In vitro shoots from root explant, their encapsulation, storage, plant recovery and genetic fidelity assessment of Limonium hybrid ‘Misty Blue’: a florist plant

Regeneration of plantlets through somatic embryogenesis from root derived calli of Hibiscus sabdariffa L. (Roselle) and assessment of genetic stability by flow cytometry and ISSR analysis

“…In the last few years, the totipotency of root explants has been established in different plants, as in Arabidopsis [ 32 ], Brassica oleracea [ 33 ] Coriandrum sativum [ 34 ], Limonium Misty Blue [ 28 ] etc. Practically the plant parts which are used as explants contain a variety of tissues that are ready to express pluripotency or totipotency but they are often inhibited by the neighboring tissues.…”

“…A factorial experiment with varying concentrations of MgCl 2 (1.5, 2.0 and 2.5 mM) and Taq DNA polymerase (0.5, 0.75 and 1 U) was performed in different PCR cycles to optimize the best PCR conditions and to obtain the best amplification results. For initial screening, a total of 25 ISSR primers were selected either from UBC series or from the previously published literatures [ 24 – 28 ]. Out of these twenty five primers, 15 primers (14–18 mer) both 5', 3' anchored and unanchored were finally selected based on their ability of production of reproducible and scorable loci.…”

“…All of the experiments were carried out in a randomized block design and each set of experiment comprised of ten explants or cultures in triplicate. The results were analyzed as mean ± standard errors (SE) and the percentage data were arcsine-transformed following the method of our previous study [ 28 ], [ 29 ]. The significant difference ( p ≤ 0.05) among the mean was assessed by analysis of variance followed by Duncan’s multiple range test (DMRT) using MSTAT-C software (ver.…”

Application of bioreactor technology in plant propagation and secondary metabolite production

“…Since one of the primary physiological problems in liquid-cultured plants is the malformation of the shoots, caused by hyperhydricity, the induction of meristematic or bud clusters with arrested leaf growth could help to overcome this problem (Ziv, 1991;Ziv and Shemesh, 1996). The choice of regeneration program that does not allow induction of variations is essential for ensuring the genetic stability of in vitro plants in conservation and micropropagation programs (Bose et al, 2017). Further studies are needed to optimize culture conditions for the specific genotypes.…”

“…Further studies are needed to optimize culture conditions for the specific genotypes. Clonal fidelity of micropropagated plants has to be monitored by their morphological, biochemical, physiological and genetic characteristics using molecular markers as powerful tools for genetic identification (Ziv, 1991;Debnath, 2011;Bose et al, 2017).…”