In vitro Studies on Antidiabetic Potential of New Dosage Forms of AYUSH 82

Abstract

AYUSH 82 powder is an Ayurvedic antidiabetic formulation developed by the Central Council for Research in Ayurvedic Sciences (CCRAS), Ministry of AYUSH, Government of India. It comprises ingredients traditionally used for their beneficial effect in diabetes (Prameha/Madhumeha). The hypoglycemic effects of AYUSH 82 powder have been reported in diabetic subjects. In the current study, the antidiabetic potential of AYUSH 82 powder along with its two new dosage forms -AYUSH 82 mixture extract and AYUSH 82 compound extractwas investigated in vitro for elucidating mechanism of their action by possible α-amylase inhibitory property, insulindependent glucose uptake in skeletal muscle cell line (C2C12 myotubes), and effect on peroxisome proliferator-activated receptor gamma (PPAR-γ) activity.All the three dosage forms of AYUSH 82 -powder, mixture extract, and compound extract -exhibited inhibition of α-amylase activity. AYUSH 82 mixture extract, however, demonstrated highest extent of inhibition in both methanolic (87.4%) and aqueous (48.2%) format. All the three dosage forms of AYUSH 82 also demonstrated an increase in insulin-dependent glucose uptake in C2C12 myotubes as compared with control. However, none of the test items (TIs) exhibited activation of PPAR-γ expression in tested ranges, indicating that antidiabetic potential of TIs may not be mediated via PPAR-γ activation. Results indicated that the new dosage forms of AYUSH 82 (mixture extract and compound extract) may be useful for making new dosage forms of AYUSH 82 as tablets/capsule, etc.

Evaluation of Antidiabetic Potential of New Dosage Forms of AYUSH 82 in Streptozotocin-induced Type II Diabetic Rats

“…5 The antidiabetic potential of AYUSH 82 powder along with its two new dosage forms-mixture extract and compound extract when investigated in vitro through mechanism of their action exhibited inhibition of α-amylase activity and an increase in insulin-dependent glucose uptake in C2C12 myotubes as compared with control. 6 In the current study, we investigated the in vivo antidiabetic potential of AYUSH 82 mixture in comparison to AYUSH 82 mixture extract with Shilajit. The study was conducted in streptozotocin (STZ)-induced type II diabetic rat model.…”