In Vitro Study of Cricket Chitosan’s Potential as a Prebiotic and a Promoter of Probiotic Microorganisms to Control Pathogenic Bacteria in the Human Gut

Abstract

In this study, cricket chitosan was used as a prebiotic. Lactobacillus fermentum, Lactobacillus acidophilus, and Bifidobacterium adolescentis were identified as probiotic bacteria. Cricket chitin was deacetylated to chitosan and added to either De Man Rogosa and Sharpe or Salmonella/Shigella bacterial growth media at the rates of 1%, 5%, 10%, or 20% to obtain chitosan-supplemented media. The growth of the probiotic bacteria was monitored on chitosan-supplemented media after 6, 12, 24, and 48 h upon incubation at 37 °C. Growth of Salmonella typhi in the presence of probiotic bacteria in chitosan-supplemented media was evaluated under similar conditions to those of the growth of probiotic bacteria by measuring growth inhibition zones (in mm) around the bacterial colonies. All chitosan concentrations significantly increased the populations of probiotic bacteria and decreased the populations of pathogenic bacteria. During growth, there was a significant pH change in the media with all probiotic bacteria. Inhibition zones from probiotic bacteria growth supernatant against Salmonella typhi were most apparent at 16 mm and statistically significant in connection with a 10% chitosan concentration. This study suggests cricket-derived chitosan can function as a prebiotic, with an ability to eliminate pathogenic bacteria in the presence of probiotic bacteria.

Antimicrobial Activity of Chemically and Biologically Treated Chitosan Prepared from Black Soldier Fly (Hermetia illucens) Pupal Shell Waste

“…These results are similar to those of many previous studies [42,[58][59][60], which demonstrated higher activity of chitosan against Gram-negative than Gram-positive bacteria. This study, however, differs from some previous studies that demonstrated chitosan as having stronger activity against Gram-positive than Gramnegative bacteria [61][62][63][64]. The killing mechanism of chitosan has not yet been well studied, but several postulates have been proposed thus far.…”

Chitosan and Chitooligosaccharide: The Promising Non-Plant-Derived Prebiotics with Multiple Biological Activities

“…The consequences demonstrated that chitosan significantly increased the population of Lactobacillus spp. but decreased that of S. typhimurium [ 114 ]. The prebiotic function of chitin derivatives in inhibiting intestinal pathogenic bacteria can be largely ascribed to the inability of most pathogens to utilize them.…”

Effect of the combination of biological, chemical control and agronomic technique in integrated management pea root rot and its productivity

“…Colony diameter of the fungus in the dual culture as well as of the test fungus was recorded. Per cent growth inhibition of the pathogen over control was calculated according to the formula given by Kipkoech et al 17 as: where I = % Inhibition; C = Colony diameter in control T = Colony diameter in treatment.…”