In vitro shoot multiplication of apple: comparative response of three rootstocks to cytokinines and auxin

Dobránszky, J.; Abdul-Kader, A.; Magyar-Tábori, K.; Jámbor-Benczúr, E.; Bubán, T.; Szalai, J.; Lazányi, János

doi:10.31421/ijhs/6/1/63

Cited by 6 publications

(7 citation statements)

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“…1C), confirming observations mentioned previously on seasonal variations of browning of explants from adult plants. Season of explant collection has been reported to affect the browning of apple shoot tip explants (Dobránszki et al, 2000;Modgil et al, 1999;Wang et al, 1994) as well as the survival of mango explants because of lower phenolic content (Thomas and Ravindra, 1997). Similar to the present work, shoot tip explants of 'Koroneiki' olive trees exhibited higher browning rates and total phenol and polyphenoloxidase activity compared with nodal explants, whereas explants derived from glasshouse-growing trees had higher survival rates and lower browning rates and total phenol concentrations than the corresponding explants from field-growing trees Pontikis, 2001a, 2001b).…”

Section: Resultsmentioning

confidence: 99%

“…Shoot tip explants of 'Koroneiki' olive trees exhibited higher browning rates together with higher total phenol and polyphenoloxidase activity compared with single-node explants, whereas explants derived from glasshouse-growing trees presented higher survival rates and lower total phenol and polyphenoloxidase activity than corresponding explants from field-growing trees Pontikis, 2001a, 2001b). The season of collection has been reported to affect browning of explants (Dobránszki et al, 2000;Modgil et al, 1999;Wang et al, 1994) as well as their survival through effects on the concentration of phenolics (Thomas and Ravindra, 1997). In addition, the season of explant collection has been found to affect the survival of explants through effects on microbial contamination (Thomas and Ravindra, 1997).…”

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confidence: 99%

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Season and Explant Origin Affect Phenolic Content, Browning of Explants, and Micropropagation of ×Malosorbus florentina (Zucc.) Browicz

Martini¹,

Papafotiou²,

Vemmos³

2013

horts

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The aim of this study was to develop an efficient protocol for in vitro propagation of the rare and endangered ×Malosorbus florentina, not only enabling conservation of the species, but also its use as an ornamental. Explants excised from adult plants, shoot tip explants, and explants collected in March and April showed more browning and had higher content of total phenolics than explants excised from juvenile tissue, nodal explants, and those collected during any of the other months of the year. Shoot tip explants from adult plants were more difficult to establish in vitro (14%) compared with explants from micropropagated plantlets or sprouts of burned plants (29% to 36%). Nodal explants excised from seedlings were established at the highest percentage (83%), giving the most shoots per explant (5.2). Generally, in vitro cultures established from adult plants, with the exception of one culture, showed lower multiplication rates compared with cultures from juvenile plants. Nodal explants from the base of sprouts produced a higher percentage (60%) of shoots than explants from upper locations (20% to 31%), but any differences in proliferation rates of established cultures ceased after the third subculture. Microshoots from juvenile cultures were more capable of rooting (51% to 58%) than were those from adult plants (16% to 32%), whereas 83% of the plantlets were acclimatized ex vitro independently of their origin, but plantlets of juvenile origin, although developing the same height as those originating from adult plants, had shorter internodes and thus more compact shape.

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Section: Resultsmentioning

confidence: 99%

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confidence: 99%

Season and Explant Origin Affect Phenolic Content, Browning of Explants, and Micropropagation of ×Malosorbus florentina (Zucc.) Browicz

Martini¹,

Papafotiou²,

Vemmos³

2013

horts

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“…Early studies were often hampered by tissue browning caused by oxidation of polyphenolics due to wounding during in vitro culture establishment (Dobránszki and Teixeira da Silva 2010). Reducing tissue browning is possible by timing the collection of explants, adding antioxidants to the culture medium such as ascorbic acid, activated charcoal (AC), or polyvinylpyrrolidine (PVP), singly or in combination, or the use of liquid culture or micrografting (Yepes and Aldwinckle 1994;Modgil et al 1999;Dobránszki et al 2000;Kaushal et al 2005). Mert and Soylu (2010) found that timing of in vitro culture initiation and the use of axillary or terminal shoot tips affected medium browning during shoot regeneration, with a broad range of 10-84% browning across three rootstock cultivars, M9, MM106 and MM111.…”

Section: In Vitro Propagation Of Applementioning

confidence: 99%

In vitro tissue culture of apple and other Malus species: recent advances and applications

Silva

Gulyás

Magyar-Tábori

et al. 2019

Planta

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Main conclusion Studies on the tissue culture of apple have allowed for molecular, biotechnological and applied breeding research to advance. In the past 8 years, over 100 papers advancing basic biology, genetic transformation and cryobiology have emerged. Apple (Malus × domestica Borkh.; Rosaceae) is an important fruit crop grown mainly in temperate regions of the world. In vitro tissue culture is a biotechnological technique that has been used to genetically improve cultivars (scions) and rootstocks. This updated review presents a synthesis of findings related to the tissue culture of apple and other Malus spp. between 2010 and 2018. Increasingly complex molecular studies that are examining the apple genome, for example, in a bid to identify the cause of epigenetic mutations and the role of transposable elements in this process would benefit from genetically stable source material, which can be produced in vitro. Several notable or curious in vitro culture methods have been reported to improve shoot regeneration and induce the production of tetraploids in apple cultivars and rootstocks. Existing studies have revealed the molecular mechanism underlying the inhibition of adventitious roots by cytokinin. The use of the plant growth correction factor allows hypothetical shoot production from leaf-derived thin cell layers relative to conventional leaf explants to be determined. This updated review will allow novices and established researchers to advance apple and Malus biotechnology and breeding programs.

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“…However, the results of proliferation rates in the most recent experiments were lower than in previous experiments, irrespective of whether BA or BAR was used as cytokinin. One reason for the differences in the number of new shoots could be that the auxin levels were different: in the present study the media contained 0.1 mg 1 1 instead of 0.3 mg 1 1 IBA and the auxin level could modify the multiplication rate depending on the genotype (Dobránszki et al, 2000a).…”

Section: Resultsmentioning

confidence: 69%

“…In vitro cultures of cv. Jonagold scions were established as reported earlier (Dobránszki et al, 2000a). Shoots were multiplied on MS medium (Murashige and Skoog, 1962) containing 100 mg 1 1 myo-inositol, 0.7% agar-agar and 3% saccharose, supplemented with 1.0 mg 1"' benzyladenine riboside, 0.3 mg 1 1 indole-3-butyric acid (IBA) and 0.2 mg I 1 gibberellic acid (GA 3 ) before the shoot multiplication experiments.…”

Section: Methodsmentioning

confidence: 99%

The multifunctionality of grasslands in rural development in a European context

Nagy¹

2002

AAgr

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Experiments involving the intercropping of faba bean with each of lupin, fenugreek and Egyptian clover as well as growing faba bean alone were carried out at Assiut University on a farm naturally infested with Orobanche in two seasons. The major objective of the study was to investigate the effect of different intercropping combinations with faba bean on the infestation with Orobanche.The results obtained revealed that intercropping faba bean with each of lupin, fenugreek and Egyptian clover markedly reduced the Orobanche crenata Forsk. infestation of faba bean. The number of branches, the height of the first pod, the number of pods, the seed yield and the number and dry weight of Orobanche spikes were significantly affected by the intercropping treatments, but these had no significant influence on plant height, straw yield or 100-seed weight. Intercropping faba bean with each of lupin, fenugreek and Egyptian clover increased the faba bean seed yield, consequently the economic return was also increased.

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In vitro shoot multiplication of apple: comparative response of three rootstocks to cytokinines and auxin

Cited by 6 publications

References 0 publications

Season and Explant Origin Affect Phenolic Content, Browning of Explants, and Micropropagation of ×Malosorbus florentina (Zucc.) Browicz

Season and Explant Origin Affect Phenolic Content, Browning of Explants, and Micropropagation of ×Malosorbus florentina (Zucc.) Browicz

In vitro tissue culture of apple and other Malus species: recent advances and applications

The multifunctionality of grasslands in rural development in a European context

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