In vitro Sterilization, Rooting and Acclimatization of Difficult-to-root Bougainvillea Cultivars

Kumari, Pratiksha; Swaroop, Kishan; Janakiram, T.; Singh, Shweta; Prasad, K.; Jain, Ritu; landscaping, Iari; Bhawan, New Delhi Icar Krishi Anusandhan

doi:10.23910/ijbsm/2016.7.3.1530

Cited by 3 publications

(1 citation statement)

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“…Recent investigations have shifted toward understanding the tissue culture variations among different Bougainvillea varieties, aiming to develop a more efficient system that lowers costs and enhances benefits. For instance, Kumari et al [22] conducted in vitro root culture and domestication studies on challengingto-root Bougainvillea varieties, Mahatma Gandhi and Refulgens. Jain et al [23] provided a protocol for the in vitro multiplication of the Bougainvillea cultivar Mahara.…”

Section: Introductionmentioning

confidence: 99%

An Efficient Method for the Propagation of Bougainvillea glabra ‘New River’ (Nyctaginaceae) from In Vitro Stem Segments

Lin,

Xu,

et al. 2024

Forests

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Bougainvillea, an evergreen climbing shrub of the Nyctaginaceae family, holds significant ornamental, economic, and medicinal value. Bougainvillea glabra ‘New River’ is widely used in landscapes due to its strong adaptability to the environment, abundance of flowers, and frequent flowering. Traditionally, Bougainvillea glabra ‘New River’ cultivation has relied on methods such as cuttings or grafting, with limited research on in vitro tissue culture propagation. This study aimed to optimize the tissue culture system, exploring a combination of plant growth regulators (PGRs) for Bougainvillea regeneration from in vitro stem segments. The Murashige and Skoog (MS) medium supplemented with indole-3-butyric acid (IBA), 6-benzylaminopurine (6-BA), and 1-naphthlcetic acid (NAA) was employed. The optimal sterilization of Bougainvillea stem segments involved a 30 s treatment with 75% alcohol and 10 min with 1% NaClO. The synergistic effect of 0.1 mg·L−1 of NAA and 2.5 mg·L−1 of 6-BA maximized the shoot sprouting frequency, while 2.5 mg·L−1 of 6-BA and 0.1 mg·L−1 of NAA produced the maximum shoots. Furthermore, 1.5 mg·L−1 of IBA and 0.1 mg·L−1 of NAA induced the highest rooting levels. This work demonstrates the successful adaptation of a greenhouse environment to efficiently regenerate plants in vitro from stem segments. This approach allows for the mass production of Bougainvillea glabra ‘New River’.

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Section: Introductionmentioning

confidence: 99%

An Efficient Method for the Propagation of Bougainvillea glabra ‘New River’ (Nyctaginaceae) from In Vitro Stem Segments

Lin,

Xu,

et al. 2024

Forests

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Morphological, Anatomical, Physiological and Biochemical Changes during Adventitious Roots Formation of Bougainvillea buttiana ‘Miss Manila’

et al. 2022

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Bougainvillea, a widely used ornamental plant, is mainly propagated by cuttings and tissue culture. Still, large-scale production of Bougainvillea is often difficult because of rooting issues. Therefore, based on an early establishment of the regeneration system for tissue culture in Bougainvillea by our research team, we further studied its rooting mechanism. It was observed that the morphology and anatomical structure of Bougainvillea buttiana ‘Miss Manila’ contained endogenous hormones, such as indole-3-acetic acid (IAA), abscisic acid (ABA), gibberellic acid (GA3), and zeatin-riboside (ZR), including peroxidase (POD), polyphenol oxidase (PPO), and IAA-oxidase (IAAO) activities. Following the culture, Days 0–15 consisted of the induction and initiation stages, while Days 15–25 included the expression stages. No latent root primordium was found in the Bougainvillea plantlet, which belonged to the induced rooting type. The root primordium was derived from callus cells generated by divisions of parenchyma cells in the basic tissues. It was found that the changes in the POD, PPO, and IAAO activities were closely related to the formation of adventitious roots (AR), in which the highest rooting values occurred during the transition from the initiation stage to the expression stage, whereas the endogenous IAA and ABA contents had negative and positive correlations during the induction, initiation, and expression stages.; The values of GA3 and ZR also peaked during the transition from the initiation to the expression stage. ZR and GA3 were found to promote adventitious root formation, while ABA inhibited it. The IAA/ABA, ABA/ GA3, and IAA/ZR ratios also shifted at the onset of the expression stage of AR, indicating these values were closely related to their occurrence. Overall, this study provides the basis for further research considering AR formation in Bougainvillea, and the propagation of various Bougainvillea varieties.

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Establishing an Efficient Regeneration System for Tissue Culture in Bougainvillea buttiana ‘Miss Manila’

Huang

Zhang

Zhao

et al. 2022

Plants

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Bougainvillea plants have important ornamental and cultural value, as well as applications, for example, in improving the ecological environment, sterilization and as anti-virals in medicines, etc. Among many varieties, Bougainvillea buttiana ‘Miss Manila’ is more popular in landscape applications in southern China because of its excellent qualities. However, because of the difficulty of sexual reproduction, low rooting rate of asexual propagation cuttings and low temperature tolerance, its large-scale development is limited. For easy, quick and mass multiplication of such cultivars, tissue culture technique can be put to use. In this study, nodal segments of ‘Miss Manila’ were used as explants, and a single-factor experiment was carried out with a variety of plant growth regulators and concentrations to establish and optimize a complete tissue culture propagation system. The results showed that the best disinfestation was 75% ethanol treatment for 0.5 min + 0.1% HgCl2 treatment for 9 min, and the survival rate was 86.83%. The best shoot initiation formula was MS + 6-BA (2.5 mg/L) + IBA (0.2 mg/L), and the induction rate was 95.14%. The best formula for shoot proliferation was MS + 6-BA (1.5 mg/L) + NAA (0.1 mg/L), and the proliferation coefficient was 3.13. The best rooting culture formula was 1/2 MS + IBA (2.0 mg/L), and the rooting rate was 81.97%. The survival rate for plantlet refining and transplanting was 93.33%. In this study, a relatively efficient regeneration system for the tissue culture and rapid propagation of Bougainvillea buttiana ‘Miss Manila’ was established to address the problems of rooting and transplanting of this variety, to benefit research on the industrialized production and regeneration systems of this variety, and to provide a reference for the tissue culture of other varieties of Bougainvillea or other woody plants.

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In vitro Sterilization, Rooting and Acclimatization of Difficult-to-root Bougainvillea Cultivars

Cited by 3 publications

References 0 publications

An Efficient Method for the Propagation of Bougainvillea glabra ‘New River’ (Nyctaginaceae) from In Vitro Stem Segments

An Efficient Method for the Propagation of Bougainvillea glabra ‘New River’ (Nyctaginaceae) from In Vitro Stem Segments

Morphological, Anatomical, Physiological and Biochemical Changes during Adventitious Roots Formation of Bougainvillea buttiana ‘Miss Manila’

Establishing an Efficient Regeneration System for Tissue Culture in Bougainvillea buttiana ‘Miss Manila’

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