In vitro studies on the T-lymphocyte population of human milk.

Parmely, Michael J.; Beer, Alan E.; Billingham, R. E.

doi:10.1084/jem.144.2.358

Cited by 126 publications

(51 citation statements)

References 20 publications

(25 reference statements)

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“…Several research groups reported CMC to re- (Archambault et al, 1988a,b). Background CPM are generally higher in MGL than in PBMC (Parmely et al, 1976;Concha et al, 1980;Nonnecke and Kehrli, 1985), as demonstrated in the current study. Specifically for CMC, this higher background CPM might be explained by the activating effects of colostrum on leukocytes (Sugisawa et al, 2001;Reber et al, 2006).…”

Section: Discussionsupporting

confidence: 54%

“…Specifically for CMC, this higher background CPM might be explained by the activating effects of colostrum on leukocytes (Sugisawa et al, 2001;Reber et al, 2006). Overall, MGL, both in cattle and in humans, seem not to react as strong as PBMC when confronted in vitro with mitogens and certain antigens (Parmely et al, 1976;Nonnecke and Harp, 1985;Park et al, 1993), as demonstrated in the present study for the mitogen ConA but not for the antigen TT. However, antigen-induced proliferation strongly depends on the antigen used (Smith and Goldman, 1968;Parmely et al, 1977;Archambault et al, 1988b).…”

Section: Discussionsupporting

confidence: 47%

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Effect of β-hydroxybutyric acid, parity, and body condition score on phenotype and proliferative capacity of colostral mononuclear leukocytes of high-yielding dairy cows

Meganck

Goddeeris

Campeneere³

et al. 2015

Journal of Dairy Science

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In neonatal calves, the ingestion of colostrum is imperative for preventing infectious diseases. Investigations into the transfer of passive immunity of cattle have primarily focused on the importance of colostral immunoglobulins, with a recent increase in focus on understanding the role of colostral leukocytes. The main objective of the present study was to measure the influence of parity, body condition score, serum nonesterified fatty acids, and serum β-hydroxybutyrate concentrations of periparturient cows on phenotype and mitogen-and antigen-induced proliferative capacity of bovine colostral leukocytes. Holstein-Friesian cows (n = 141) were intramuscularly vaccinated at 60 and 30 d before the expected parturition date with a tetanus toxoid vaccine. Of these 141 animals, 28 primiparous and 72 multiparous cows were sampled immediately after parturition. Colostrum mononuclear cell populations were identified by flow cytometry using bovine cluster of differentiation markers, and the proliferative capacity of these cells was determined using a 3 H-thymidine proliferation assay. Under-conditioned cows had a significantly higher percentage of colostral macrophages than normal-conditioned animals, whereas over-conditioned cows had significantly more colostral B-lymphocytes. Serum β-hydroxybutyrate was significantly associated with higher numbers of colostral T-lymphocytes and macrophages. Heifers had significantly higher mitogen-and antigen-induced proliferation of their colostral leukocytes than third parity or older cows. In conclusion, body condition score, parity, and serum β-hydroxybutyrate concentration of periparturient high-yielding dairy cows were shown to influence the number of colostral macrophages or the mitogen-and antigen-induced proliferation of colostral leukocytes, possibly influencing the cellular immunity of the newborn calf.

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Section: Discussionsupporting

confidence: 54%

Section: Discussionsupporting

confidence: 47%

Effect of β-hydroxybutyric acid, parity, and body condition score on phenotype and proliferative capacity of colostral mononuclear leukocytes of high-yielding dairy cows

Meganck

Goddeeris

Campeneere³

et al. 2015

Journal of Dairy Science

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“…Recent studies of the immunologic composition of human and animal milk have centered on the presence of selected lymphocyte populations in milk (12), migration of lymphoblasts from the gutassociated lymphoid system to the mammary gland (8,13,18,19), and the local production or concentration of immunoglobulins in the mammary gland (1 1).…”

Section: Speculationmentioning

confidence: 99%

IGE and IGD in Human Colostrum and Plasma

1982

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SummaryUsing radioimmunoassay techniques, we measured IgE and IgD levels in paired colostrum and plasma samples obtained within 4 days postpartum. In colostrum, IgE was detected in concentrations of 0.5-6 IU/ml in 16 out of 39 samples (41%) and <0.5 IU/ml in the remainder, whereas IgD was detected in all samples in concentrations of 2-2000 pg/dl. Only a moderate correlation was found between colostral and plasma levels of both IgE (r = +0.60) and IgD (r = +0.74). The correlation coefficient between IgE and IgD in plasma was 0.23, whereas in colostrum it was only 0.05. The colostrum:plasma ratio of IgE varied strikingly from that of IgD, tbe ratio of IgD was 0.1-22.2 times that of IgE. The findings argue against passive transfer of IgE and IgD from the circulation to milk and suggest possible local mammary production of either or both of these two immunoglobulins. SpeculationIn some patients, local production of IgE and/or IgD probably occurs in the mammary gland. One may hypothesize that previous antigenic exposure at other mucosal surfaces may not only determine the IgA antibody content of milk but also may influence the specificity of antibodies belonging to other immunoglobulin classes that appear in human milk. Whether the IgE and IgD antibodies in breast milk have similar specificities for antigens as the IgA antibodies in milk remains an interesting but unanswered question.Recent studies of the immunologic composition of human and animal milk have centered on the presence of selected lymphocyte populations in milk (12), migration of lymphoblasts from the gutassociated lymphoid system to the mammary gland (8,13,18,19), and the local production or concentration of immunoglobulins in the mammary gland (1 1).Local production of secretory IgA, the principal irnmunoglobulin of human milk, has been supported by the data of several investigators (5, 11). Studies comparing IgM and IgG antibodies in human milk and serum suggest either a local production or a mechanism of concentrating IgM in the mammary gland. This production or mechanism is not likely for IgG (1 1). Studies of IgE in milk have been few. Two investigators found either absent or low concentrations of IgE in milk compared with those in serum and concluded that local production or selective secretion by the mammary gland does not take place (16,17).Although IgD levels have been examined in milk (7,15), simultaneous plasma and milk levels have not been compared using a technique as sensitive as radioimmunoassay (RIA). Because of the lack of such information we used RIA to study both IgE and IgD levels in milk and peripheral blood specimens obtained simultaneously from women postpartum. The purpose of our study was to determine whether or not there is evidence to suggest either production or concentration of IgE or IgD in the mammary gland or passive transfer of either of these immunoglobulins from the circulation. By studying both immunoglobulins in paired milk and plasma samples, we obtained data suggesting the local production of one or both in at lea...

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“…the substantial number of immune cells observed in colostrum and milk (Parmely et al, 1976) produce IgD specific antibody. The present results can be critically compared with those of Keller et al (1985) who used colostrum rather than milk, plasma rather than sera and a colostrum/plasma ratio which is the reciprocal of the S/M ratio employed in the current study.…”

Section: >68mentioning

confidence: 99%

“…These cells are predominantly macrophages, but include B and T lymphocytes (Parmely, Beer & Billingham, 1976). Breast feeding has been reported to protect the human newborns against gastrointestinal, respiratory and possibly allergic problems; the mechanism is unclear and the observations remain controversial.…”

Section: Introducttonmentioning

confidence: 99%

Selective concentration of IgD class-specific antibodies in human milk

Litwin

Zehr

Insel

1990

Clinical and Experimental Immunology

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SUMMARYThe participation of human IgD class antibody in local immune responses of breast tissue was studied by analysing the sera-to-milk ratios of total IgD, IgM, IgA, IgG isotypes and albumin found in matched samples, and by analysing the sera-to-milk (S/M) ratios of IgD, IgM, IgA, IgG antibodies against Haemophilus influenzae capsular polysaccharide (PRP), phosphoryleholine, tetanus and in some cases diphtheria antigens. The study group consisted of eight women immunized during pregnancy with PRP, and control, unimmunized women. Albumin, and total IgG showed high S/M ratios. IgA had a low S/M ratio as expected, consistent with reports that IgA is locally concentrated. Total IgD and IgM isotype ratio values were intermediate between IgG and IgA suggesting they were selectively concentrated in breast fluids due to local production or transport mechanisms, or both. Ratios for specific antibodies of IgA and IgM isotypes and for total IgA and IgM isotype showed parallel data. Among the IgD antibodies, those specific for PRP and phosphoryleholine suggested a higher degree of selective concentration as compared with tetanus antigen. In the group of unimmunized women, although selective concentration of total IgD was observed, specific antibody studies were inconclusive due to the low milk IgD antibody levels encountered. The results indicate that IgD (and also IgM) may participate in local immune responses of human breast tissues and fluids; possibly influenced by the nature ofthe antigen, the state of immunization and the hormonal environment (pregnancy).

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In vitro studies on the T-lymphocyte population of human milk.

Cited by 126 publications

References 20 publications

Effect of β-hydroxybutyric acid, parity, and body condition score on phenotype and proliferative capacity of colostral mononuclear leukocytes of high-yielding dairy cows

Effect of β-hydroxybutyric acid, parity, and body condition score on phenotype and proliferative capacity of colostral mononuclear leukocytes of high-yielding dairy cows

IGE and IGD in Human Colostrum and Plasma

Selective concentration of IgD class-specific antibodies in human milk

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