In vitro Shoot Bud Differentiation from Leaf Segments of Achras sapota

Purohit, S. D.; Singhvi, A.; Nagori, R.

doi:10.1023/b:biop.0000024284.84740.75

Cited by 9 publications

(6 citation statements)

References 10 publications

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“…Direct shoot organogenesis from parenchymatic cells has been observed in Lycopersicon esculentum (Pugliesi et al 1999), Fagus orientalis (Cuenca and Vieitez 1999) and Achras sapota (Purohit et al 2004). The pattern of shoot development, via the formation of meristematic domes, was similar to those of Alstroemeria (Lin et al 1998), F. orientalis (Cuenca and Vieitez 1999), Jatropha integerrima (Sujatha et al 2000), L. esculentum (Pugliesi et al 1999), and A. sapota (Purohit et al 2004). In the last two species, as well as in chestnut (Castanea sativa Mill.…”

Section: Discussionmentioning

confidence: 99%

Biochemical and histological changes associated with in vitro responses in sunflower cotyledonary explants

Vega

Nestares

Pratta

et al. 2007

In Vitro Cell.Dev.Biol.-Plant

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In vitro shoot regeneration from sunflower cotyledonary explants can be obtained in the presence of kinetin and indole-3-acetic acid. In contrast, callus proliferation is obtained in the presence of 2,4-dichlorophenoxyacetic acid on culture medium. The purpose of this study was to investigate changes in protein profiles during callus and shoot development from cotyledonary explants and to correlate them with ontogenic stages during in vitro culture. Cotyledons cultured in the presence of 2,4-dichlorophenoxyacetic acid produced friable callus as a result of early division of parenchymatic cells associated with the vascular bundles of the explant. The callogenic ability was independent of the cotyledonary region used as starting explant. Direct shoot organogenesis was observed from the same type of cells growing in culture media supplemented with kinetin and indole-3-acetic acid. In this case, the regeneration potential varied among regions from which the explants were obtained. Protein profiles revealed differences associated with shoots or callus developmental programs. A 27-kDa polypeptide was uniquely detected in the explants undergoing shoot organogenesis. The amount of this polypeptide during the first 4 d of culture increased and was followed by the appearance of meristematic centers in histologically analyzed samples. This polypeptide could be used as a specific marker for in vitro shoot development in this species.

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Section: Discussionmentioning

confidence: 99%

Biochemical and histological changes associated with in vitro responses in sunflower cotyledonary explants

Vega

Nestares

Pratta

et al. 2007

In Vitro Cell.Dev.Biol.-Plant

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“…The air layering is very easy and quicker method of propagation but the plants propagated by this technique are shallow rooted and prone to uprooting due to high wind velocity. Multiplication of sapota by micropropagation has been tried but without much success (Bapat and Narayanaswami, 1977;Purohit et al 2004).…”

Section: Sapota Is Propagated By Various Vegetative Means As Itmentioning

confidence: 99%

“…Purohit and Singhvi (1998) developed protocol for sapota micropropagation using cotyledonary node segments and were able to induce multiple shoots from nodal segments by forced axillary branching and rooting in shoots and successfully transferred the plants in soil. Later on Purohit et al (2004) used leaf segments for shoot bud differentiation in sapota. They reported high shoot bud regeneration in explants from middle portion of leaves taken from middle part of shoots.…”

Section: Micropropagation In Sapotamentioning

confidence: 99%

Propagation Studies in Sapodilla [Manilkara zapota (L.) P. Royen]: A Review

Kaur

Boora

Singh

2020

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Sapota [Manilkara zapota (L) P. Royen] is an important commercial fruit crop of Sapotaceae family grown for fresh fruits and chicle production. Sapota when propagated by vegetative means, produce true to type plant material and reduces juvenile period and result in early commercial production. Various propagation techniques on different rootstocks were tried for the purpose. Among different rootstocks, khirni (Manilkara hexandra) was found most successful and is used for commercial plant propagation as it possesses high graft compatibility with sapota and plants produce fruits of excellent quality with high yield. Various vegetative propagation techniques have been standardized and used on large scale depending upon season and region. The general techniques used for sapota propagation are softwood grafting, veneer grafting, side grafting, inarching and air layering. Softwood grafting is employed in regions with moderate temperature and high relative humidity which prevent desiccation of scion stick. Veneer and side grafting using mature scion stick is also found effective in regions with variable climates. Inarching technique possess high graft survival rate and is practiced by private nurseries. Propagation through cuttings is not successful in sapota even when treated with rooting hormones. Micro-propagation in sapota by tissue culture techniques have been tried but application remains limited to academics only. This may be due to high phenolic contents and sap exudation in explant. Special protocols for successful root and shoot regeneration are required to be standardized for commercial scale plant propagation.

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“…The observations showed the leaf explants response with respect to hormone and its combinations for shoot buds proliferation, multiplication, shoot elongation, rooting and hardening process. Plant regeneration from leaf explant was reported in Achras sapota (Purohit et al, 2004), E. axillare (Jeyachandran et al, 2005) and Prunus serotina (Liu and Pijut, 2008). By using this method, multiple shoots have been induced directly from leaf explant which produced maximum number of shoots on MS medium containing KN, BAP and TDZ alone or in combination with NAA.…”

Section: Shoot Proliferation and Multiplicationmentioning

confidence: 99%

“…In recent years there has been an increased interest in in vitro culture techniques which offer a viable tool for mass multiplication and germplasm conservation of rare, endangered and threatened medicinal plants (Tiwari et al, 2000). There are reports of adventitious shoot regeneration through direct organogenesis using leaf explants of medicinally important species like Pothomorphe umbellata (Pereira et al, 2000), Tagetes erecta, Echinacea purpurea (Koroch et al, 2002) and Achras supota (Purohit et al, 2004). Direct plant regeneration from leaf explant of Enicostemma axillare was obtained using different concentrations of BAP with stable concentration of KN (Jeyachandran et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Adventitious shoot regeneration from leaf explants of the valuable medicinal herb Plectranthus barbatus Andrews

Thangavel¹,

Britto²,

Senthilkumar³

2013

Afr. J. Biotechnol.

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The objective of this study was to develop an efficient protocol for adventitious shoot regeneration for Plectranthus barbatus Andrews using leaf explants. The explants were cultured on MS (Murashige and Skoog, 1962) medium containing various concentration of kinetin (KN), 6-benzylaminopurine (BAP) and thidiazuron (TDZ) (0.5 to 3.0 mg/l) or in combination with α-naphthalene acetic acid (NAA) for shoot development. The highest adventitious shoot regeneration percentage (80%) and average maximum number of 19.7 ± 2.08 shoots per explants was observed on MS medium supplemented with 1.5 mg/l KN followed by 2.0 mg/l BAP with 1.0 mg/l NAA (15.0 ± 2.20 shoots per explants). The shoots were excised and the residual explants were transferred to fresh medium where again they developed shoots. The shoots were elongated when they were cultured on half strength MS medium supplemented with 0.6 mg/l gibberellic acid (GA 3) and 0.5 mg/l KN. The in vitro developed shoots produced roots when transferred to half strength MS medium augmented with 1.5 mg/l indole-3-butyric acid (IBA). The in vitro raised plantlets were successfully transferred to mixture of river sand, garden soil and saw dust (1:1:1) and was hardened in controlled environment. Hardened plantlets were transferred to soil in greenhouse.

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In vitro Shoot Bud Differentiation from Leaf Segments of Achras sapota

Cited by 9 publications

References 10 publications

Biochemical and histological changes associated with in vitro responses in sunflower cotyledonary explants

Biochemical and histological changes associated with in vitro responses in sunflower cotyledonary explants

Propagation Studies in Sapodilla [Manilkara zapota (L.) P. Royen]: A Review

Adventitious shoot regeneration from leaf explants of the valuable medicinal herb Plectranthus barbatus Andrews

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