In vitro studies on different extracts of fenugreek (<i>Trigonella spruneriana</i>BOISS.): Phytochemical profile, antioxidant activity, and enzyme inhibition potential

Aylanç, Volkan; Eskin, Bülent; Zengin, Gökhan; Dursun, Mehmet; Çakmak, Yavuz Selim

doi:10.1111/jfbc.13463

Cited by 16 publications

(9 citation statements)

References 56 publications

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“…The high concentration of these chemical compounds provides a large number of hydroxyl groups in the reaction medium, thus, the probability of hydrogen donation to free radicals increases, and this is reflected in the antiradical result of the tested sample (Castro‐López et al ., 2017; Carbone et al ., 2020). For example, the hydrogen atoms of adjacent hydroxyl groups (o‐diphenol) in various positions of the A, B, and C rings in quercetin, the double bonds of the benzene, and the double bond (‐C = O) of the oxo functional group ensure the compound to exhibit higher antioxidant capacity (Aylanc et al ., 2020), due to its ability for delocalisation of the radical. The findings given above confirmed as well that microwave‐assisted extraction may be a promising technique for removing antioxidant compounds from natural resources including from resistant structures like bee pollen.…”

Section: Resultsmentioning

confidence: 99%

Performance of green and conventional techniques for the optimal extraction of bioactive compounds in bee pollen

Aylanç

Ertosun

Russo‐Almeida

et al. 2022

Int J of Food Sci Tech

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The exploitation of phenolic compounds in different fields has motivated researchers to explore ecofriendly and efficient extraction techniques. This study aimed to comparatively reveal that green extraction techniques (microwave-and ultrasound-assisted) are alternative to conventional extraction (maceration and magnetic stirring) with positive impact on the phenolic content, antioxidant activity, and bioactive profile of bee pollen extracts. The highest total phenolic and flavonoid content was reached using the microwave-assisted technique (MAE) with equivalent values of 28 and 8 mg g −1 , with magnetic stirring and maceration showing a lower value. The composition profile of the extracts revealed the presence of twenty-six bioactive compounds, including thirteen phenolics and thirteen phenylamides. Although the extraction technique had little impact on the chemical diversity, the amount of bioactive compounds raised significantly with the use of the green extraction techniques, with gains between 40% and 60% for phenolics and up to 200% for phenylamides. The radical scavenging activity and the reducing power of the extracts confirmed that bee pollens are potent antioxidant source, with the most bioactive extracts corresponding to green extraction techniques. Consequently, all findings recommend the use of MAE as the technique most effective for the extraction of bioactive compounds from naturally encapsulated structures such as bee pollen.

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Section: Resultsmentioning

confidence: 99%

Performance of green and conventional techniques for the optimal extraction of bioactive compounds in bee pollen

Aylanç

Ertosun

Russo‐Almeida

et al. 2022

Int J of Food Sci Tech

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“…These compounds exhibit the potential for the treatment of various ailments e.g. diabetes (type I and type II), chronic cough, diarrhea, ulcer and rickets (Anoopkumar et al, 2020;Aylanc et al, 2020;El Sohaimy et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Microwave assisted drying and extraction technique; kinetic modelling, energy consumption and influence on antioxidant compounds of fenugreek leaves

Khan

Ghauri²,

Alvi

et al. 2022

Food Sci. Technol

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Fenugreek leaves contains bioactive compounds, which are helpful in maintaining human health. These compounds are adversely affected in conventional drying methods. Therefore, this study was carried out to retain maximum amount of these components through microwave-assisted drying and extraction technique. The technique was optimized for simultaneous drying and extraction process for fenugreek leaves. The drying rate and drying time were influenced by the level of microwave power; time was reduced from 21 to 5 minutes, when microwave power was changed from 30 to 100 W. Unlikely, the drying rate increased from 1.79 to 4.56 ± 0.05 g/(100 g.min) with the change in power (30 to 100 W). Moreover, moisture ratio analysis determined that two term model was the best to demonstrate the fitness with experimental values. The energy consumption was lowest for 100W compared to other powers. Furthermore, comparison between microwave-based extraction and methanol-extraction indicated that the antioxidant activity was better preserved compared to conventional ones even at higher microwave power levels.

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“…The current study is the first report for the estimation of the TPC and TFC of T. stellata, where their TPC was estimated as 32.8 ± 1.57 mg GAE/g and their TFC was 5.6 ± 0.14 mg RE/g. However, several previous studies discussed the contents of TP and TF in other Trigonella species, e.g., T. spruneriana [30] and T. foenum graecum leaves [31], seeds [32], and explant [33]. The phenolic compounds represent an important class of secondary metabolites with pronounced antioxidant properties.…”

Section: Resultsmentioning

confidence: 99%

“…This means that the observed antioxidant activity of T. stellata extract could be attributable to its content of phenolic constituents. As previously mentioned, the current study is the first report for the analysis of the phytochemical content and investigation of the antioxidant capability of T. stellata; nevertheless, the antioxidant potential of several Trigonella species was previously reported [30][31][32][33]. Among these reports, one study tested the antioxidant activity of T. foenum graecum using different in vitro and ex vivo methods and concluded potent activity for the seed extract [36].…”

Section: Biological Activities 221 Antioxidant Activitymentioning

confidence: 97%

Characterization of Possible α-Glucosidase Inhibitors from Trigonella stellata Extract Using LC–MS and In Silico Molecular Docking

Elwekeel

Amir

Mohamed

et al. 2022

Plants

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The current study accentuates the significance of performing the multiplex approach of LC-HRESIMS, biological activity, and docking studies in drug discovery, taking into consideration a review of the literature. In this regard, the investigation of antioxidant and cytotoxic activities of Trigonella stellata collected from the Egyptian desert revealed a significant antioxidant capacity using DPPH with IC50 = 656.9 µg/mL and a moderate cytotoxicity against HepG2, MCF7, and CACO2, with IC50 values of 53.3, 48.3, and 55.8 µg/mL, respectively. The evaluation of total phenolic and flavonoid contents resulted in 32.8 mg GAE/g calculated as gallic acid equivalent and 5.6 mg RE/g calculated as rutin equivalent, respectively. Chemical profiling of T. stellata extract, using LC-HRESIMS analysis, revealed the presence of 15 metabolites, among which eleven compounds were detected for the first time in this species. Interestingly, in vitro testing of the antidiabetic activity of the alcoholic extract noted an α-glucosidase enzyme inhibitory activity (IC50 = 559.4 µg/mL) better than that of the standard Acarbose (IC50 = 799.9 µg/mL), in addition to a moderate inhibition of the α-amylase enzyme (IC50 = 0.77 µg/mL) compared to Acarbose (IC50 = 0.21 µg/mL). α-Glucosidase inhibition was also virtualized by binding interactions through the molecular docking study, presenting a high binding activity of six flavonoid glycosides, as well as the diterpenoid compound graecumoside A and the alkaloid fenugreekine. Taken together, the conglomeration of LC-HRESIMS, antidiabetic activity, and molecular docking studies shed light on T. stellata as a promising antidiabetic herb.

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In vitro studies on different extracts of fenugreek (Trigonella sprunerianaBOISS.): Phytochemical profile, antioxidant activity, and enzyme inhibition potential

Cited by 16 publications

References 56 publications

Performance of green and conventional techniques for the optimal extraction of bioactive compounds in bee pollen

Performance of green and conventional techniques for the optimal extraction of bioactive compounds in bee pollen

Microwave assisted drying and extraction technique; kinetic modelling, energy consumption and influence on antioxidant compounds of fenugreek leaves

Characterization of Possible α-Glucosidase Inhibitors from Trigonella stellata Extract Using LC–MS and In Silico Molecular Docking

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