2015
DOI: 10.1515/cipms-2015-0077
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In vitro study: binding of 99mTc-DPD to synthetic amyloid fibrils

Abstract: This paper is an report of the investigation of the in vitro binding of 99mTc-DPD for synthetic amyloid fibrils used for the diagnosis of cardiac amyloidosis (CA), as compared with the use of 99mTc-HMDP and 99mTc-PPI. It also includes an inquiry into the role played by Ca2+ ions and serum proteins on binding to amyloid like materials, as well as the saturability and specificity of DPD for fibrils versus amorphous precipitates (AP). In the work, synthetic insulin fibrils (SIF) and AP were characterized by … Show more

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Cited by 6 publications
(2 citation statements)
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References 29 publications
(26 reference statements)
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“…A study showed that [ 99m Tc]Tc-HMDP uptake is significantly correlated with histological amyloid load in endomyocardial biopsy [24]. Also, an in-vitro study showed a higher affinity of [ 99m Tc]Tc-DPD for amyloid fibrils than [ 99m Tc]Tc-HMDP or [ 99m Tc]Tc-PYP [25]. Wollenweber and al. suggest that [ 99m Tc]Tc-DPD scintigraphy is useful to detect patients with a higher risk for polyneuropathy associated to ATTR-CM [26].…”
Section: Discussionmentioning
confidence: 99%
“…A study showed that [ 99m Tc]Tc-HMDP uptake is significantly correlated with histological amyloid load in endomyocardial biopsy [24]. Also, an in-vitro study showed a higher affinity of [ 99m Tc]Tc-DPD for amyloid fibrils than [ 99m Tc]Tc-HMDP or [ 99m Tc]Tc-PYP [25]. Wollenweber and al. suggest that [ 99m Tc]Tc-DPD scintigraphy is useful to detect patients with a higher risk for polyneuropathy associated to ATTR-CM [26].…”
Section: Discussionmentioning
confidence: 99%
“…However, the binding mechanism of labeled aprotinin is highly specific for fibrils and very different from that observed with 99m Tc-DPD and –PPi, which bind amorphous precipitates with the same kinetics of fibrils [ 38 ]. More recently, Thelander et al demonstrated that the binding of bone tracers to amyloid-containing hearts depends on an irregular presence of clouds of very tiny calcifications, which seem not to be directly associated with amyloid fibrils [ 39 ].…”
Section: Labeled Aprotinin In the Present Amyloid Cardiac Imaging Lan...mentioning
confidence: 99%