In vitro somatic embryogenesis from cell suspension cultures of cowpea [Vigna unguiculata (L.) Walp]

Ramakrishnan, K.; Gnanam, R.; Sivakumar, P.; Manickam, A.

doi:10.1007/s00299-005-0965-5

Cited by 35 publications

(33 citation statements)

References 48 publications

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“…To improve embryogenesis in eucalyptus, glutamine and casein have been employed (Pinto et al, 2002), while in Miscanthus and Cherries cultures, 1-proline is studied to boost up early stages of somatic embryogenesis (Holme et al, 1997;Cheong and Pooler, 2004). The accumulation of amino acids during somatic embryogenesis was also reported for alfalfa, Anise (Andarwulan and Shetty, 1999), cowpea (Ramakrishnan et al, 2005) and for the shoot regeneration of strawberry (Qin et al, 2005). Moreover, Zhang et al (2006) reported that the transcription levels persuaded by TDZ were observed to vary all over TDZ treatment.…”

Section: Amino Acidsmentioning

confidence: 99%

Thidiazuron: A multi-dimensional plant growth regulator

Guo¹,

Abbasi²,

Zeb³

et al. 2011

Afr. J. Biotechnol.

195

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Thidiazuron (TDZ) has gained a considerable attention during past decades due to its efficient role in plant cell and tissue culture. Wide array of physiological responses were observed in response to TDZapplication in different plant species. TDZ has shown both auxin and cytokinin like effects, although, chemically, it is totally different from commonly used auxins and cytokinins. A number of biological (physiological and biochemical) events in cells are induced or enhanced by TDZ, but the mode of action of TDZ is yet unknown. However, varieties of underlying mechanisms were revealed by reports showing how morphogenic events were induced by application of TDZ. Other reports showed that TDZ may modify endogenous plant growth regulators, either directly or indirectly and produce reactions in cell/tissue, necessary for its division/regeneration. Other possibilities include modification in cell membrane, energy levels, nutrient absorption, transport and assimilation, etc. In this review, recent advancements in TDZ application in plant sciences are discussed.

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Section: Amino Acidsmentioning

confidence: 99%

Thidiazuron: A multi-dimensional plant growth regulator

Guo¹,

Abbasi²,

Zeb³

et al. 2011

Afr. J. Biotechnol.

195

View full text Add to dashboard Cite

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“…Previous studies suggest use of auxins at initial stage like 2,4,5-trichlorophenoxyacetic acid, (Muthukumar et al, 1995); 2,4-D (Ganapathi and Anand, 1998;Prem Anand et al, 2000 andRamakrishnan et al, 2005) followed by culturing on cytokinin-auxin combinations for induction of callus and somatic embryogenesis in cowpea. Pulse treatment with cytokinins for shoot induction in cowpea using BAP at higher dose has also been reported by Brar et al (1999) and Aasim et al (2009a).…”

Section: Introductionmentioning

confidence: 99%

In Vitro Shoot Regeneration of NAA-Pulse Treated Plumular Leaf Explants of Cowpea

Aasım

2010

Not Sci Biol

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Cowpea (Vigna unguiculata L.) is an economically important grain legume crop and is an important source of dietary protein in many of the developing countries. The present study reports the effect of pulse treatment duration, concentration of NAA and presence of NAA in the culture medium on shoot regeneration from plumular leaf explant of Turkish cowpea cv. ' Akkiz' and 'Karagoz' . Pulse treatment of mature embryos with 20 mg l -1 NAA for 1 and 3 weeks followed by culturing of plumular leaf explant on MS medium containing 0.25, 0.50 and 1.0 BAP with 1.0, 2.0 and 4.0 mg l -1 NAA promoted somatic embryogenesis in both cultivars. Longer duration of pulse treatment was deleterious resulting in browning and consequently death of the embryos on explants. Pulse treatment with 20 mg l -1 NAA for one week was less deleterious and developed two plantlets after the explants were transferred to MS0 medium after 6 weeks through somatic embryogenesis in cv. ' Akkiz' . Pulse treatment with 10 mg l -1 NAA for 1 week showed 33.33-50.00 % and 25.00-50.00% shoot regeneration frequency in cv. ' Akkiz' and 'Karagoz' respectively on MS medium containing 0.25-1.00 mg l -1 BAP. Maximum number of 2.50 shoots each per explant were recorded in cv. ' Akkiz' and 'Karagoz' on MS medium containing 1.00 and 0.50 mg l -1 BAP respectively. Contrarily, maximum shoot length of 8.98 cm of cv. ' Akkiz' and 9.42 cm of cv. 'Karagoz' was recorded on MS medium containing 0.50 mg l -1 BAP and 1.00 mg l -1 BAP respectively. Regenerated shoots were rooted on MS medium containing 0.5 mg l -1 IBA and and acclimatized in growth room at room temprature where they produced viable seeds.

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“…Cowpea, like most legumes, is recalcitrant to in vitro regeneration, which limits its potential for improvement through genetic manipulations (Ramakrishnan et al, 2005). Furthermore, variability is involved in almost every aspect of the regeneration systems explored, such as optimal explant tissues, basal salt compositions, plant growth regulators, sucrose levels, and variation in composition of gelling agents (agar or Gelrite) (Pellegrineschi, 1997;Popelka et al, 2006;Aasim et al, 2009a).…”

Section: Introductionmentioning

confidence: 99%

Production of herbicide-resistant cowpea (Vigna unguiculata L.) transformed with the bar gene

Aasım¹,

Khawar²,

Özcan³

2013

Turk J Biol

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Plant genetic engineering has opened new avenues to modify crops and has provided a powerful tool for crop improvement.\ud The present study reports the development of regeneration and genetic transformation protocol for the Turkish cowpea cultivar Akkiz.\ud The immature cotyledons were cultured on Murashige and Skoog (MS) medium containing 0.25–0.75 mg L–1 6-benzylaminopurine with\ud or without 0.25 mg L–1 α-naphthalene acetic acid. Shoot regeneration varied 44.4%–83.3% with 2.1–5.0 shoots per explant. Regenerated\ud shoots were rooted in MS medium containing 0.50 mg L–1 indole-3-butyric acid and acclimatized in the greenhouse, where they flowered\ud and set seeds. Immature cotyledons were transformed with Agrobacterium tumefaciens strain LBA4404 harboring the recombinant\ud binary vector pRGG containing an herbicide tolerance gene (bar) along with a uida (GUS) gene under 35S promoter. Phosphinothricin\ud was used as a selectable marker at a concentration of 2.5 mg L–1. Putative transformants were screened by the histochemical GUS assay.\ud Furthermore, molecular analysis revealed the presence of the introduced gene in the genome of cowpea cultivar Akkiz. The selected\ud transgenic plants showed a resistance to Basta® nonselective herbicide at up to 10 mL L–1 of water. Putative transgenic plants retained\ud their pigmentation and continued to grow in the greenhouse

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In vitro somatic embryogenesis from cell suspension cultures of cowpea [Vigna unguiculata (L.) Walp]

Cited by 35 publications

References 48 publications

Thidiazuron: A multi-dimensional plant growth regulator

Thidiazuron: A multi-dimensional plant growth regulator

In Vitro Shoot Regeneration of NAA-Pulse Treated Plumular Leaf Explants of Cowpea

Production of herbicide-resistant cowpea (Vigna unguiculata L.) transformed with the bar gene

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