2017
DOI: 10.1007/s11240-017-1179-6
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In vitro shoots from root explant, their encapsulation, storage, plant recovery and genetic fidelity assessment of Limonium hybrid ‘Misty Blue’: a florist plant

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Cited by 14 publications
(10 citation statements)
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“…In the last few years, the totipotency of root explants has been established in different plants, as in Arabidopsis [ 32 ], Brassica oleracea [ 33 ] Coriandrum sativum [ 34 ], Limonium Misty Blue [ 28 ] etc. Practically the plant parts which are used as explants contain a variety of tissues that are ready to express pluripotency or totipotency but they are often inhibited by the neighboring tissues.…”
Section: Resultsmentioning
confidence: 99%
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“…In the last few years, the totipotency of root explants has been established in different plants, as in Arabidopsis [ 32 ], Brassica oleracea [ 33 ] Coriandrum sativum [ 34 ], Limonium Misty Blue [ 28 ] etc. Practically the plant parts which are used as explants contain a variety of tissues that are ready to express pluripotency or totipotency but they are often inhibited by the neighboring tissues.…”
Section: Resultsmentioning
confidence: 99%
“…A factorial experiment with varying concentrations of MgCl 2 (1.5, 2.0 and 2.5 mM) and Taq DNA polymerase (0.5, 0.75 and 1 U) was performed in different PCR cycles to optimize the best PCR conditions and to obtain the best amplification results. For initial screening, a total of 25 ISSR primers were selected either from UBC series or from the previously published literatures [ 24 28 ]. Out of these twenty five primers, 15 primers (14–18 mer) both 5', 3' anchored and unanchored were finally selected based on their ability of production of reproducible and scorable loci.…”
Section: Methodsmentioning
confidence: 99%
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“…Since one of the primary physiological problems in liquid-cultured plants is the malformation of the shoots, caused by hyperhydricity, the induction of meristematic or bud clusters with arrested leaf growth could help to overcome this problem (Ziv, 1991;Ziv and Shemesh, 1996). The choice of regeneration program that does not allow induction of variations is essential for ensuring the genetic stability of in vitro plants in conservation and micropropagation programs (Bose et al, 2017). Further studies are needed to optimize culture conditions for the specific genotypes.…”
Section: Genetic Stabilitymentioning
confidence: 99%
“…Further studies are needed to optimize culture conditions for the specific genotypes. Clonal fidelity of micropropagated plants has to be monitored by their morphological, biochemical, physiological and genetic characteristics using molecular markers as powerful tools for genetic identification (Ziv, 1991;Debnath, 2011;Bose et al, 2017).…”
Section: Genetic Stabilitymentioning
confidence: 99%