In vitro serial subculture to improve rooting of Eucalyptus urophylla

Mendonça, Evânia Galvão; Batista, Tânia Regina; Stein, Vanessa Cristina; Balieiro, Flávia Pereira; Abreu, José Renato de; Pires, Marinês Ferreira; Souza, Patrícia Aparecida de; Paiva, Luciano Vilela

doi:10.1007/s11056-019-09761-6

Cited by 10 publications

(16 citation statements)

References 30 publications

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“…This factor becomes crucial to assess the genetical stability of in vitro-cultured plants, making their valorization easier. Moreover, long-term subcultures constitute an efficient strategy to improve interesting phenomena under in vitro conditions, such as rooting (Mendonça et al, 2019;Wang and Yao, 2020), plant regeneration (Konar et al, 2019) and callus induction (Nakasha et al, 2016), very useful for biotechnological production of by-products from medicinal plants.…”

Section: Discussionmentioning

confidence: 99%

Machine Learning Unmasked Nutritional Imbalances on the Medicinal Plant Bryophyllum sp. Cultured in vitro

et al. 2020

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Plant nutrition is a crucial factor that is usually underestimated when designing plant in vitro culture protocols of unexploited plants. As a complex multifactorial process, the study of nutritional imbalances requires the use of time-consuming experimental designs and appropriate statistical and multiple regression analysis for the determination of critical parameters, whose results may be difficult to interpret when the number of variables is large. The use of machine learning (ML) supposes a cutting-edge approach to investigate multifactorial processes, with the aim of detecting non-linear relationships and critical factors affecting a determined response and their concealed interactions. Thus, in this work we applied artificial neural networks coupled to fuzzy logic, known as neurofuzzy logic, to determine the critical factors affecting the mineral nutrition of medicinal plants belonging to Bryophyllum subgenus cultured in vitro. The application of neurofuzzy logic algorithms facilitate the interpretation of the results, as the technology is able to generate useful and understandable “IF-THEN” rules, that provide information about the factor(s) involved in a certain response. In this sense, ammonium, sulfate, molybdenum, copper and sodium were the most important nutrients that explain the variation in the in vitro culture establishment of the medicinal plants in a species-dependent manner. Thus, our results indicate that Bryophyllum spp. display a fine-tuning regulation of mineral nutrition, that was reported for the first time under in vitro conditions. Overall, neurofuzzy model was able to predict and identify masked interactions among such factors, providing a source of knowledge (helpful information) from the experimental data (non-informative per se), in order to make the exploitation and valorization of medicinal plants with high phytochemical potential easier.

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Section: Discussionmentioning

confidence: 99%

Machine Learning Unmasked Nutritional Imbalances on the Medicinal Plant Bryophyllum sp. Cultured in vitro

et al. 2020

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“…Low rooting potential in a given plant species, as reported here for the cambuci tree, is often associated with the occurrence of sclerenchyma tissues located externally to the root meristem development region (Hartmann et al, 2018;Mendonça et al, 2019).…”

Section: Anatomical Analysesmentioning

confidence: 74%

“…A similar occurrence of compounds has been observed in other members of the Myrtaceae family, such as guava and uvaia (Eugenia pyriformis Cambess. ), grumixama (E. brasiliensis), and eucalyptus (Eucalyptus urophylla; Duarte & Paula, 2005;Donato & Morretes, 2007;Gomes et al, 2009;Mendonça et al, 2019).…”

Section: Anatomical Analysesmentioning

confidence: 99%

“…It is widely known that rooting potential may be related to stem anatomy (Wendling, Brooks, & Trueman, 2015). It has been found that the anatomical structure of primary phloem combined with the degree of tissue sclerification contribute to the lack of development of adventitious roots in cuttings sampled from woody species (Mendonça et al, 2019). Therefore, elucidating the anatomy of plant tissues is key to understanding the process of rhizogenesis (Bortolini et al, 2008) as well as overcoming any difficulties that might arise in the development of adventitious roots (Lima et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

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Adventitious rooting and anatomical aspects of Campomanesia phaea stems

et al. 2022

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Current literature is lacking regarding the vegetative propagation of the cambuci tree (Campomanesia phaea O. Berg Landrum). This study aimed to verify the efficiency of cutting techniques via the assessment of cuttings of varying types and sizes, sampling dates, and the influence of treatments containing plant growth regulator indolebutyric acid (IBA) combined with the antioxidant ascorbic acid across different immersion times. The species was found not to develop adventitious roots easily, and hence, the herbaceous and woody stem materials were subjected to histochemical studies to elucidate their anatomical and physiological processes. To this end, plant material was polymerized in glycol methacrylate resin, cut into sections, and stained using toluidine blue (0.05%) or via histochemical staining with PAS/naphthol blue black. Anatomical analysis of plant structures revealed the presence of phenolic compounds and sclerenchyma tissue, which in turn are expected to negatively impact the development of adventitious roots.

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“…Serial propagation by cuttings is a promising technique for reversing adult tissue in different species of Eucalyptus (Wendling and Xavier 2005). Mendonça et al (2020) reported that in vitro rejuvenation/ reinvigoration was a determining factor for greater rooting efficiency of mini cuttings for clonal rescue in Eucalyptus urophylla S.T. Blake.…”

Section: Adventitious Rooting Of Mini Cuttingsmentioning

confidence: 99%

Evaluation of total protein, peroxidase, and nutrients measured by pXRF for the determination of tissue rejuvenation/reinvigoration of Eucalyptus microcorys

et al. 2023

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Ontogenetic aging of tissues and the gradual decrease of adventitious rooting are known challenges for the clonal propagation of woody species, hampering clonal forestry programs. This study examined possible signatures of tissue rejuvenation/reinvigoration in different propagated materials of Eucalyptus microcorys by analyzing the total protein profile, peroxidase activity, macro- and micronutrient contents, and adventitious rooting of mini cuttings. The analyses were performed on E. microcorys shoots which were successfully obtained by seminal and grafting propagation, micropropagation with epicormic shoots, and indirect organogenesis. Among four mature trees used in the propagation, tissues from the one with the best propagation results were investigated for signs of tissue rejuvenation and/or reinvigoration. Five individuals from each technique were randomly selected and transferred to a semi-hydroponic “channel” system. After four weeks in the seedbed, the total protein, peroxidase activity, nutrient content and rooting of the mini cuttings were evaluated. SDS-PAGE enabled the differentiation of leaf samples obtained by grafting from the other propagation techniques, as revealed by two distinct bands. Materials obtained by micropropagation with epicormic shoots showed the highest peroxidase activity, while those obtained by seminal propagation and from the selected mature tree showed the lowest peroxidase activity. A portable X-ray fluorescence spectroscope (pXRF) identified adequate nutrient content in most of the nutrients tested in materials obtained by seminal and grafting propagation, and by indirect organogenesis. The analysis of adventitious rooting showed that the highest rooting percentage was observed in mini cuttings from seminal propagation (75%) followed by indirect organogenesis (35%). Based on principal component analysis, it was concluded that rooting of mini cuttings from both seminal propagation and indirect organogenesis was associated with phosphorous, sulphur, and potassium contents, which suggests a higher level of tissue rejuvenation/reinvigoration in these propagated plants. Further studies are recommended to search for other methods that present similarities with the responses to adventitious rooting in forest species and thus optimize the rescue and propagation of plants with distinct ontogenetic stages.

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In vitro serial subculture to improve rooting of Eucalyptus urophylla

Cited by 10 publications

References 30 publications

Machine Learning Unmasked Nutritional Imbalances on the Medicinal Plant Bryophyllum sp. Cultured in vitro

Machine Learning Unmasked Nutritional Imbalances on the Medicinal Plant Bryophyllum sp. Cultured in vitro

Adventitious rooting and anatomical aspects of Campomanesia phaea stems

Evaluation of total protein, peroxidase, and nutrients measured by pXRF for the determination of tissue rejuvenation/reinvigoration of Eucalyptus microcorys

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