In vitro Studies of Iron Chelation Activity of Purified Active Ingredients Extracted from Triticum aestivum Linn. (Wheat Grass)

Abstract

Objective: Seven to eight days germinated plants of Triticum aestivum (wheat grass) are a rich source of vitamin A, C, calcium, magnesium, phosphorus, potassium, sodium, sulphur, cobalt, zinc and protein. Traditionally the aqueous extract of T. aestivum was reported to be used as a health tonic in folk and ayurvedic medicine. We previously reported that aqueous extract of T. aestivum was found to reduce the blood transfusion requirement in iron overloaded Thalassemia and Myelodysplastic syndrome patients. Our objective was to extract and purify active ingredients from wheat grass and study their mode of action in stabilizing hemoglobin level in those patients. Design and Method: Active ingredients of wheat grass were extracted and purified by cation exchange column chromatography followed by High Performance Liquid Chromatography. In vitro experiments with phenylhydrazine treated red blood cell hemolysate were carried out before and after treatment of purified fraction of T. aestivum to study iron chelating activity. Result: Purified fraction of T. aestivum treated red blood cell showed significant inhibition

The Comparison of the Chemical Composition, Sensory, Phenolic and Antioxidant Properties of Juices from Different Wheatgrass and Turfgrass Species

“…A very few publications are available in literature on nutritive and antioxidant properties of wheatgrass juice where it is reported that these extracts contain high levels of antioxidants and inhibit the DNA oxidative damage and are effective in suppressing the superoxide radical (Kulkarni et al, 2006;Rana, 2011). It has also shown antimutagenicity (Alitheen et al, 2011;Arya and Kumar, 2011), antimicrobial (Ashok, 2011;Pallavi et al, 2011;Das et al, 2012), antiallergic (Padalia et al, 2010) and diuretic (Popovič et al, 2014) properties. Wheatgrass was also reported to be helpful incuring certain diseases such as thalassemia (Singh et al, 2010), distal ulcerative colitis, anaemia, diabetes, eczema, constipation, and ulcerative…”

Chelators in Iron and Copper Toxicity

“…Curcuminoids have been shown to bind in vitro and alleviate toxicity in thalassemic mice [31,32]. T h e i r o n c h e l a t i n g a c t i v i t y o f A f r i c a n w a l n u t (Tetracarpidium conophorum) and wheat grass (Triticum aestivum) extracts have been studied and the results suggest this may have potential for iron overload in thalassaemia patients [33][34][35]. The African walnut contains a number of phenolic compounds which are believed to chelate to iron or copper [34].…”

“…T h e i r o n c h e l a t i n g a c t i v i t y o f A f r i c a n w a l n u t (Tetracarpidium conophorum) and wheat grass (Triticum aestivum) extracts have been studied and the results suggest this may have potential for iron overload in thalassaemia patients [33][34][35]. The African walnut contains a number of phenolic compounds which are believed to chelate to iron or copper [34]. The active chelators in wheat grass appear to be derivatives of mugineic acid, 3-hydroxy-mugineic acid and 2′deoxy-mugineic acid [35].…”

Development of Wheatgrass-Pomegranate Blended Therapeutical Juice Using Response Surface Methodology

“…It is also known as green blood because it enhances the production of red blood cells (Grunewald 2009). It lowers the blood pressure (Locniskar 1988), revitalizes energy (Kulkarni et al 2006), produces the required efficacy against diabetes (Samagandi et al 2012), purifies the body, aids weight loss, possesses antioxidant property (Kulkarni et al 2006;Siener et al 2006;, fights against free radicals (Borek 2002;Kulkarni et al 2006), detoxifies (Das et al 2012) and promotes healthy skin.…”