In vitro study of adherent mandibular osteoblast-like cells on carrier materials

Turhani, Dritan; Weißenböck, Martina; Watzinger, Elisabeth; Yerit, Kaan; Cvikl, Barbara; Ewers, Rolf; Thurnher, Dietmar

doi:10.1016/j.ijom.2004.10.023

Cited by 56 publications

(48 citation statements)

References 24 publications

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“…According to Turhani et al 33 , the degree of interconnection of the pores and pore size, modulate cellular penetration, and proliferation within materials show osteoconductive properties. Pores with diameters of smaller than 10 µm inhibit cellular ingrowth, while pore sizes of between 15 and 50 µm in diameter aid in fibrovascular colonization.…”

Section: Discussionmentioning

confidence: 99%

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Porous biodegradable polyurethane nanocomposites: preparation, characterization, and biocompatibility tests

et al. 2010

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A porous biodegradable polyurethane nanocomposite based on poly(caprolactone) (PCL) and nanocomponents derived from montmorillonite (Cloisite ® 30B) was synthesized and tested to produce information regarding its potential use as a scaffold for tissue engineering. Structural and morphological characteristics of this nanocomposite were studied by infrared spectroscopy (FTIR), X-ray diffraction (XRD), small angle X-ray scattering (SAXS) and scanning electron microscopy (SEM). The reaction between polyurethane oligomers with isocyanate endcapped chains and water led to the evolution of CO 2 , which was responsible for building interconnected pores with sizes ranging from 184 to 387 µm. An in vitro cell-nanocomposite interaction study was carried out using neonatal rat calvarial osteoblasts. The ability of cells to proliferate and produce an extracellular matrix in contact with the synthesized material was assessed by an MTT assay, a collagen synthesis analysis, and the expression of alkaline phosphatase. In vivo experiments were performed by subcutaneously implanting samples in the dorsum of rats. The implants were removed after 14, 21, and 29 days, and were analyzed by SEM and optical microscopy after tissue processing. Histology crosssections and SEM analyses showed that the cells were able to penetrate into the material and to attach to many location throughout the pore structure. In vitro and in vivo tests demonstrated the feasibility for polyurethane nanocomposites to be used as artificial extracellular matrices onto which cells can attach, grow, and form new tissues.

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Section: Discussionmentioning

confidence: 99%

“…Such a biomacromolecule is essential for bone formation and is expressed from early stages of differentiation onwards 33 . The osteoblast collagen production was analyzed by a SIRCOL assay performed on the culture's supernatant.…”

Section: In Vitro Testmentioning

confidence: 99%

Porous biodegradable polyurethane nanocomposites: preparation, characterization, and biocompatibility tests

et al. 2010

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“…In previous human studies evaluating maxillary sinus augmentation after 6 months and 7 years, GBO has been demonstrated to be well integrated in the host tissues and to produce new bone with features similar to pre-existing osseous tissue (Orsini et al, 2005;Orsini et al, 2007) and in vitro is well qualified to be a substrate for cell seeding (Jensen et al, 2006;Acil et al, 2002). These results are in contrast with the data demonstrating that the proliferation rate of osteoblast-like cells seeded on GBO was lower respect to other biomaterials (Turhani et al, 2005;Wiedmann-Al-Ahmad et al, 2005;Schmitt et al, 2008). According with other authors (Wiedmann-Al-Ahmad et al, 2005) it is believed that one reason for these different results could be the source of the cells that could develop a different growth behaviour, and another reason could be the different culture conditions (e.g., seeding density, cultivation duration, medium of culture, inductive medium).…”

Section: Introductionmentioning

confidence: 72%

“…The authors conclude that Bio-Oss is "an excellent support for tissue reconstruction due to their biological characteristic and their ability to support the growth and the differentiation". However, several papers comparing Bio-Oss with other materials have already shown that Bio-Oss is a poor inducer of both cell proliferation and differentiation at least in vitro (e.g., Schmitt et al, 2008, Turhani et al, 2005. Please comment!…”

Section: Discussion With Reviewersmentioning

confidence: 99%

Functional assay, expression of growth factors and proteins modulating bone-arrangement in human osteoblasts seeded on an anorganic bovine bone biomaterial

Trubiani

Fulle²,

Traini³

et al. 2010

eCM

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The basic aspects of bone tissue engineering include chemical composition and geometry of the scaffold design, because it is very important to improve not only cell attachment and growth but especially osteodifferentiation, bone tissue formation, and vascularization. Geistlich BioOss ® (GBO) is a xenograft consisting of deproteinized, sterilized bovine bone, chemically and physically identical to the mineral phase of human bone.In this study, we investigated the growth behaviour and the ability to form focal adhesions on the substrate, using vinculin, a cytoskeletal protein, as a marker. Moreover, the expression of bone specific proteins and growth factors such as type I collagen, osteopontin, bone sialoprotein, bone morphogenetic protein-2 (BMP-2), BMP-7 and de novo synthesis of osteocalcin in normal human osteoblasts (NHOst) seeded on xenogenic GBO were evaluated. Our observations suggest that after four weeks of culture in differentiation medium, the NHOst showed a high affinity for the three dimensional biomaterial; in fact, cellular proliferation, migration and colonization were clearly evident. The osteogenic differentiation process, as demonstrated by morphological, histochemical, energy dispersive X-ray microanalysis and biochemical analysis was mostly obvious in the NHOst grown on threedimensional inorganic bovine bone biomaterial. Functional studies displayed a clear and significant response to calcitonin when the cells were differentiated. In addition, the presence of the biomaterial improved the response, suggesting that it could drive the differentiation of these cells towards a more differentiated osteogenic phenotype. These results encourage us to consider GBO an adequate biocompatible three-dimensional biomaterial, indicating its potential use for the development of tissue-engineering techniques.

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“…The main benefit of coral-derived bone void fillers over synthetic alternatives is their bioresorption capacity [25,26]. Recently, other sustainable calcified marine structures and organisms, such as mussels [27, cuttlefish bone [28] and algae [26,29,30] have been investigated for bone void filler applications.…”

Section: Tm and Biocoralmentioning

confidence: 99%

An in vitro Study to Assess the Potential of a Unique Micro porous Algal Derived Cap Bone Void Filler in Comparison with Clinically-Used Bone Void Fillers

Walsh¹

2011

J Tissue Sci Eng

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In vitro study of adherent mandibular osteoblast-like cells on carrier materials

Cited by 56 publications

References 24 publications

Porous biodegradable polyurethane nanocomposites: preparation, characterization, and biocompatibility tests

Porous biodegradable polyurethane nanocomposites: preparation, characterization, and biocompatibility tests

Functional assay, expression of growth factors and proteins modulating bone-arrangement in human osteoblasts seeded on an anorganic bovine bone biomaterial

An in vitro Study to Assess the Potential of a Unique Micro porous Algal Derived Cap Bone Void Filler in Comparison with Clinically-Used Bone Void Fillers

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