In vitro sporulation of race 32 of Puccinia graminis Pers. f.sp. tritici Erikss. &amp; Henn

Kück, Karl‐Heinz; Reisener, H. J.

doi:10.1016/0048-4059(85)90039-6

Cited by 6 publications

(2 citation statements)

References 18 publications

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“…We have used uredospores of Pucdnia graminis and studied their germination on a water surface. Although under these conditions only hyphal growth can be observed Early Changes in the Nucleotide Pools of Germinating Rust Uredospores 3 without the development of infection structures (KucK and REISENER 1985), we were primarily interested in the very early metabolic changes of the germination process.…”

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confidence: 99%

Early Changes in the Nucleotide Pools of Germinating Rust Uredospores

Grabo

Wagner

1991

Journal of Phytopathology

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Puccinia graminis uredospores were induced to germinate on a water surface and the nucleotide/nucleoside pools were determined by high performance liquid chromatography. Distinct phases of germination could be discriminated by correlating the changes of individual nucleotide pools with characteristic morphological changes. During the first 15 min no visible changes of the cell wall were observed whereas the adenine nucleotide pools increased strongly indicating an activation of the energy metabolism. The stage preceding hyphal growth revealed large increases in the precursor pool for cell wall synthesis (UDP‐N‐acetylglucosamine). The early phases of germination are obviously controlled by distinct metabolic transitions. Furthermore, correlations of pool contents and changes during germination with the germination efficiency of the three uredospore batches investigated are discussed.

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confidence: 99%

Early Changes in the Nucleotide Pools of Germinating Rust Uredospores

Grabo

Wagner

1991

Journal of Phytopathology

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“…Depuis 1951, date à laquelle la culture artificielle in vitro de Gymnosporangium juniperi-virginianae a pu être réalisée (HO T SON & Cur'rER, 1951), de nombreux parasites obligatoires dont Puccinia graminis f. sp. trilici (WILLIAMS et al, 1966(WILLIAMS et al, et 1967GRAMBOW & RIEDEL, 1977 ;KUCK, 1979 ;KUCK & REISENER, 1985) et Melampsora lini (B OSE & S HAW , 1974) peuvent désormais donner lieu sur milieu gélosé à un développement mycélien accompagné ou non de sporulation.…”

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Les oïdiums des cucurbitacées. II. Mise au point d'une technique de conservation des souches en culture axénique

Molot¹,

Leroux²,

Ferrière³

1987

Agronomie

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La formation de cals et même de racines (cas de la courgette et du concombre) assure à la feuille une survie d'environ 2 mois (4 mois si elle n'est pas contaminée par l'oïdium). Après isolement d'une souche donnée, son repiquage « in vitro », à l'aide d'un pinceau, d'une feuille infectée à une feuille saine ne pose aucune difficulté.Cette technique est comparée aux diverses méthodes utilisées pour la conservation des parasites obligatoires.Mots clés additionnels : Sphaerotheca fuliginea, Erysiphe cichoracearum, Leveillula taurica, formation de cals, rhizogenèse, culture de tissus, courgette, concombre, tabac, piment, HgCl 2 , technique de désinfection. SUMMARYPowdery mildews of cucurbits. II. A method for preserving isolates in axenic culture.The following powdery mildews were preserved actively on detached adult leaves of different plants in a culture medium, in axenic conditions : Sphaerotheca fuliginea on squash or cucumber ; Erysiphe cichoracearum on squash, cucumber or tobacco ; Leveillula taurica on pepper. Detached leaves were first disinfected by soaking for 3 minutes in an aqueous solution of 0.05 % HgC' 2 , and their petioles planted into an agar medium (tissue culture). Leaves were then sprayed with spores of different powdery mildews, disinfected by an original technique involving HgC' 2 for less than 2 min. Spores were readily transferred from diseased to healthy leaves with a soft paint brush in axenic conditions. Callus and root formation (with cucumber and squash) allowed the leaves to survive as long as 2 months, or 4 months if not infected with powdery mildews. This technique is compared with other methods of conservation already in use for obligate parasites.

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The Uredinales

Mendgen

1997

Plant Relationships Part B

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In vitro sporulation of race 32 of Puccinia graminis Pers. f.sp. tritici Erikss. & Henn

Cited by 6 publications

References 18 publications

Early Changes in the Nucleotide Pools of Germinating Rust Uredospores

Early Changes in the Nucleotide Pools of Germinating Rust Uredospores

Les oïdiums des cucurbitacées. II. Mise au point d'une technique de conservation des souches en culture axénique

The Uredinales

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