In vitro sorption and desorption of basic fibroblast growth factor from biodegradable hydrogels

Therapeutic lymphangiogenesis using stem cell and VEGF-C hydrogel

“…[15,16]. The acidic gelatin powder (viscosity 32.0 mps; isoelectric point, 5.5), prepared from calfskin using an alkaline process, was supplied by Sammi Gelatin Co., Korea.…”

“…Tabata et al demonstrated that an acidic gelatin hydrogel can release biologically active bFGF in vitro [15]. We have developed a VEGF-C gelatin hydrogel based on polyion complexation that is composed of acidic gelatin with an isoelectric point (IEP) of 5.0, which interacts ionically with the basic VEGF-C (IEP, 8.3) [16].…”

Controlled Delivery of Basic Fibroblast Growth Factor Promotes Human Cardiosphere-Derived Cell Engraftment to Enhance Cardiac Repair for Chronic Myocardial Infarction

“…Biodegradable gelatin is a useful delivery modality to circumvent the short half-life of recombinant bFGF in vivo. We have designed a controlled-release system for bFGF composed of acidic gelatin, which forms a poly-ion complex with bFGF (8). Biodegradable hydrogels display excellent biocompatibility demonstrated by the absence of rejection and inflammation and achieve a controlled release of bFGF in vivo as a result of hydrogel degradation within 3 weeks (9).…”

Biological and biophysical principles in extracorporal bone tissue engineering

“…The mitogenic effects of FGF-1 have been associated with chondrocyte proliferation, while FGF-2 is expressed by osteoblasts in which it is generally more potent than FGF-1. Crosslinked gelatin hydrogels with b-FGF incorporated via electrostatic interaction 79 implanted into rabbit cranial defects enhanced bone regeneration as compared to free b-FGF of the same dose without carrier 80 . Various other cytokines or growth factors have also been used successfully in tissue engineering approaches for the enhancement and acceleration of craniofacial bone formation 1,10,11,39 .…”