In-vitro Studies on the Development of Rat Peritoneal Mast Cells

Czarnetzki, Beate M.; Hannich, Dagmar; Niedorf, H. R.

doi:10.1016/s0171-2985(80)80080-5

Cited by 19 publications

(5 citation statements)

References 12 publications

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“…While studying the potential modulation of ECF release from rat peritoneal macrophages, elicited after injections with distilled water (9), in vitro culture of these cells under special conditions (see next) revealed that, within a few days of culture, the majority of the cells had assumed metachromatic staining properties that increased on more extended culture. A series of additional studies confirmed that these cultured cells exhibited indeed all major mast cell characteristics, including their mediator contents and their ability to bind immunoglobulin E (IgE; see Table 1) (10).…”

Section: Mast Cell Originmentioning

confidence: 83%

“…As SCF is also produced by diverse other cell types in the tissue, including keratinocytes and endothelial cells (reviewed in reference 31), this factor would be ideal to explain the preferential tissue accumulation of mast cells in organs like the skin or the lungs. In the fibroblast culture supernatants used for mast cell culture (10), as well as in keratinocyte supernatants, we could indeed identify SCF together with other mast cell growth factors (35,36) (see next).…”

Section: Mast Cell Growth Factorsmentioning

confidence: 84%

“…As to sources of mast cell precursors from tissues other than bone marrow, culturing of mast cells from murine spleen cells or human cord blood is routinely used by many labs. In our studies mentioned earlier, we obviously also dealt with mast cell precursors in rat peritoneal exudates (10) and in human peripheral blood (11,29), although there are only very few CD34+ cells in the latter (21). Interestingly, CD45+, c‐kit+, FcεRIα+ murine hair follicle stem cells have recently also been shown to serve as a source of mast cells (30), explaining perhaps the punctate accumulation of cutaneous mast cells in mastocytosis of the urticaria pigmentosa type and indicating that stem cells in tissue should be looked at more thoroughly as a possible source of local mast cells.…”

Section: Mast Cell Originmentioning

confidence: 99%

“…The main reason why, in our initial cultures with rat peritoneal macrophages, we were able to elicit mast cell growth, was the addition of l ‐cell fibroblast supernatants, as suggested by the macrophage experts in the experimental dermatology labs where the initial studies on mast cell differentiation was performed (10). With the discovery of SCF as a major growth, chemotactic and anti‐apoptotic factor for mast cells and other hematopoietic cells (reviewed in references 31,32), this molecule seemed a major prerequisite of mast cell development (Table 3).…”

Section: Mast Cell Growth Factorsmentioning

confidence: 99%

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Exploring the mast cell enigma: a personal reflection of what remains to be done

Henz

2008

Experimental Dermatology

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Mast cells are traditionally viewed as effector cells of allergic reactions and parasitic diseases, but their importance in host defense against bacteria, in tissue remodelling, their bone marrow and stem cell origin and a central role of the stem cell factor (SCF) as mast cell growth and chemotactic factor has been worked out only in recent years. Despite this, major aspects about the nature of the cells and their role in disease remain unclear. This holds in particular for the identification of mast cell precursors and the role of growth factors that stimulate specific mast cell commitment from stem cells, such as nerve growth factor, neutrotrophin-3 and certain interleukins, alone and during interaction with SCF. Early data suggesting also an involvement of specific transcription factors need to be expanded in this process. Furthermore, although mast cell proliferative disease (mastocytosis) has been shown to be often associated with SCF receptor c-kit mutations, reasons for the development of this disease remain unclear. This holds also for mast cell release mechanisms in many types of mast cell-dependent urticaria. Exciting new insights are emerging regarding the role of mast cells in bacterial infections, in defense against tumors, in wound healing and in the interplay with the nervous system, with hormones, and in the neurohormonal network. The aim of this reflection is to delineate the many known and unknown aspects of mast cells, with a special focus on their development, and to discuss in detail two mast cell-related diseases, namely mastocytosis and urticaria.

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Section: Mast Cell Originmentioning

confidence: 83%

Section: Mast Cell Growth Factorsmentioning

confidence: 84%

Section: Mast Cell Originmentioning

confidence: 99%

Section: Mast Cell Growth Factorsmentioning

confidence: 99%

See 2 more Smart Citations

Exploring the mast cell enigma: a personal reflection of what remains to be done

Henz

2008

Experimental Dermatology

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“…14 Mast cells of some species are more susceptible than others to hypotonic shock.12 Intraperitoneal (IP) injections of distilled water in rats causes selective disruption and death of subserous mast cells , whereas injection of Tyrode solution has little effect on these cells.15 Peritoneal washouts at 5 days after IP injection of distilled water contains predominately eosinophils , neutrophils , lymphocytes , and macrophages , but only 1 % mast cells. 16 In our in vitro study, malignant murine mast cells (P815 ) were also osmotically disrupted . Deionized water had slightly greater killing effect on malignant mast cells than distilled water ; however it was not significant .…”

Section: Discussionmentioning

confidence: 99%

Mast cell tumor destruction by deionized water

Grier,

Di Guardo,

Schaffer

et al. 1990

ajvr

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SUMMARY In a controlled study, malignant murine P815 mastocytoma cells exposed in vitro to distilled and deionized water died as a result of progressive swelling, degranulation, and membrane rupture. A 90% mean cell death occurred when cells obtained directly from culture were exposed to deionized water for 2 minutes. Of 6 cryopreserved malignant murine cell lines, which included Cloudman S91 melanoma, CMT-93 rectum carcinoma, MMT-06052 mammary carcinoma, and S-180 Sarcoma, only P815 mastocytoma and YAC-1 lymphoma were significantly (P < 0.05) affected by hypotonic shock; Cloudman S91 melanoma cells were the most resistant. Mastocytoma cells were selectively killed by hypotonic solution, and lymphoma cells were also killed by isotonic saline solution. Local mast cell tumor (mct) recurrence and percentage survival were evaluated in 12 cats (21 mct) and 54 dogs (85 mct) subjected to surgery alone or local infiltration of deionized water as an adjunct to surgery. Of all 16 incompletely excised mct in cats, there was no local recurrence following injection. Four mast cell tumors (2 cats) regressed after being injected in situ. In dogs with clinical stage-I mct, local recurrence was detected in 50% (5/10), but with injection after incomplete excision, local mct recurrence was significantly (P < 0.05) less (6.6%, 1/15). Percentage recurrence was significantly (P < 0.05) less and survival significantly greater when incompletely excised grade-II mct were injected. Mean follow-up period after surgery in cats and dogs was 35 and 23.4 months, respectively.

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Characterization of rat tissue cultured mast cells

Chan¹,

Hu³

et al. 1990

Eur J Immunol

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Twelve continuous rat tissue cultured mast cell (MC) lines were established by prolonged culture of rat peritoneal MC in the absence of added factors or feeder layers. Two of these lines, RCMC1 and RCMC2, have been briefly described previously, seven others are now also described. Both RCMC1 and RCMC2 lack a marker chromosomes present on RBL-CA10.7 cells. All lines were found to express the phenotype of mucosal MC as defined by alcian blue-positive and safranin O-negative staining, the presence of rat MC protease II and a low histamine content. When analyzed for high-(Fc epsilon RI) and low-affinity (Fc epsilon RL) receptors for IgE, the various lines yielded a variety of receptor patterns. Northern blot analysis of the RNA of RCMC1, RCMC2 and RBL-CA10.7 revealed that all three cell lines contained the same mRNA species for the alpha, beta and gamma subunits for Fc epsilon RI previously found in another rat basophilic leukemia cell line. Quantitation of the relative amounts of alpha, beta and gamma mRNA did not correlate with the expression of the relative amounts of Fc epsilon RI(alpha) in these cells. The relative amounts of mRNA for all these subunits of RCMC2 were equal or higher than those of RCMC1, suggesting that the low expression of Fc epsilon RI(alpha) on the former was a consequence of post-transcriptional events. Analysis of a RCMC1 clone over a 6-month period revealed changes in the expression of both Fc epsilon RI(alpha) and Fc epsilon RL.

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In-vitro Studies on the Development of Rat Peritoneal Mast Cells

Cited by 19 publications

References 12 publications

Exploring the mast cell enigma: a personal reflection of what remains to be done

Exploring the mast cell enigma: a personal reflection of what remains to be done

Mast cell tumor destruction by deionized water

Characterization of rat tissue cultured mast cells

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