In vitro Studies of Functionalized Mesoporous Silica Nanoparticles for Photodynamic Therapy

Tu, Hsiung‐Lin; Lin, Yu-Shen; Lin, Hsia Yu; Hung, Yu-Chueh; Lo, Leu‐Wei; Chen, Yang‐Fang; Mou, Chung‐Yuan

doi:10.1002/adma.200800548

Cited by 197 publications

(101 citation statements)

References 31 publications

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“…16 Therefore, these materials have been proposed for use as potential vehicles for biomedical imaging, real-time diagnosis, and controlled delivery of multiple therapeutic agents. [6][7][8]10,[17][18][19][20][21][22][23][24][25] Despite the considerable interest in the biomedical applications of MSNs, relatively few studies have been published on the biocompatibility of the two most common types of MSNs (MCM-41 and SBA-15). [26][27][28][29] Asefa and co-workers reported that the cellular bioenergetics (cellular respiration and ATP levels) were inhibited remarkably by large SBA-15 nanoparticles, but the inhibition was greatly reduced by smaller MCM-41-type nanoparticles.…”

Section: Introductionmentioning

confidence: 99%

Interaction of Mesoporous Silica Nanoparticles with Human Red Blood Cell Membranes: Size and Surface Effects

Zhao¹,

Sun²,

Zhang³

et al. 2011

ACS Nano

493

436

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The interactions of mesoporous silica nanoparticles (MSNs) of different particle sizes and surface properties with human red blood cell (RBC) membranes were investigated by membrane filtration, flow cytometry, and various microscopic techniques. Small MCM-41-type MSNs (∼100 nm) were found to adsorb to the surface of RBCs without disturbing the membrane or morphology. In contrast, adsorption of large SBA-15-type MSNs (∼600 nm) to RBCs induced a strong local membrane deformation leading to spiculation of RBCs, internalization of the particles, and eventual hemolysis. In addition, the relationship between the degree of MSN surface functionalization and the degree of its interaction with RBC, as well as the effect of RBC−MSN interaction on cellular deformability, were investigated. The results presented here provide a better understanding of the mechanisms of RBC−MSN interaction and the hemolytic activity of MSNs and will assist in the rational design of hemocompatible MSNs for intravenous drug delivery and in vivo imaging. R ecent advancements in particle size and morphology control of mesoporous materials have led to the creation of nano-and submicrometer-sized mesoporous silica nanoparticles (MSNs). [1][2][3][4][5] The MSN materials with well-ordered cylindrical pore structures, such as MCM-41 and SBA-15, have attracted special interest in the biomedical field. 1 The large surface areas and pore volumes of these materials allow the efficient adsorption of a wide range of molecules, including small drugs, 6-10 therapeutic proteins, 11-13 antibiotics, 14,15 and antibodies. 16 Therefore, these materials have been proposed for use as potential vehicles for biomedical imaging, real-time diagnosis, and controlled delivery of multiple therapeutic agents. [6][7][8]10,[17][18][19][20][21][22][23][24][25] Despite the considerable interest in the biomedical applications of MSNs, relatively few studies have been published on the biocompatibility of the two most common types of MSNs (MCM-41 and SBA-15). [26][27][28][29] Asefa and co-workers reported that the cellular bioenergetics (cellular respiration and ATP levels) were inhibited remarkably by large SBA-15 nanoparticles, but the inhibition was greatly reduced by smaller MCM-41-type nanoparticles. 26 These differences in the disruption of cellular bioenergetics are believed to be caused by the different surface areas, number of surface silanol groups, and/or particle sizes of both types of material. A recent study by Kohane and collaborators on the systemic effects of MCM-41 (particle size ∼150 nm) and SBA-15 (particle size ∼800 nm) MSNs in live animals revealed interesting findings regarding their biocompatibility. 27 While large doses of mesoporous silicas administered subcutaneously to mice appear to be relatively harmless, the same doses given intravenously or intraperitoneally were lethal. 27 A possible reason for the severe systemic toxicity of MSNs when injected intravenously could be the interactions of the nanoparticles with blood cells.Our initial studies...

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Section: Introductionmentioning

confidence: 99%

Interaction of Mesoporous Silica Nanoparticles with Human Red Blood Cell Membranes: Size and Surface Effects

Zhao¹,

Sun²,

Zhang³

et al. 2011

ACS Nano

493

436

View full text Add to dashboard Cite

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“…7) with MSN through covalent bonding to yield PpIX-modified MSN (Fig. 25) for PDT studies (Tu et al 2009). …”

Section: Covalent Encapsulation Of Ps In Silica Nanoparticlesmentioning

confidence: 98%

Nanoparticles for Photodynamic Therapy Applications

Vanderesse

Frochot

Barberi‐Heyob

et al. 2011

Intracellular Delivery

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Photodynamic therapy has emerged as an alternative to chemo-and radiotherapy for the treatment of certain types of cancer. Nanoparticles have been used to improve the delivery and the efficiency of the photosensitizer as they allow its encapsulation without loss of activity. Using targeting strategies, they can also allow the selective accumulation of the photosensitizer in the cancer tissues. In this review, based on the chemical nature of the nanoparticles, the different methods for their syntheses are described from the pioneering works to the latest achievements. Indeed the nanosystems can be conjugated to a biomolecule or an antibody to target receptors over-expressed in cancer cells and/or angiogenic vascular endothelial cells. Numerous in vivo and in vitro applications have been described. Multifunctional nanoplatforms combining several applications within the same nano-object emerge as potential important theranostic tools.

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“…Firstly, singlet oxygen generated by SNG was monitored by a chemical method involving the photo-oxidation of 9,10-anthracenediyl-bis(methylene)dimalonic acid (ABDA) (Fig. S7) [39]. Then, MTT assays were employed to investigate the effect of light irradiation (2.0 mW/cm 2 , 400-700 nm) with different durations on the cytotoxicity toward HepG2 and HeLa cells in vitro.…”

Section: In Vitro Anti-tumor Efficacymentioning

confidence: 99%

pH-responsive metallo-supramolecular nanogel for synergistic chemo-photodynamic therapy

Yao

Chen

et al. 2015

Acta Biomaterialia

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In vitro Studies of Functionalized Mesoporous Silica Nanoparticles for Photodynamic Therapy

Cited by 197 publications

References 31 publications

Interaction of Mesoporous Silica Nanoparticles with Human Red Blood Cell Membranes: Size and Surface Effects

Interaction of Mesoporous Silica Nanoparticles with Human Red Blood Cell Membranes: Size and Surface Effects

Nanoparticles for Photodynamic Therapy Applications

pH-responsive metallo-supramolecular nanogel for synergistic chemo-photodynamic therapy

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