In vitro studies of the antiherpetic effect of photodynamic therapy

Abstract

The number of viral infection cases in the Department of Gynecology and Obstetrics has tended to increase over last few years. Viruses form herpesvirus and cytomegalovirus families are associated with an increased risk for recurrent pregnancy loss. Photodynamic therapy (PDT) is a promising new approach to treat viral infections in which viral particles are inactivated. It exhibits great therapeutic potential, particularly among this group of patients. This study examined the use of PDT to treat herpesvirus infection (HVI) using an in vitro model. In this study, we used the Vero сell lineage as a suitable model of HVI, strains of HSV-1 (strain VR-3) and HSV-2 (strain MS) obtained from The National Virus Collection (London, UK), the photosensitizer Fotoditazine (Veta-Grand, Russia), an AFS physiotherapeutic device (Polironic Corporation, Russia). Laser light irradiation and the photosensitizer had different cytotoxic effects on the Vero cell cultures depending on the doses used. The optimal laser light and photosensitizer doses were determined. PDT had an antiviral effect on an in vitro model of HVI in cell culture. PDT has been shown to be effective treatment for HVI in vitro, leading to a reliable decrease of viral titer.

Trends and targets in antiviral phototherapy

“…Photoditazine, a water-soluble glucosamine derivative of chlorin e 6 (27), has been used in an in vitro study against HSV to obtain a 1.5-2.5 log reduction of the virus titer. 283 The same compound had earlier been shown to be effective in a cervical cancer model associated with HPV 16, a HPV type known to have a high probability of leading to neoplastic lesions. 284 Another chlorin derivative, 3-phorbinepropanol-9,14-diethyl-4,8,13,18-tetramethyl-20-(3S-trans) (28) was tested on the bovine viral diarrhea virus (BVDV, enveloped virus) and the encephalomyocarditis virus (EMCV, non-enveloped) in the context of blood sterilization.…”

Educação Em Saúde Sobre Diabetes Mellitus Para Componentes Do Programa De Atenção À Terceira Idade (Proati): Relato De Experiência

“…Ademais, a TFD demonstrou potencial para tratar infecções virais, como hepatite C, herpes simplex e síndrome da imunodeficiência adquirida, além de ser sugerida para o tratamento de herpes labial, bem como infecções, lesões e cânceres associados a vírus (LIM et al, 2012;LOTUFO et al, 2020;ZVEREV et al, 2016). E, na descontaminação de produtos infectados por micro-organismos e sangue, a tecnologia fotodinâmica é usada principalmente na desinfecção de instrumentos 66 (SILVA et al, 2019).…”

Photodynamic Therapy for Oral Herpes Simplex Infections: It can be Possible?

“…Some articles on the treatment of HSV infections are present in scientific literature. In vitro studies made by Zverev et al [6] shown high sensibility of HSV-1 and Type 2 spp to LLLT mediated by Fotoditazine cromophore leading to a reliable decrease of viral agents content [6].…”