14,15 .…”

Section: Introductionmentioning

confidence: 99%

“…The possibility of adding low CHX concentrations to resin materials without causing major changes to their physicochemical properties has been pointed out in previous studies 14,15 . Antimicrobial action assessment is an important step in the development of novel restoration materials 12 .…”

Section: Introductionmentioning

confidence: 99%

In vitro evaluation of the antibacterial behavior of a self-etch adhesive associated with chlorhexidine

Rodrigues

2019

Rev. odontol. UNESP

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Introduction Adhesive technology has been developed quickly since its introduction about fifty years ago. Its goal is to produce a close contact within enamel and dentin. Failures in this process could result in microleakage, and allow the infiltration of bacteria, fluids and ions. Recent studies have been shown the use of chlorhexidine associated with adhesive systems can be able to inhibit the bacteria action. Objective The aim of this study was to evaluate the addiction of different percentages of chlorhexidine to a self-etch adhesive. Methodology The solution of digluconatechlorhexidine was increased to the primer of the two pass self etch adhesive to create a 0.5%, 1.0% and 2.0% chlorhexidine primer solution and after they were distributed in four groups (G1, G2, G3 and G4). Saliva samples (N=10) were used to test bacteria activity. Result They were spread in a blood medium with filter paper disks containing the different treatments. After the incubation, the inhibitions halos formation were evaluated. Conclusion This study demonstrated that, in vitro, the addition of different percentages of chlorhexidine digluconate to the self-etch adhesive induced inhibited halos at bacteria of saliva samples, independent from their concentration.

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In vitro study of the antibacterial properties and microbial colonization susceptibility of four self-etching adhesives used in orthodontics

Abstract: CPB has shown antimicrobial properties in vitro, and, provided the limitations of an in vitro study, the use of this self-etching adhesive may contribute to reduce microbial decalcification, making the use of this self-etching adhesive an attractive option for bracket bonding.

Cited by 12 publications

References 30 publications

The effect of antimicrobial agents on bond strength of orthodontic adhesives: a meta‐analysis of in vitro studies

The effect of antimicrobial agents on bond strength of orthodontic adhesives: a meta‐analysis of in vitro studies

Orthodontic bonding procedures significantly influence biofilm composition

In vitro evaluation of the antibacterial behavior of a self-etch adhesive associated with chlorhexidine

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