In vitro study of manganese-doped bioactive glasses for bone regeneration

Miola, Marta; Brovarone, Chiara Vitale; Maina, Giovanni; Rossi, Federica; Bergandi, Loredana; Ghigo, Dario; Saracino, Silvia; Maggiora, Marina; Canuto, Rosa Angela; Muzio, Giuliana; Verné, Enrica

doi:10.1016/j.msec.2014.01.045

Cited by 115 publications

(48 citation statements)

References 33 publications

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“…Hence, inorganic irons such as strontium [81][82][83][84][85][86], manganese [87], copper [88][89][90] have been considered to be promising agents to enhance the positive effect coatings on osteointegration and bone regeneration. Because the apatite structure is very tolerant to ionic substitutions (F, Mg, Sr, etc.…”

Section: Materials or Coatings Methodsmentioning

confidence: 99%

Review of the biocompatibility of micro-arc oxidation coated titanium alloys

et al. 2015

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Section: Materials or Coatings Methodsmentioning

confidence: 99%

Review of the biocompatibility of micro-arc oxidation coated titanium alloys

et al. 2015

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“…For these reasons, a new promising research field has been recently proposed— i.e. , the “genetic design” of new bioactive glass formulations [11] by doping silicate and phosphate glasses with several active ions, including trace elements [12,13]. …”

Section: Introductionmentioning

confidence: 99%

Bioactive and Antibacterial Glass Powders Doped with Copper by Ion-Exchange in Aqueous Solutions

Miola

Verné

2016

Materials

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In this work, two bioactive glass powders (SBA2 and SBA3) were doped with Cu by means of the ion-exchange technique in aqueous solution. SBA2 glass was subjected to the ion-exchange process by using different Cu salts (copper(II) nitrate, chloride, acetate, and sulphate) and concentrations. Structural (X-ray diffraction-XRD), morphological (Scanning Electron Microscopy-SEM), and compositional (Energy Dispersion Spectrometry-EDS) analyses evidenced the formation of crystalline phases for glasses ion-exchanged in copper(II) nitrate and chloride solutions; while the ion-exchange in copper(II) acetate solutions lead to the incorporation of higher Cu amount than the ion-exchange in copper(II) sulphate solutions. For this reason, the antibacterial test (inhibition halo towards S. aureus) was performed on SBA2 powders ion-exchanged in copper(II) acetate solutions and evidenced a limited antibacterial effect. A second glass composition (SBA3) was developed to allow a greater incorporation of Cu in the glass surface; SBA3 powders were ion-exchanged in copper(II) acetate solutions (0.01 M and 0.05 M). Cu-doped SBA3 powders showed an amorphous structure; morphological analysis evidenced a rougher surface for Cu-doped powders in comparison to the undoped glass. EDS and X-ray photoelectron spectroscopy (XPS) confirmed the Cu introduction as Cu(II) ions. Bioactivity test in simulated body fluid (SBF) showed that Cu introduction did not alter the bioactive behaviour of the glass. Finally, inhibition halo test towards S. aureus evidenced a good antimicrobial effect for glass powders ion-exchanged in copper(II) acetate solutions 0.05 M.

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“…Both, cell lines and primary cells are included (Gough, Clupper et al 2004;Huang, Jayasinghe et al 2005;Jones, Tsigkou et al 2007;Deb, Mandegaran et al 2010;Fan, Kalia et al 2014;Xynos et al, 2000;Xynos et al, 2001) BMSC Primary Bone marrow-derived mesenchymal stromal cell (BMSC), early osteoblast precursor. 2012;Miola, Brovarone et al 2014;Hoppe, Brandl et al 2015;Porwal, Estili et al 2015;Lopes, Magalhaes et al 2016;Chan, Lew et al 2018;Thavornyutikarn, Feltis et al 2019) Saos-2 Cell line "Sarcoma osteogenic cells", derived from a 11-year-old Caucasian female osteosarcoma patient (Fogh, Fogh et al 1977). (Qazi, Hafeez et al 2017;Qazi, Berkmann et al 2018;Westhauser, Karadjian et al 2019) MG-63 Cell line Osteosarcoma cells, derived from a 13-year-old male Caucasian patient (Billiau, Edy et al 1977;Czekanska, Stoddart et al 2012).…”

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confidence: 99%

Primary osteoblasts, osteoblast precursor cells or osteoblast‐like cell lines: Which human cell types are (most) suitable for characterizing 45S5‐bioactive glass?

Wilkesmann

Fellenberg

Nawaz

et al. 2019

J Biomedical Materials Res

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The question how bioactive glasses (BGs) influence the viability and osteogenic differentiation of human osteogenic cells has already been addressed by several studies.However, a literature review revealed great differences in the type of cells used for these experiments. Primary human osteoblasts (hOBs) represent the desired standard, but possess the limitation of patient variability and time-consuming isolation protocols. Therefore, several alternative cell types have been used including primary mesenchymal stromal cells (BMSCs) and the "osteoblast-like" cell lines MG-63, Saos-2, HOS, and U2OS. The aim of our study was the identification of the cell type most suitable for tissue engineering projects involving BGs by comparative analysis of cell viability and osteogenic differentiation in response to crystallized 45S5-BG. We observed that hOBs, BMSCs, and MG-63 cells were resistant to 45S5-BG induced cytotoxicity, while the viability of Saos-2, HOS, and U2OS cells was significantly reduced. In addition, we detected alkaline phosphatase activity, except in U2OS cells, that increased upon 45S5-BG cocultivation, demonstrating the induction of osteogenic differentiation. Our data and the fact that the donor-dependent variations can be avoided when using MG-63 cells suggest that these are a promising alternative to primary cells and remain an important cell line for future BG related studies.

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In vitro study of manganese-doped bioactive glasses for bone regeneration

Cited by 115 publications

References 33 publications

Review of the biocompatibility of micro-arc oxidation coated titanium alloys

Review of the biocompatibility of micro-arc oxidation coated titanium alloys

Bioactive and Antibacterial Glass Powders Doped with Copper by Ion-Exchange in Aqueous Solutions

Primary osteoblasts, osteoblast precursor cells or osteoblast‐like cell lines: Which human cell types are (most) suitable for characterizing 45S5‐bioactive glass?

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