In Vitro Study of Degradation Behavior, Cytotoxicity, and Cell Adhesion of the Atactic Polylactic Acid for Biomedical Purposes

Morozov, A. G.; Razborov, D. A.; Egiazaryan, Tatevik A.; Батенькин, М. А.; Aleynik, Diana Ya.; Egorikhina, Marfa N.; Rubtsova, Yulia P.; Charikova, Irina N.; Чесноков, С. А.; Fedushkin, Igor L.

doi:10.1007/s10924-020-01803-x

Cited by 16 publications

(15 citation statements)

References 60 publications

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“…[ 12 ] Further, we have shown that PLA obtained from the prepared lactide is biocompatible and undergoes hydrolytic decomposition in an aqueous medium in vitro. [ 13 ]…”

Section: Introductionmentioning

confidence: 99%

“…[12] Further, we have shown that PLA obtained from the prepared lactide is biocompatible and undergoes hydrolytic decomposition in an aqueous medium in vitro. [13] Infection associated with the implantation is one of the most serious problems for modern high-tech surgery. [14] Prevention of periprosthetic infection is as much important as treatment.…”

Section: Introductionmentioning

confidence: 99%

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Porous Polymer Scaffolds based on Cross‐Linked Poly‐EGDMA and PLA: Manufacture, Antibiotics Encapsulation, and In Vitro Study

Чесноков

Aleynik

Kovylin

et al. 2021

Macromolecular Bioscience

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Porous polymer materials derived from poly(ethylene glycol dimethacrylate) (poly‐EGDMA) and antibiotic containing polylactide (PLA) are obtained for the first time. Porous poly‐EGDMA monoliths with a system of open interconnected pores are synthesized by a visible light‐induced radical polymerization of EGDMA in the presence of 70 wt% of porogenic agent, e.g., 1‐butanol, 1‐hexanol, 1‐octanol, or cyclohexanol. The porosity of the obtained polymers is 75–78%. A modal pore size depends on the nature of the porogen and varies from 0.5 µm (cyclohexanol) to 12 µm (1‐butanol). The polymer matrix made with 1‐butanol features the presence of pores ranging from 1 to 100 µm. The pore surface of poly‐EGDMA matrices is inlayered with poly‐D,L‐lactide (Mn 23 × 103 Da, PDI 1.31). The PLA‐modified poly‐EGDMA retains a porous structure that is similar to the initial poly‐EGDMA but with improved strength characteristics. The presence of antibiotic containing PLA ensures a high and continuous antibacterial activity of the hybrid polymeric material for 7 days. The nontoxicity of all the porous matrices studied makes them promising for clinical tests as osteoplastic materials.

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“…[ 12 ] Further, we have shown that PLA obtained from the prepared lactide is biocompatible and undergoes hydrolytic decomposition in an aqueous medium in vitro. [ 13 ]…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Porous Polymer Scaffolds based on Cross‐Linked Poly‐EGDMA and PLA: Manufacture, Antibiotics Encapsulation, and In Vitro Study

Чесноков

Aleynik

Kovylin

et al. 2021

Macromolecular Bioscience

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“…According the Chinese standard (GB/T 16886.5-2003) materials with cell viability greater than 75% represent no cytotoxicity [64,65]. Therefore, based on the results obtained in this study, it is possible to consider that nanoparticles achieved high cell viability for both murine melanoma tumor cell lines, B16F10, and human keratinocyte cells, HaCaT., and can be considered promising for other biological studies such as in vivo tests.…”

Section: Resultsmentioning

confidence: 72%

Well Defined Poly(Methyl Methacrylate)-Fe3O4/Poly(Vinyl Pivalate) Core–Shell Superparamagnetic Nanoparticles: Design and Evaluation of In Vitro Cytotoxicity Activity Against Cancer Cells

et al. 2020

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The objective of this work is to develop and characterize polymeric nanoparticles with core–shell morphology through miniemulsion polymerization combined with seeded emulsion polymerization, aiming at the application in the treatment of vascular tumors via intravascular embolization. The synthesis of the core–shell nanocomposites was divided into two main steps: (i) Formation of the core structure, consisting of poly(methyl methacrylate)/magnetic oxide coated with oleic acid (OM-OA) via miniemulsion and (ii) shell structure produced through seeded emulsion polymerization of vinyl pivalate. Nanocomposites containing about 8 wt.% of OM-OA showed high colloidal stability, mean diameter of 216.8 nm, spherical morphology, saturation magnetization (Ms) of 4.65 emu·g−1 (57.41 emu·g−1 of Fe3O4), preserved superparamagnetic behavior and glass transition temperature (Tg) of 111.8 °C. TEM micrographs confirmed the obtaining of uniformly dispersed magnetic nanoparticles in the PMMA and that the core–shell structure was obtained by seeded emulsion with Ms of 1.35 emu·g−1 (56.25 emu·g−1 of Fe3O4) and Tg of 114.7 °C. In vitro cytotoxicity assays against murine tumor of melanoma (B16F10) and human Keratinocytes (HaCaT) cell lines were carried out showing that the core–shell magnetic polymeric materials (a core, consisting of poly(methyl methacrylate)/Fe3O4 and, a shell, formed by poly(vinyl pivalate)) presented high cell viabilities for both murine melanoma tumor cell lines, B16F10, and human keratinocyte cells, HaCaT.

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“…The second approach is characterized by examining the residual mass of the scaffold after incubation in the body-fluid-like solution. This approach is the more commonly applied of the two as it can be used for most types of scaffolds [22,23].…”

Section: Introductionmentioning

confidence: 99%

Aspects of In Vitro Biodegradation of Hybrid Fibrin–Collagen Scaffolds

et al. 2021

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The success of the regenerative process resulting from the implantation of a scaffold or a tissue-engineered structure into damaged tissues depends on a series of factors, including, crucially, the biodegradability of the implanted materials. The selection of a scaffold with appropriate biodegradation characteristics allows for synchronization of the degradation of the construct with the processes involved in new tissue formation. Thus, it is extremely important to characterize the biodegradation properties of potential scaffold materials at the stage of in vitro studies. We have analyzed the biodegradation of hybrid fibrin–collagen scaffolds in both PBS solution and in trypsin solution and this has enabled us to describe the processes of both their passive and enzymatic degradation. It was found that the specific origin of the collagen used to form part of the hybrid scaffolds could have a significant effect on the nature of the biodegradation process. It was also established, during comparative studies of acellular scaffolds and scaffolds containing stem cells, that the cells, too, make a significant contribution to changes in the biodegradation and structural properties of such scaffolds. The study results also provided evidence indicating the dependency between the pre-cultivation period for the cellular scaffolds and the speed and extent of their subsequent biodegradation. Our discussion of results includes an attempt to explain the mechanisms of the changes found. We hope that the said results will make a significant contribution to the understanding of the processes affecting the differences in the biodegradation properties of hybrid, biopolymer, and hydrogel scaffolds.

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In Vitro Study of Degradation Behavior, Cytotoxicity, and Cell Adhesion of the Atactic Polylactic Acid for Biomedical Purposes

Cited by 16 publications

References 60 publications

Porous Polymer Scaffolds based on Cross‐Linked Poly‐EGDMA and PLA: Manufacture, Antibiotics Encapsulation, and In Vitro Study

Porous Polymer Scaffolds based on Cross‐Linked Poly‐EGDMA and PLA: Manufacture, Antibiotics Encapsulation, and In Vitro Study

Well Defined Poly(Methyl Methacrylate)-Fe3O4/Poly(Vinyl Pivalate) Core–Shell Superparamagnetic Nanoparticles: Design and Evaluation of In Vitro Cytotoxicity Activity Against Cancer Cells

Aspects of In Vitro Biodegradation of Hybrid Fibrin–Collagen Scaffolds

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