In vitro STORAGE OF SYNTHETIC SEEDS OF Aechmea fasciata

New approaches for reducing the cost of the synthetic seeds storage using sugarcane bagasse and different additives to the gel matrix for sugarcane plant in vitro.

“…2. This data was supported by previous studies using different sodium alginate levels for capsulation of cucumber (Tabassum et al, 2010), on Acacia (Nor Asmah et al, 2012 andBadr-Elden, 2013) on strawberry. Development of the isodiametric compact bead of Stevia rebaudiana was formed by using 100 mM CaCl 2 , after15 min (Ali et al, 2012).…”

“…It was noticed that, increasing storage time of synthetic seeds from 2 to 10 weeks gradually increased the regrowth of synthetic seeds. Badr-Elden (2013) showed that the storage medium supplemented with CCC, at all concentrations, the encapsulated buds of Achemia fasciata were still without regrowth after 2, 4 or 6 weeks. The growth of capsules of Stevia rebaudiana Bertoni dependent on mineral concentration and time of storage (Nower, 2014).…”

“…Grzegorczyk et al (2011) showed that regeneration of shoot and root from encapsulated shoot tips of Salvia officinalis L. after 6 weeks from germination of synthetic seeds when gel matrix contained 2% sodium alginate, GA3 (0.5 or 1 mg/L) and sucrose 15 g/L. Badr-Elden (2013) showed that encapsulated buds of Achemia fasciata lost the high amount of moisture and gives the lowest germination rate (30.63%) when add 60.0 g/L sucrose in the alginate matrix and storage for eight weeks. Increased in conversion of capsules of sugar beet and shoots turned green, produced at low concentration (30 g/L) of sucrose (Rizkalla et al, 2012).…”